ELISA Kits

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R&D Systems ELISA Kits

R&D Systems^™ ELISA Kits are the most trusted and widely published ELISAs on the market with a menu of 1,000 target analytes spanning 16 species. Our ELISA Kits come in a variety of formats including complete, ready-to-use Quantikine^™ ELISAs, rapid 90-minute QuicKit^™ ELISAs, and the more versatile DuoSet^™ ELISA Development Systems that provide all the components necessary for a customer to develop their own working assay. Built using our own antibodies, proteins, and diluents, our ELISAs offer researchers the flexibility to choose from both off-the-shelf kits and a range of customized services, including lot reservations, bulk packaging, and the creation of new targets.

Our Quantikine Kits are complete, fully validated, ready-to-run immunoassay kits designed to measure protein concentrations in diverse sample types. Through meticulous development, Quantikine ELISA Kits deliver superior quantitative performance without requiring additional assay optimization by the customer. Every lot undergoes rigorous testing to ensure low background, a linear standard curve, consistent sensitivity, and a broad dynamic range. Control values established during assay development and validation ensure that our kits will perform consistently over time and are optimized to mitigate matrix effects and avoid false positives.

View All ELISA Kits

Which ELISA Kit is Right for Me?

Quantikine ELISA Kits

The Industry Gold
Standard

Optimized reagents and diluents for accurate sample values
Fully validated sample types: cell culture supernates, tissue lysates, serum, plasma, and more
Extensively tested for long-term consistency and reproducibility
Also available in QuantiGlo™ and IVD assay formats

Quantikine High Sensitivity

Detection of low-abundance biomarkers

Detect femtogram levels of protein
Complete ready-to-use kits
Exhaustively tested for superior quality and reproducibility
Detailed protocol booklets include inter- and intra-assay precision, recovery, linearity, and sensitivity

QuicKit™ ELISAs

Rapid, high throughput detection

Fast results in 90 minutes
Quantikine quality with lot-to-lot consistency and reproducibility
Simple two-step protocol
Growing menu of target analytes

DuoSet^® ELISA Kits

Cost-efficient ELISA development

ELISA reagents to design your own assay
More than 1000 targets and 16 model organisms
96 and 384-well ancillary reagent kit plates available
Adaptable to multiple platforms

In Vitro Diagnostic (IVD) Colorimetric ELISA Kits	Cytokine ELISA Kits	Protein A ELISA Kits
QuantiGlo® Chemiluminescent Sandwich ELISA Kits	Parameter Colorimetric Competitive ELISAs	DuoSet IC (IntraCellular) Assay Development Systems
DuoSet IC (Intracellular) Phospho-specific ELISA Development Systems	ELISA Kits for Unique Model Organisms	ELISA Natural and Recombinant Protein Controls

Quantikine vs. DuoSet ELISAs

Quantikine ELISA Kits are preconfigured and come in various formats to measure both intracellular and extracellular proteins using either alkaline phosphatase-based or polymeric streptavidin-HRP conjugate amplification systems. Extensively tested for long-term consistency and reproducibility, our master calibrated Quantikine assays guarantee consistent performance over time, and are optimized to minimize matrix effects and prevent false positives.
DuoSet ELISA Development Kits provide specific matched antibody pairs and the other essential components needed for immunoassay development. DuoSet ELISA Development Systems offer a cost-effective solution compared to purchasing individual antibodies and proteins and streamline the assay optimization process.

Ella™ Automated ELISA

The automated Ella™ platform brings a higher level of precision and workflow efficiency to your lab. Using a unique microfluidic cartridge design, Ella's Simple Plex™ assays offer rapid, hands-free, and reproducible multiplex testing for up to 8 analytes simultaneously. The automated cartridge design reduces user error, ensuring consistent and reproducible results across different users and sites. All cartridge formats feature simple setup, preloaded calibration curves, and results in less than 90 minutes.

Meet Ella

Compact ELISA Plate Reader

The Simple Reader™ has a small footprint, lightweight design, and time-efficient read-out. It offers a cost-effective alternative to larger, more expensive options, delivering precise and accurate results with minimal maintenance and user-friendly software. Unlike bulkier alternatives, the Simple Reader saves space, money, and time, making it ideal for streamlined ELISA workflows.

Empower every lab member with their own Simple Reader, featuring:

Compact Design: Take this personal plate reader on the go to any lab, anywhere.
4 LED Light Sources: 450 nm, 490 nm, 540 nm,
595 nm
96 Detection Units: Robust, reliable results every time.
Fast Read Time: Speed up data analysis by reading your plate in under 5 seconds.

Get Started with Simple Reader

ELISA Kit - Quality Testing Examples

Human IFN-gamma ELISA-Quantikine QuicKit

Human IFN-alpha 2 Quantikine High Sensitivity ELISA Kit

Human E-Cadherin Quantikine ELISA Kit

NucA Nuclease DuoSet ELISA

Human PD-1 QuicKit ELISA

Human/Mouse/Rat/Porcine/Canine TGF-beta ELISA - Quantikine

Custom and Bulk ELISA Services

Harness the unique experience of R&D Systems Quantikine ELISA team. Leverage our industry-leading expertise to accelerate your custom project. We support your project from feasibility to manufacturing. Our custom ELISA services include bulk packaging, lot reservations, ELISA Kit modifications and new target development. Our scientists have developed hundreds of unique diluents and have decades of experience optimizing conditions to detect a range of analytes and biomarkers.

R&D Systems Custom ELISA Services

Custom and Bulk ELISA Services

ELISA Resources

Research Areas

Immune Response Profiling	Animal Models	Immuno-Oncology
Cell and Gene Therapy	Immune Checkpoint	Type 1 Interferon

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ELISA Kit Supplier of the Year

R&D Systems has demonstrated outstanding support for the life science research community by providing ELISA Kits that are frequently cited in published research. The team's dedication has earned them two prestigious industry awards: CiteAb's "ELISA Kit Supplier of the Year" and Bioz's "Top Star™" Award, recognizing the brand's extensive citation database and leadership in the ELISA category. With reagent data covering nearly one million ELISA Kit products, R&D Systems continues to be a trusted partner in advancing scientific discovery.

CiteAB 2024 Award

What Is an ELISA?

ELISA, (enzyme-linked immunosorbent assays) are used to quantify specific target levels in various sample types. They typically employ 96-well plates coated with a capture antibody specific to the target protein or another biomolecule. After incubation, a detection antibody binds to a different epitope on the target antigen, forming a sandwich. A substrate is added and produces a signal proportional to the bound analyte. ELISA assays are often preferred over other assays due to their sensitivity, specificity, accuracy, and resilience to harsh conditions. Careful antibody selection, adherence to stringent manufacturing standards, and thorough testing and validation are essential steps to ensuring your ELISA delivers superior precision, accuracy, and sensitivity.
Learn More About ELISA Kits

R&D Systems ELISA Kits