TrkB in Human Brain. TrkB was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human TrkB Monoclonal Antibody (Catalog # MAB397) at 25 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group
of structurally related, secreted proteins that play an important role
in the development and function of the nervous system. The biological
activities of the neurotrophins are mediated by binding to the different
members of the Trk family tyrosine kinase receptors. Three Trk family
proteins, TrkA, TrkB, and TrkC, exhibiting different ligand
specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and
NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved
complex subdomain organization consisting of a signal peptide, two
cysteine-rich domains, a cluster of three leucine-rich motifs, and two
immunoglobulin-like domains in the extracellular region, as well as an
intracellular region that contains the tyrosine kinase domain. Natural
splice variants of the different Trks, including TrkB variants lacking
the first cysteine-rich domain, the first and second or all three of the
leucine-rich motifs, or the tyrosine kinase domain, have been
described. The role of the different extracellular subdomains of TrkB in
mediating neurotrophin binding and discrimination is currently being
investigated. At the protein sequence level, human and rat TrkB are
greater than 90% identical and the proteins exhibit cross-species
activity. TrkB is primarily expressed in the nervous system. However,
low levels of TrkB expression have also been observed in a wide variety
of tissues (pancreas, kidneys, ovary) outside the nervous system.
Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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