Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 75133
View all TrkB Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TrkB
Cys32-His430
Accession # Q16620

Specificity

Detects human TrkB in direct ELISAs. Detects human, mouse, and rat TrkB in Western blots. In ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TrkA, rhTrkC, or recombinant rat TrkA is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat TrkB Antibody

Detection of Human, Mouse, and Rat TrkB antibody by Western Blot.

Detection of Human, Mouse, and Rat TrkB by Western Blot.

Western blot shows lysates of human brain (motor cortex) tissue, mouse brain (cortex) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat TrkB Monoclonal Antibody (Catalog # MAB397) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TrkB at approximately 95 kDa and 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TrkB antibody in Human Brain by Immunohistochemistry (IHC-P).

TrkB in Human Brain.

TrkB was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human/Mouse/Rat TrkB Monoclonal Antibody (Catalog # MAB397) at 25 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human TrkB by Western Blot

Detection of Human TrkB by Western Blot

Expression of BDNF and TrkB in CRC cells in vitro.(A): RT-PCR analysis of BDNF and TrkB mRNA levels in CRC cell lines CaCO2 (lane: 1), DLD1 (lane: 2), HT29 (lane: 3), LoVo (lane: 4), and SW480 (lane: 5). GAPDH mRNA levels was used as an internal control. TrkB mRNA levels contain both TrkB.FL and TrkB.T1 mRNAs. (B): Western blotting analysis of BDNF and TrkB protein levels in CRC cell lines CaCO2 (lane: 1), DLD1 (lane: 2), HT29 (lane: 3), LoVo (lane: 4), and SW480 (lane: 5). The full-length TrkB (TrkB.FL, 145 kDa) and the truncated TrkB (TrkB.T1, 95 kDa) were detected. Actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24801982), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human TrkB by Immunocytochemistry/Immunofluorescence

Detection of Human TrkB by Immunocytochemistry/Immunofluorescence

BDNF and TrkB protein expression in primary and metastatic colorectal cancer.Representative images of immuno-reactive BDNF and TrkB protein expression in primary CRC and peritoneal metastasis are shown (original magnification: 100×). Anti TrkB antibody (R&D Systems, Foster City, CA, USA) detected both TrkB.FL and TrkB.T1 proteins, which was confirmed by Western blotting analysis. (A): The immunoreactive BDNF protein is located in the cytoplasm of the tumor cells of the primary CRC. (B): The immunoreactive BDNF protein is located in the cytoplasm of the tumor cells in the corresponding peritoneal metastasis. (C): The immunoreactive TrkB protein is located in the nucleus of the tumor cells of the primary CRC. (D): The immunoreactive TrkB protein is located in the nucleus of the tumor cells in the corresponding peritoneal metastasis. These expression patterns for the BDNF and TrkB proteins were confirmed in CRC patients (n = 5) whose primary and peritoneal metastatic nodules were available for immunohistochemistry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24801982), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human TrkB by Immunocytochemistry/Immunofluorescence

Detection of Human TrkB by Immunocytochemistry/Immunofluorescence

BDNF and TrkB protein expression in primary and metastatic colorectal cancer.Representative images of immuno-reactive BDNF and TrkB protein expression in primary CRC and peritoneal metastasis are shown (original magnification: 100×). Anti TrkB antibody (R&D Systems, Foster City, CA, USA) detected both TrkB.FL and TrkB.T1 proteins, which was confirmed by Western blotting analysis. (A): The immunoreactive BDNF protein is located in the cytoplasm of the tumor cells of the primary CRC. (B): The immunoreactive BDNF protein is located in the cytoplasm of the tumor cells in the corresponding peritoneal metastasis. (C): The immunoreactive TrkB protein is located in the nucleus of the tumor cells of the primary CRC. (D): The immunoreactive TrkB protein is located in the nucleus of the tumor cells in the corresponding peritoneal metastasis. These expression patterns for the BDNF and TrkB proteins were confirmed in CRC patients (n = 5) whose primary and peritoneal metastatic nodules were available for immunohistochemistry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24801982), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human TrkB by Western Blot

