Quantikine QuicKit ELISA Kits
Get Quantikine Quality in a Fraction of the Time
Quantikine™ QuicKit™ ELISAs are rapid, ready-to-use sandwich ELISA kits designed to quantify specific protein biomarkers in biological samples in approximately 90 minutes. Their streamlined, single-wash workflow provides a simplified and reliable solution for efficient data generation, delivering results significantly faster than traditional ELISAs, which can require up to 4.5 hours. QuicKit ELISAs maintain the high-quality standards of R&D Systems Quantikine ELISAs, providing sensitive, accurate, and reproducible protein quantification while reducing hands-on time and accelerating results.
QuicKit ELISA Features
- Higher throughput: Generate quantitative results in 90 minutes
- Simplified protocol: Reduce hands-on time with a single wash step
- Traditional Quantikine performance: Maintain data quality comparable to traditional Quantikine ELISAs
- Sensitive and accurate quantitation: Confidently measure protein concentrations in complex biological samples
- Lot-to-lot consistency: Ensure reproducible performance across assay lots
- Reliable supply: Access dependable long-term product availability
QuicKit ELISA Menu
QuicKit ELISA Validated Sample Types
Validated sample types for Quantikine QuicKit ELISAs vary by assay but commonly include serum, plasma, and cell culture supernatants. QuicKit ELISAs are extensively optimized to minimize matrix effects that are frequently observed with complex sample matrices.
QuicKit ELISAs utilize an anti-tag antibody coated microplate. Standards, samples, and controls are added to the microplate, followed by the addition of an antibody cocktail containing Tag-conjugated capture antibodies and HRP-conjugated detection antibodies. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.
QuicKit ELISA Kit Components
These colorimetric ready-to-use ELISAs come with all the components necessary to run your assay. Kit contents may vary slightly. Please refer to the product-specific inserts for a complete list of contents for each individual assay:
- Anti-Tag Coated Plate
- Tag-Conjugated Capture Antibody Concentrate
- HRP-Conjugated Detection Antibody Concentrate
- Assay Diluent
- Calibrator Diluent
- Standard
- 25X Wash Buffer
- Substrate
- Stop Solution
Application Examples for QuicKit ELISAs
QuicKit ELISAs are used for the rapid quantification of cytokines, chemokines, growth factors, immune checkpoint proteins, and other protein biomarkers. Common applications include biomarker validation, translational research, immunotherapy studies, vaccine response monitoring, inflammation research, and other protein analysis workflows.
Quantikine QuicKit ELISAs support rapid quantification of candidate protein biomarkers across multiple samples, making them well-suited for biomarker validation studies. With a streamlined 90-minute workflow, they help researchers efficiently analyze clinically relevant samples and generate results faster for translational and pharmacodynamic biomarker research.
Quantikine QuicKit ELISAs enable rapid quantification of cytokines, chemokines, and other immune mediators associated with immune activation and therapeutic responses. Their fast turnaround time makes them well suited for immunotherapy, cell therapy, CAR-T cell research, and immune monitoring workflows that require efficient analysis of multiple samples.
Rapid measurement of cytokines and other immune biomarkers can support studies of vaccine-induced immune responses, immune activation, and response kinetics. The streamlined QuicKit workflow enables researchers to generate quantitative results quickly while maintaining the performance expected from Quantikine ELISAs.
Quantikine QuicKit ELISAs support the quantitative analysis of inflammatory cytokines, chemokines, and other biomarkers involved in acute and chronic inflammatory responses. Their rapid assay format helps researchers generate quantitative data quickly, supporting a wide range of inflammation research applications.
Data You Can Trust
Although Quantikine QuicKit ELISAs produce results in a fraction of the time of traditional ELISAs, quality data is not sacrificed for speed. QuicKit ELISAs are developed using the same rigorous quality and validation standards that researchers have come to expect from Quantikine ELISAs.
Linearity of Dilution
Linearity of dilution is a hallmark of the R&D Systems Quantikine ELISAs and QuicKits are no exception. Linearity is important for assessing matrix effects, confirming that the QuicKit ELISA accurately measures sample values. Poor linearity of dilution or extreme deviation from 100% indicates that matrix effects can artifactually enhance or interfere with analyte detection.
