Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Neutralization

Cited:

Immunohistochemistry, Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TGF-beta 3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant chicken TGF‑ beta 3
Ala301-Ser412 (Tyr340Phe)
Accession # P10600

Specificity

Detects TGF-beta 3 in direct ELISAs. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for TGF‑ beta 3 Antibody

TGF‑ beta 3 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 3 Antibody.

TGF‑ beta 3 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 3 Antibody.

Recombinant Human TGF-beta 3 (243-B3) inhibits Recombinant Mouse IL-4 (404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 3 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of TGF-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA). The ND50 is typically 0.01-0.05 µg/mL.

TGF‑ beta 3 in Human Brain.

TGF‑ beta 3 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-TGF‑ beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human TGF-beta 3 by Western Blot

Detection of Human TGF-beta 3 by Western Blot

BCRL results in increased TGF‐ beta 1 expression and signaling. (A) Representative IF localisation of TGF‐ beta 1 (top) and pSMAD3 (bottom) in normal and lymphedematous (labelled LE) tissues. (B) Quantification of TGF‐ beta 1 (top) and pSMAD3 (bottom) IF staining areas in tissue sections of patients with unilateral BCRL. Each circle represents an average of three HPF views per patient (N = 8). (C) mRNA expression of TGF‐ beta isoforms and TGF‐ beta RI comparing normal and lymphedematous limb of patients with unilateral BCRL. Each circle represents an individual patient (N = 14). (D) Representative Western blot of TGF‐ beta isoforms, pSMAD3 and tSMAD3 in normal and lymphedematous limbs of patients with unilateral BCRL. (E) Quantification of Western blots with relative changes comparing normal and lymphedematous limb of each patient. Each circle represents an average of two separate Western blots per patient (N = 8). BCRL, breast cancer‐related lymphedema; TGF‐ beta 1, transforming growth factor‐beta 1; IF, immunofluorescence; LE, lymphedema; HPF, high‐power field; TGF‐ beta R‐I, transforming growth factor‐beta receptor I Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35652284), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for TGF‑ beta 3 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain

Neutralization

Measured by its ability to neutralize TGF‑ beta 3 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 0.01‑0.05 µg/mL in the presence of 0.1 ng/mL Recombinant Human TGF‑ beta 3 and 7.5 ng/mL Recombinant Mouse IL‑4.

Reviewed Applications

Read 1 review rated 5 using AF-243-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 3

TGF-beta 3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 3, mice with targeted deletion show defects palatogenesis and pulmonary development (2). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 220 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 3 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex (8‑10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 3 shows 100%, 99% and 98% aa identity with mouse/dog/horse, rat and pig TGF-beta 3, respectively. It demonstrates cross-species activity (1). TGF-beta 3 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1.This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta  RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

References

  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1988) EMBO J. 7:3737.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Long Name

Transforming Growth Factor beta 3

Alternate Names

ARVD1, LDS5, RNHF, TGFB3, TGFbeta 3

Entrez Gene IDs

7043 (Human); 21809 (Mouse); 25717 (Rat)

Gene Symbol

TGFB3

UniProt

P10600

Additional TGF-beta 3 Products

Product Documents for TGF‑ beta 3 Antibody

Certificate of Analysis

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Product Specific Notices for TGF‑ beta 3 Antibody

For research use only

Citations for TGF‑ beta 3 Antibody

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  • TGF-beta 3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human MCF-7 cell lysate
    Species: Human
    Verified Customer | Posted 04/09/2017
    Expression of TGF-beta 3 in breast cancer cell lysate using Goat anti-Human TGF-beta 3 antibody (#AF-243) at 1 ug/mL, followed by Donkey anti-Goat secondary antibody at 1:1,000 dilution. PVDF membrane blocked with 5% BSA-PBS.
    TGF‑ beta 3 Antibody AF-243-NA

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