Detection of Human TrkB by Western Blot

Expression of BDNF and TrkB in CRC cells in vitro.(A): RT-PCR analysis of BDNF and TrkB mRNA levels in CRC cell lines CaCO2 (lane: 1), DLD1 (lane: 2), HT29 (lane: 3), LoVo (lane: 4), and SW480 (lane: 5). GAPDH mRNA levels was used as an internal control. TrkB mRNA levels contain both TrkB.FL and TrkB.T1 mRNAs. (B): Western blotting analysis of BDNF and TrkB protein levels in CRC cell lines CaCO2 (lane: 1), DLD1 (lane: 2), HT29 (lane: 3), LoVo (lane: 4), and SW480 (lane: 5). The full-length TrkB (TrkB.FL, 145 kDa) and the truncated TrkB (TrkB.T1, 95 kDa) were detected. Actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24801982), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human TrkB by Immunohistochemistry

Detection of Human TrkB by Immunohistochemistry

Expression of BDNF receptors in human placental tissues. (A) Representative confocal immunofluorescence staining images of showing DAPI (blue) and SDC1 (a syncytiotrophoblast biomarker, green) along with TrkB (red) and p75NTR (red) receptors in the first trimester placenta. (B) Confocal immunofluorescence staining images showing DAPI, SDC1, TrkB, and p75NTR in the term placenta. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34394001), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human TrkB by Immunohistochemistry

Detection of Human TrkB by Immunohistochemistry

Expression of BDNF receptors in human placental tissues. (A) Representative confocal immunofluorescence staining images of showing DAPI (blue) and SDC1 (a syncytiotrophoblast biomarker, green) along with TrkB (red) and p75NTR (red) receptors in the first trimester placenta. (B) Confocal immunofluorescence staining images showing DAPI, SDC1, TrkB, and p75NTR in the term placenta. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34394001), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TrkB by Western Blot

Detection of Mouse TrkB by Western Blot

7,8-DHF alleviates HFD-induced depression-like behaviors in Thy1-C/EBP beta Tg mice. (A) Schematic of the HFD and chow diet, 7,8-DHF and vehicle treatment, and behavioral tests process in wild-type and Thy1-C/EBP beta Tg male mice. (B) Representative immunoblots and (C) quantification of pTrkB and TrkB protein expression in the hippocampus of the above mice after 7,8-DHF or vehicle treatment. Data in (B) are representative of three independent experiments. Data in (C) represent the mean ± SEM (n = 6 for each group; **p < 0.01; one-way ANOVA and Bonferroni’s multiple comparison test). (D) Tail suspension test, (E) forced swim test and (F,G) sucrose preference test results for the above mice. Data represent the mean ± SEM (n = 10 mice for each group; *p < 0.05, **p < 0.01; NS, not significant; one-way ANOVA and Bonferroni’s multiple comparison test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36578534), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TrkB by Western Blot

Detection of Mouse TrkB by Western Blot

7,8-DHF alleviates HFD-induced depression-like behaviors in Thy1-C/EBP beta Tg mice. (A) Schematic of the HFD and chow diet, 7,8-DHF and vehicle treatment, and behavioral tests process in wild-type and Thy1-C/EBP beta Tg male mice. (B) Representative immunoblots and (C) quantification of pTrkB and TrkB protein expression in the hippocampus of the above mice after 7,8-DHF or vehicle treatment. Data in (B) are representative of three independent experiments. Data in (C) represent the mean ± SEM (n = 6 for each group; **p < 0.01; one-way ANOVA and Bonferroni’s multiple comparison test). (D) Tail suspension test, (E) forced swim test and (F,G) sucrose preference test results for the above mice. Data represent the mean ± SEM (n = 10 mice for each group; *p < 0.05, **p < 0.01; NS, not significant; one-way ANOVA and Bonferroni’s multiple comparison test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36578534), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat TrkB Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate and brain

Western Blot

2 µg/mL
Sample: Human brain (motor cortex) tissue, Mouse brain (cortex) tissue, and Rat brain (hippocampus) tissue

Reviewed Applications

Read 1 review rated 5 using MAB397 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

References

  1. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  2. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
  3. Soppet, D. et al. (1991) Cell 65:895.

Long Name

Neurotrophic Tyrosine Kinase Receptor B

Alternate Names

NTRK2

Entrez Gene IDs

4915 (Human); 18212 (Mouse); 25054 (Rat)

Gene Symbol

NTRK2

UniProt

Q16620

Additional TrkB Products

Product Documents for Human/Mouse/Rat TrkB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat TrkB Antibody

For research use only

Citations for Human/Mouse/Rat TrkB Antibody

Customer Reviews for Human/Mouse/Rat TrkB Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human/Mouse/Rat TrkB Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human/Mouse/Rat TrkB Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Hippocampal neurons
    Species: Mouse
    Verified Customer | Posted 10/16/2021
    Human/Mouse/Rat TrkB Antibody MAB397

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies