TGF‑ beta 3 Antibody

Name: TGF‑ beta 3 Antibody
Brand: R&D Systems
SKU: MAB643
Price: 499 USD
Availability: InStock

R&D Systems | Catalog # MAB643

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Citations (11)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 44922

View all TGF-beta 3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TGF‑ beta 3
Ala301-Ser412 (Tyr340Phe)
Accession # NP_003230.1

Specificity

Detects TGF-beta 3 from multiple species in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) TGF-beta 1or rhTGF-beta 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for TGF‑ beta 3 Antibody

TGF-beta 3 in Human Heart.

TGF-beta 3 was detected in immersion fixed paraffin-embedded sections of human heart using TGF-beta 3 Monoclonal Antibody (Catalog # MAB643) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for TGF‑ beta 3 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Western Blot

1 µg/mL
Sample: Recombinant Human TGF-beta 3 (Catalog # 243-B3)
under non-reducing conditions only

Human TGF-beta 3 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: TGF‑ beta 3 Biotinylated Antibody (Catalog # BAF243)
Standard: Recombinant Human TGF-beta 3 Protein (Catalog # 243-B3)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TGF-beta 3

The active form of TGF-beta 3 is a disulfide-linked homodimer which is generated by activation of the latent TGF-beta 3 complex. TGF-beta s are multifunctional cytokines that activate or repress multiple genes in a wide variety of cell types. Depending on the cell context, TGF-beta can control many cellular processes including growth, morphogenesis, and apoptosis.