Human AMPK beta 1 Antibody

Catalog #: MAB11642 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11642-100
MAB11642-SP

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Detection of Human AMPK beta 1 by Western Blot.

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All AMPK beta 1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AMPK beta 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human AMPK beta 1 protein in Direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1092531

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human AMPKbeta1
Met1-Ile270
Accession # Q9Y478

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line

Simple Western

10 µg/mL

HEK293T human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line

Immunohistochemistry

0.5-25 µg/mL

Immersion fixed paraffin-embedded sections of human kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human AMPK beta 1 by Western Blot. Western Blot shows lysates of K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human AMPK beta 1 Monoclonal Antibody (Catalog # MAB11642) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AMPK beta 1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry

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Detection of AMPK beta 1 in Human Kidney. AMPK beta 1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human AMPK beta 1 Monoclonal Antibody (Catalog # MAB11642) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western

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Detection of Human AMPK beta 1 by Simple Western^TM. Simple Western shows lysates of HEK293T human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for AMPK beta 1 at approximately 52 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human AMPK beta 1 Monoclonal Antibody (Catalog # MAB11642). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMPK beta 1

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The beta 1 subunit is expressed at higher levels than the beta 2 subunit in liver, while beta 2 is more abundant than beta 1 in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase complex as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase beta 1

Entrez Gene IDs

5564 (Human); 19079 (Mouse); 83803 (Rat)

Alternate Names

5'-AMP-activated protein kinase beta-1 subunit; AMP-activated protein kinase beta subunit; AMPK beta -1 chain; AMPK beta 1; AMPK beta 1,5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPK; AMPKb; HAMPKb; MGC17785; PRKAB1; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1