Porcine IL-6 Antibody

Catalog # Availability Size / Price Qty
MAB686
MAB686-SP
IL‑6 in Porcine PBMCs.
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Product Details
Citations (6)
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Porcine IL-6 Antibody Summary

Species Reactivity
Porcine
Specificity
Detects porcine IL-6 in direct ELISAs and Western blots. Approximately 2% cross-reactivity with recombinant mouse (rm) IL-6, recombinant rat IL-6, and rmLIF is observed.
Source
Monoclonal Mouse IgG1 Clone # 77830
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant porcine IL-6
Pro29-Met212
Accession # P26893
Formulation
Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Porcine IL‑6 (Catalog # 686-PI)
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry IL‑6 antibody in Porcine PBMCs by Immunocytochemistry (ICC). View Larger

IL‑6 in Porcine PBMCs. IL-6 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB686) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

References
  1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
  2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
  3. Jones, S.A. (2005) J. Immunol. 175:3468.
  4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
  5. Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.
  6. Murakami, M. et al. (1993) Science 260:1808.
  7. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  8. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Product Datasheets

Citations for Porcine IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Altered expression of cytokines IL-1?, IL-6, IL-8 and TNF-? in bovine follicular persistence
    Authors: AF Stassi, ME Baravalle, EM Belotti, F Rey, NC Gareis, PU Díaz, FM Rodríguez, CJ Leiva, HH Ortega, NR Salvetti
    Theriogenology, 2017;97(0):104-112.
    Species: Bovine
    Sample Types: Whole Tissue
    Applications: IHC
  2. Dendritic Cell Sensing of Hydrophobic Di- and Triacylated Lipopeptides Self-Assembled within Synthetic Virus-like Particles
    Authors: R Sharma, A Ghasparian, JA Robinson, KC McCullough
    J. Immunol., 2017;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant
    Authors: C Martin, SD Waghela, S Lokhandwal, A Ambrus, J Bray, C Vuong, V Vinodkumar, PJ Dominowski, S Rai, D Mwangi, DL Foss, W Mwangi
    PLoS ONE, 2017;12(1):e0170504.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Mutations in classical swine fever virus NS4B affect virulence in swine.
    Authors: Fernandez-Sainz I, Gladue DP, Holinka LG
    J. Virol., 2010;84(3):1536-49.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Low vasa vasorum densities correlate with inflammation and subintimal thickening: potential role in location--determination of atherogenesis.
    Authors: Gossl M, Versari D, Lerman LO, Chade AR, Beighley PE, Erbel R, Ritman EL
    Atherosclerosis, 2009;206(2):362-8.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC
  6. Sex difference in link between interleukin-6 and stress.
    Authors: Jankord R, Turk JR, Schadt JC, Casati J, Ganjam VK, Price EM, Keisler DH, Laughlin MH
    Endocrinology, 2007;148(8):3758-64.
    Species: Porcine
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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