Mouse Syndecan-1/CD138 Antibody

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Syndecan‑1/CD138 in NMuMG Mouse Cell Line.
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Product Details
Citations (3)
Supplemental Products
Reviews (1)

Mouse Syndecan-1/CD138 Antibody Summary

Species Reactivity
Detects mouse Syndecan‑1/CD138 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Syndecan-1 is observed and less than 1% cross-reactivity with rhSyndecan-2, recombinant mouse (rm) Syndecan-3 and rmSyndecan-4 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Syndecan‑1/CD138 isoform 1
Accession # P18828
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Syndecan‑1/CD138 (Catalog # 3190-SD)
Flow Cytometry
2.5 µg/106 cells
T1165 mouse plasmacytoma cell line
3-15 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Syndecan-1/CD138 antibody in NMuMG Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Syndecan‑1/CD138 in NMuMG Mouse Cell Line. Syndecan-1/CD138 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using Mouse Syndecan-1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3190) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Syndecan-1/CD138 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Syndecan‑1/CD138 in Mouse Kidney. Syndecan-1/CD138 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Syndecan-1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3190) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-1/CD138

Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 311 amino acid (aa) precursor that contains a 17 aa signal sequence, a 235 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120‑200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. The amino acid sequence of mouse Syndecan-1 ECD shares 71% identity with that of the ECD of both rat and human Syndecan-1.

Entrez Gene IDs
6382 (Human); 20969 (Mouse); 100519770 (Porcine); 100338470 (Rabbit)
Alternate Names
CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1

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Citations for Mouse Syndecan-1/CD138 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Biodistribution and Cellular Internalization of Inactivated SARS-CoV-2 in Wild-Type Mice
    Authors: A Hudák, G Morgan, J Bacovsky, R Patai, TF Polgár, A Letoha, A Pettko-Sza, C Vizler, L Szilák, T Letoha
    International Journal of Molecular Sciences, 2022-07-09;23(14):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
    Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
    Sci Rep, 2020-07-31;10(1):12912.
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  3. Heparan sulfate proteoglycans present PCSK9 to the LDL receptor
    Authors: C Gustafsen, D Olsen, J Vilstrup, S Lund, A Reinhardt, N Wellner, T Larsen, CBF Andersen, K Weyer, JP Li, PH Seeberger, S Thirup, P Madsen, S Glerup
    Nat Commun, 2017-09-11;8(1):503.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


Reviews for Mouse Syndecan-1/CD138 Antibody

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Mouse Syndecan-1/CD138 Antibody
By Anonymous on 06/01/2016
Application: Flow Sample Tested: RPE-J Species: Rat