Antibodies for SARS-CoV-2 Detection

Antibodies for SARS-CoV-2 Detection

R&D Systems offers a range of antibodies that recognize and bind various SARS-CoV-2 antigens, such as the S1 and S2 subunits, as well as the receptor binding domain (RBD). These antibodies are highly validated and can be used to detect SARS-CoV-2 in multiple sample types by a variety of applications including ELISA, flow cytometry, and immunohistochemistry. Explore our range of SARS-CoV-2 antibodies and learn more about a specific antibody by clicking its catalog number.

Additionally, visit our main Antibodies for Coronavirus Research page for more information on our antibodies and related resources for SARS-CoV-2 research.

Custom and Bulk Antibodies for COVID-19 Research

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SARS-CoV-2 Antibodies for Flow Cytometry

Detection of SARS-CoV-2 antigens by flow cytometry offers a range of uses for SARS-CoV-2 research and COVID-19 therapy development. Flow detection of the viral antigens in concert with live/dead cell stains or other conjugated antibodies, such as those for cellular markers, allows for the characterization of infected cells as well as screening potential therapeutics for antibody binding to infected cells. R&D Systems offers SARS-CoV-2 antibodies validated for flow cytometry for use in SARS-CoV-2 research and therapy development.

R&D Systems™ Anti-SARS-CoV-2 Antibodies for Flow Cytometry Staining
SARS-CoV-2 Antigen Clone Catalog # Conjugate
SARS-CoV-2 Spike S1 Subunit 1035206 MAB105403 Unconjugated
FAB105403G Alexa Fluor® 488
FAB105403N Alexa Fluor 700
FAB105403R Alexa Fluor 647
FAB105403S Alexa Fluor 750
FAB105403T Alexa Fluor 594
FAB105403U Alexa Fluor 350
FAB105403V Alexa Fluor 405

Detection of SARS-CoV-2 Spike Protein by Flow Cytometry. HEK293 human embryonic kidney cell line, transfected with human ACE-2 and eGFP, was incubated with Recombinant SARS-CoV-2 Spike S1 Subunit His-Tag Protein (Catalog # 10522-CV), then stained with a Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (A; Catalog # MAB105403) or a Mouse IgG1 Isotype Control Antibody (B; Catalog # MAB002), followed by an APC-Conjugated Goat Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). SARS-CoV-2 Spike protein, bound to ACE-2 expressed on the surface of the transfected HEK293 cells, was detected.

SARS-CoV-2 Spike Protein bound to ACE-2 on live, transfected HEK293 cells was detected by flow cytometry.

 

SARS-CoV-2 Antibodies for Immunohistochemistry (IHC)

Detection of SARS-CoV-2 by IHC is a vital tool for researching SARS-CoV-2. Visualization of the SARS-CoV-2 proteins within cells and tissues by IHC allows for the semiquantitative identification of the virus while maintaining the spatial context of its expression, such as cell types targeted by SARS-CoV-2. R&D Systems offers SARS-CoV-2 antibodies validated for IHC for characterizing SARS-CoV-2 infection.

R&D Systems Anti-SARS-CoV-2 Antibodies for IHC Staining
SARS-CoV-2 Antigen Clone Catalog #
SARS-CoV-2 Spike RBD 1035423 MAB105802
SARS-CoV-2 Spike S1 Subunit 1035206 MAB105403
SARS-CoV-2 Nucleocapsid 1035111 MAB10474
1035145 MAB104741

Spike RBD in SARS-CoV-2 Infected Human Lung. Spike RBD was detected in immersion-fixed, paraffin-embedded sections of SARS-CoV-2 infected human lung tissue using a Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB105802), followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Prior to incubation with the primary antibody, the tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). The tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.

Spike RBD in human lung tissue infected with SARS-CoV-2 detected with immunohistochemistry.

 

SARS-CoV-2 Antibodies for Western Blot

Immunoblotting is an invaluable tool for COVID-19 research. Investigating mechanisms that allow SARS-CoV-2 to infect cells and escape immune surveillance while maintaining high infectivity often utilizes in vitro cell models with western blot analysis of SARS-CoV-2 proteins to confirm SARS-CoV-2 infection. Western blot is also used to determine which protein antigens are recognized by potential therapies such as antibody-based vaccines or sera from convalescent patients. R&D Systems offers SARS-CoV-2 antibodies validated for western blot for use in SARS-CoV-2 research and therapy development.

R&D Systems Anti-SARS-CoV-2 Antibodies for Western Blot Detection
SARS-CoV-2 Antigen Clone Catalog #
SARS-CoV-2 Spike RBD 1034522 MAB10540
1034515 MAB105401
SARS-CoV-2 Spike S1 Subunit 1035206 MAB105403
SARS-CoV-2 Spike S2 Subunit 1034617 MAB10557
SARS-CoV-2 Nucleocapsid 1035111 MAB10474
1035145 MAB104741

Detection of SARS-CoV-2 Spike S2 Subunit and SARS-CoV-2 Spike (Active Trimer) by Western Blot. Western blot shows Recombinant SARS-CoV-2 Spike S2 Subunit Protein (Catalog # 10584-CV; left blot) and Recombinant SARS-CoV-2 Spike (Active Trimer) Protein (Catalog # 10549-CV; right blot). The PVDF membrane was probed with a Mouse Anti-SARS-CoV-2 Spike S2 Subunit Monoclonal Antibody (Catalog # MAB10557) followed by an HRP-Conjugated Donkey Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SARS-CoV-2 Spike S2 Subunit at approximately 70 kDa (as indicated).

Detection of SARS-CoV-2 Spike S2 Subunit and SARS-CoV-2 Spike trimer by Western Blot.