Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Neutralization, IHC-Pr

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TSLPR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TSLP R
Val30-Phe232
Accession # Q9HC73

Specificity

Detects human TSLP R in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.100 EU per 1 µg of the antibody by the LAL method.

Scientific Data Images for Human TSLPR Antibody

Detection of Human TSLP R antibody by Western Blot.

Detection of Human TSLP R by Western Blot.

Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human TSLP R. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human TSLP R antibody by Western Blot.

Detection of Human TSLP R by Western Blot.

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TSLP R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

TSLP R antibody in Human Lymph Node by Immunohistochemistry (IHC-P).

TSLP R in Human Lymph Node.

TSLP R was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to the plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

TSLPR in THP‑1 Human Cell Line.

TSLPR was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line treated with PMA (positive staining) and K562 human chronic myelogenous leukemia cell line (negative staining) using Goat Anti-Human TSLPR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by TSLP and Neutralization by Human TSLP R Antibody.

Cell Proliferation Induced by TSLP and Neutralization by Human TSLP R Antibody.

Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-7 Ra and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.3 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981). The ND50 is typically 1-5 µg/mL.

Applications for Human TSLPR Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line treated with PMA

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node 

Western Blot

0.2-1 µg/mL
Sample: Human liver tissue, NS0 mouse myeloma cell line either mock transfected or transfected with human TSLP R

Neutralization

Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 0.3 ng/mL Recombinant Human TSLP.

Reviewed Applications

Read 2 reviews rated 4 using AF981 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSLPR

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS‑like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3-6). The TSLP R and IL-7 R alpha are coexpressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell‑attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5).

References

  1. Fujio, K. et al. (2000) Blood 95:2204.
  2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
  3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  4. Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
  5. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  6. Pandey, A. et al. (2000) Nat. Immunol. 1:59.

Long Name

Thymic Stromal Lymphopoietin Receptor

Alternate Names

CRL2, CRLF2, CRLM-2, PCOR1, TSLP R

Entrez Gene IDs

64109 (Human); 57914 (Mouse); 171499 (Rat)

Gene Symbol

CRLF2

UniProt

Q9HC73

Additional TSLPR Products

Product Documents for Human TSLPR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TSLPR Antibody

For research use only

Citations for Human TSLPR Antibody

Customer Reviews for Human TSLPR Antibody (2)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 20624642
    Species: Human
    Verified Customer | Posted 01/12/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 20624642
    Species: Human
    Verified Customer | Posted 01/12/2015

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