Figure 1. The IL-18 Quantikine QuicKit ELISA has superior linearity to Supplier A. At dilute concentrations, Supplier A is susceptible to matrix effects.
Figure 2. The R&D Systems IP-10 Quantikine QuicKit has superior linearity to the Supplier A counterpart.
Figure 3. The R&D Systems G-CSF Quantikine QuicKit has superior linearity to the Supplier A counterpart.
Spike and Recovery
In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices. Matrix effects occur when a substance in the sample interferes with the accurate detection of a target analyte. Matrix effects are caused by biological phenomena such as non-specific antibody binding, antibody cross reactivity or interference. Spike and recovery experiments are an important tool for assessing the accuracy of your measurement.
Figure 4. R&D Systems IL-18 Quantikine QuicKit ELISAs have superior recovery to Supplier A in serum and plasma.
Figure 5. R&D Systems TNF-alpha Quantikine QuicKit ELISAs have superior recovery to Supplier A in serum and plasma.
All ELISA Kits and Services
R&D Systems has been developing ELISA kits for over 30 years. We have a wide range of assays available, from our Quantikine colorimetric ready-to-use kits to our DuoSet™ ELISA Development Systems and Novus ELISAs, spanning 12 different species and over 700 different targets. Still can’t find what you are looking for? We also use our development expertise to develop custom ELISA kits.
Not sure which ELISA kit you need for your research? Use our handy immunoassay selection tool to help you narrow down the ELISA kit that is right for you.
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A Quantikine QuicKit ELISA (enzyme-linked immunosorbent assay) is a rapid sandwich ELISA designed to deliver quantitative protein measurements in approximately 90 minutes. These complete, ready-to-use ELISA kits feature a simplified single-wash protocol that reduces assay time and hands-on effort while maintaining the sensitivity, accuracy, and reproducibility researchers expect from Quantikine ELISAs. This combination of speed and simplicity makes QuicKit ELISAs ideal for researchers who need reliable quantitative protein measurements on an accelerated timeline.
QuicKit ELISAs are designed to simplify and accelerate protein quantification by using a streamlined single-wash protocol that delivers quantitative results in around 90 minutes. Compared with traditional ELISAs, which often require multiple wash and incubation steps, QuicKit ELISAs reduce assay complexity and shorten time to results while maintaining the sensitivity, accuracy, and reproducibility expected from Quantikine ELISAs.
Quantikine QuicKit ELISAs use a simplified assay format that reduces the number of processing steps required to generate quantitative results. Fewer assay steps and plate washes shorten the overall assay duration, allowing quantitative protein measurements to be completed in approximately 90 minutes.
QuicKit ELISAs are particularly valuable when rapid protein quantification is needed without compromising data quality. They are well suited for biomarker screening, immunology research, time-sensitive experiments, and studies involving multiple sample collections.
Quantikine QuicKit ELISAs are available for a broad range of cytokines, chemokines, growth factors, immune checkpoint proteins, inflammatory mediators, and disease biomarkers including human IFN-γ, IFN-β, IL-1β, IL-2, IL-6, IL-10, IL-17, TNF-α, CXCL10/IP-10, CRP, G-CSF, VEGF, PD-1, PD-L1, and many others.
Validated sample types for Quantikine QuicKit ELISAs vary by analyte and kit but commonly include serum, plasma, and cell culture supernatants. Researchers should consult the product-specific datasheet for validated sample types, sample preparation recommendations, and assay performance data.
Yes, QuicKit ELISAs provide quantitative protein measurements using standard curve-based analysis. A calibrated protein standard is used to generate a standard curve, and the absorbance value of each unknown sample is compared to the standard curve to determine the corresponding analyte concentration within the assay's validated range. Like traditional Quantikine ELISAs, QuicKit ELISAs generate numerical concentration values rather than qualitative positive or negative results, enabling accurate protein quantification and comparison across samples and experimental conditions.