Bovine IL-4 Antibody

  
  • Species Reactivity
    Bovine
  • Specificity
    Detects bovine IL-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant porcine IL-4  is observed, less than 5% cross-reactivity with recombinant human IL-4, recombinant feline IL-4, and recombinant canine IL-4 is observed, and less than 1% cross-reactivity with recombinant mouse IL-4, recombinant rat IL-4, and recombinant rhesus macaque IL-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant bovine IL‑4
    His25-Cys135
    Accession # P30367
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Bovine IL‑4 (Catalog # 2469-BL)
  • Immunocytochemistry
    10-20 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
IL‑4 in Bovine PBMCs. IL‑4 was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Bovine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2469) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Bovine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Alternate splicing generates two additional isoforms with internal deletions (5). Mature bovine IL-4 shares 60%, 91%, 93%, 78%, 55%, 39%, and 41% aa sequence identity with equine, goat, ovine, porcine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8‑11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

  • References:
    1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
    2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
    3. Heussler, V.T. et al. (1992) Gene 114:273.
    4. Redfield, C. et al. (1991) Biochemistry 30:11029.
    5. Waldvogel, A.S. et al. (2004) Vet. Immunol. Immunopathol. 97:53.
    6. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
    7. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
    8. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
    9. Corthay, A. (2006) Scand. J. Immunol. 64:93.
    10. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
    11. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
    12. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
  • Long Name:
    Interleukin 4
  • Entrez Gene IDs:
    3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
  • Alternate Names:
    B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
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