Rat CD19 Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat CD19 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CD19 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 771404
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant rat CD19
    Met1-Gly287
    Accession # NP_001013255
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat CD19 by Western Blot. Western blot shows lysates of rat splenocytes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat CD19 Monoclonal Antibody (Catalog # MAB7489) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD19 at approximately 60-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
CD19 in Rat Spleen. CD19 was detected in perfusion fixed frozen sections of rat spleen using Mouse Anti-Rat CD19 Monoclonal Antibody (Catalog # MAB7489) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to B-cells inside the germinal layers. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD19

CD19 (also surface antigen B4 and Leu12) is a 95‑110 kDa member of the Immunoglobulin superfamily of molecules. It is expressed by B cells, and interacts with CD21 for the purpose of reducing the threshold of the antigen signal needed to with activate the BCR. CD19 ligation also promotes B cell:follicular dendritic cell (FDC) interaction and B cell proliferation in the FDC zone of the spleen. Mature rat CD19 is a 529 amino acid (aa) type I transmembrane glycoprotein (aa 19‑547). Based on mouse, it contains a 269 aa extracellular region (aa 19‑287) plus a 236 aa cytoplasmic domain. The extracellular region possesses two C2-type Ig-like domains (aa 20‑113 and 171‑271) and one utilized phosphorylation site at Ser225. The cytoplasmic domain contains five potential Tyr phosphorylation sites. There is one splice form that shows a two aa substitution after Gly489. Over aa 19‑287, rat CD19 shares 88% and 57% aa sequence identity with mouse and human CD19, respectively.

  • Entrez Gene IDs:
    930 (Human); 12478 (Mouse); 365367 (Rat); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)
  • Alternate Names:
    B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12
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