Porcine IL-6 Antibody

(5 citations)   
  • Species Reactivity
    Porcine
  • Specificity
    Detects porcine IL-6 in direct ELISAs and Western blots. Approximately 2% cross-reactivity with recombinant mouse (rm) IL-6, recombinant rat IL-6, and rmLIF is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 77830
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant porcine IL-6
    Pro29-Met212
    Accession # P26893
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Porcine IL‑6 (Catalog # 686-PI)
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
IL‑6 in Porcine PBMCs. IL‑6 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Porcine IL‑6 Monoclonal Antibody (Catalog # MAB686) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

  • References:
    1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
    2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
    3. Jones, S.A. (2005) J. Immunol. 175:3468.
    4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
    5. Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.
    6. Murakami, M. et al. (1993) Science 260:1808.
    7. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
    8. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  • Long Name:
    Interleukin 6
  • Entrez Gene IDs:
    3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
  • Alternate Names:
    B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant
    Authors: C Martin, SD Waghela, S Lokhandwal, A Ambrus, J Bray, C Vuong, V Vinodkumar, PJ Dominowski, S Rai, D Mwangi, DL Foss, W Mwangi
    PLoS ONE, 2017;12(1):e0170504.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Flow
  2. Dendritic Cell Sensing of Hydrophobic Di- and Triacylated Lipopeptides Self-Assembled within Synthetic Virus-like Particles
    Authors: R Sharma, A Ghasparian, JA Robinson, KC McCullough
    J. Immunol., 2017;0(0):.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Functional Assay
  3. Mutations in classical swine fever virus NS4B affect virulence in swine.
    Authors: Fernandez-Sainz I, Gladue DP, Holinka LG
    J. Virol., 2010;84(3):1536-49.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Low vasa vasorum densities correlate with inflammation and subintimal thickening: potential role in location--determination of atherogenesis.
    Authors: Gossl M, Versari D, Lerman LO, Chade AR, Beighley PE, Erbel R, Ritman EL
    Atherosclerosis, 2009;206(2):362-8.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC
  5. Sex difference in link between interleukin-6 and stress.
    Authors: Jankord R, Turk JR, Schadt JC, Casati J, Ganjam VK, Price EM, Keisler DH, Laughlin MH
    Endocrinology, 2007;148(8):3758-64.
    Species: Porcine
    Sample Type: Tissue Homogenates
    Application: WB
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