hh Rat VEGF Antibody AF564: R&D Systems

Rat VEGF Antibody

(19 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF165 and rhVEGF121 is observed and less than 2% cross-reactivity with rhVEGF-B, recombinant mouse (rm) VEGF-B, rhVEGF-C, rhVEGF-D, and rmVEGF-D is observed. In Western blots, detection of recombinant mouse VEGF165 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat VEGF164
    Ala27-Arg190
    Accession # AAL07526
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize VEGF164-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 20 ng/mL Recombinant Rat VEGF164.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Rat and Mouse VEGF by Western Blot. Western blot shows 25 ng of Recombinant Rat VEGF 164 (Catalog # 564-RV), Recombinant Human VEGF 165 (Catalog # 293-VE) and Recombinant Mouse VEGF 164 (Catalog # 493-MV). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for VEGF at approximately 20-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunohistochemistry
VEGF164 in Rat Kidney. VEGF164 was detected in perfusion fixed frozen sections of rat kidney using 15 µg/mL Goat Anti-Rat VEGF164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Cell Proliferation Induced by VEGF164 and Neutralization by Rat VEGF Antibody. Recombinant Rat VEGF164 (Catalog # 564-RV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat VEGF164 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564). The ND50 is typically 0.2-0.6 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by a cysteine-knot structure formed by eight conserved cysteine residues. Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164, and 188 aa isoforms found in rat and mouse. Isoforms other than VEGF120 and VEGF121 contain basic heparin-binding regions and are not freely diffusible. Rat VEGF164 shares 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 89% with porcine and canine, and 90% with feline and equine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the semaphorin receptor, neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M, and TNF-alpha.

  • Long Name:
    Vascular Endothelial Growth Factor
  • Entrez Gene IDs:
    7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
  • Alternate Names:
    MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Coronary angiogenic effect of long-term administration of Nigella sativa
    Authors: LI Al Asoom
    BMC Complement Altern Med, 2017;17(1):308.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  2. Genome-wide expression differences in anti-Vegf and dexamethasone treatment of inflammatory angiogenesis in the rat cornea
    Authors: P Mirabelli, A Mukwaya, A Lennikov, M Xeroudaki, B Peebo, M Schaupper, N Lagali
    Sci Rep, 2017;7(1):7616.
    Species: Rat
    Sample Type: In Vivo
    Application: Neut
  3. A multifunctional bioactive material that stimulates osteogenesis and promotes the vascularization bone marrow stem cells and their resistance to bacterial infection
    Authors: C Ma, Q Wei, B Cao, X Cheng, J Tian, H Pu, A Yusufu, L Cao
    PLoS ONE, 2017;12(3):e0172499.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  4. Renal primordia activate kidney regenerative events in a rat model of progressive renal disease.
    Authors: Imberti B, Corna D, Rizzo P, Xinaris C, Abbate M, Longaretti L, Cassis P, Benedetti V, Benigni A, Zoja C, Remuzzi G, Morigi M
    PLoS ONE, 2015;10(3):e0120235.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  5. FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy.
    Authors: Deliyanti D, Zhang Y, Khong F, Berka D, Stapleton D, Kelly D, Wilkinson-Berka J
    PLoS ONE, 2015;10(7):e0134392.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. Quantitative analyses of retinal vascular area and density after different methods to reduce VEGF in a rat model of retinopathy of prematurity.
    Authors: Wang H, Yang Z, Jiang Y, Flannery J, Hammond S, Kafri T, Vemuri S, Jones B, Hartnett M
    Invest Ophthalmol Vis Sci, 2014;55(2):737-44.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  7. The probiotic mixture VSL#3 accelerates gastric ulcer healing by stimulating vascular endothelial growth factor.
    Authors: Dharmani P, De Simone C, Chadee K
    PLoS ONE, 2013;8(3):e58671.
    Species: Rat
    Sample Type: Whole Tissue
    Application: Neut
  8. Long-term gene therapy with thrombospondin 2 inhibits TGF-beta activation, inflammation and angiogenesis in chronic allograft nephropathy.
    Authors: Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C
    PLoS ONE, 2013;8(12):e83846.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  9. Anti-VEGF antibody leads to later atypical intravitreous neovascularization and activation of angiogenic pathways in a rat model of retinopathy of prematurity.
    Authors: McCloskey M, Wang H, Jiang Y, Smith G, Strange J, Hartnett M
    Invest Ophthalmol Vis Sci, 2013;54(3):2020-6.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  10. Vascular endothelial growth factor is crucial for erythropoietin-induced improvement of cardiac function in heart failure.
    Authors: Westenbrink BD, Ruifrok WP, Voors AA
    Cardiovasc. Res., 2010;87(1):30-9.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  11. Production of scFv antibody fragments from a hybridoma with functional activity against human vascular endothelial growth factor.
    Authors: Irani Y, Brereton HM, Tilton RG
    Hybridoma (Larchmt), 2009;28(3):205-9.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Binding Assay
  12. VEGF promotes vascular sympathetic innervation.
    Authors: Marko SB, Damon DH
    Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2646-52.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  13. Heme oxygenase-1 promotes neovascularization in ischemic heart by coinduction of VEGF and SDF-1.
    Authors: Lin HH, Chen YH, Chang PF, Lee YT, Yet SF, Chau LY
    J. Mol. Cell. Cardiol., 2008;45(1):44-55.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  14. Neutralizing VEGF decreases tortuosity and alters endothelial cell division orientation in arterioles and veins in a rat model of ROP: relevance to plus disease.
    Authors: Hartnett ME, Martiniuk D, Byfield G, Geisen P, Zeng G, Bautch VL
    Invest. Ophthalmol. Vis. Sci., 2008;49(7):3107-14.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  15. Role of VEGF in maintaining renal structure and function under normotensive and hypertensive conditions.
    Authors: Advani A, Kelly DJ, Advani SL, Cox AJ, Thai K, Zhang Y, White KE, Gow RM, Marshall SM, Steer BM, Marsden PA, Rakoczy PE, Gilbert RE
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(36):14448-53.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  16. Enhancement of glucose transporter expression of brain endothelial cells by vascular endothelial growth factor derived from glioma exposed to hypoxia.
    Authors: Yeh WL, Lin CJ, Fu WM
    Mol. Pharmacol., 2007;73(1):170-7.
    Species: Rat
    Sample Type: Cell Culture Supernates
    Application: Neut
  17. Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy.
    Authors: Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H474-81.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  18. Effects of red wine polyphenols on postischemic neovascularization model in rats: low doses are proangiogenic, high doses anti-angiogenic.
    Authors: Baron-Menguy C, Bocquet A, Guihot AL, Chappard D, Amiot MJ, Andriantsitohaina R, Loufrani L, Henrion D
    FASEB J., 2007;21(13):3511-21.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  19. Inhibition of hypoxia-induced increase of blood-brain barrier permeability by YC-1 through the antagonism of HIF-1alpha accumulation and VEGF expression.
    Authors: Yeh WL, Lu DY, Lin CJ, Liou HC, Fu WM
    Mol. Pharmacol., 2007;72(2):440-9.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 19 Citations
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