Human Apolipoprotein C-II/ApoC2 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB4497-100
MAB4497-SP
Detection of Human Apolipoprotein C‑II/ApoC2 by Western Blot.
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Human Apolipoprotein C-II/ApoC2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Apolipoprotein C‑II/ApoC2 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2554A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived human Apolipoprotein C‑II/ApoC2
Thr23-Glu101
Accession # P02655
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
ELISA

This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human Apolipoprotein C‑II/ApoC2 Monoclonal Antibody (Catalog # MAB44971).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
Immunohistochemistry
0.3-25 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Apolipoprotein C-II/ApoC2 antibody by Western Blot. View Larger

Detection of Human Apolipoprotein C‑II/ApoC2 by Western Blot. Western blot shows lysates of human liver tissue and human plasma. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Apolipoprotein C-II/ApoC2 Monoclonal Antibody (Catalog # MAB4497) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Apolipoprotein C-II/ApoC2 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Apolipoprotein C-II/ApoC2 antibody in THP-1 Human Cell Line by Immunocytochemistry (ICC). View Larger

Apolipoprotein C‑II/ApoC2 in THP‑1 Human Cell Line. Apolipoprotein C-II/ApoC2 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (positive stain) and MCF-7 human breast cancer cell line (negative stain) using Rabbit Anti-Human Apolipoprotein C-II/ApoC2 Monoclonal Antibody (Catalog # MAB4497) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Apolipoprotein C-II/ApoC2 antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

Apolipoprotein C‑II/ApoC2 in Human Liver. Apolipoprotein C-II/ApoC2 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Apolipoprotein C-II/ApoC2 Monoclonal Antibody (Catalog # MAB4497) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human Apolipoprotein C‑II/ApoC2 ELISA Standard Curve. Recombinant Human Apolipoprotein C‑II/ApoC2 protein was serially diluted 2-fold and captured by Rabbit Anti-Human Apolipoprotein C‑II/ApoC2 Monoclonal Antibody (Catalog # MAB4497) coated on a Clear Polystyrene Microplate (DY990). Rabbit Anti-Human Apolipoprotein C‑II/ApoC2 Monoclonal Antibody (MAB44971) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein C-II/ApoC2

Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents >90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% and 62% aa identical to mouse and rat ProApoC2, respectively.

Entrez Gene IDs
344 (Human); 11813 (Mouse); 292697 (Rat)
Alternate Names
APC2; APOC2; Apo-CII; apoC-II; Apolipoprotein C2; Apolipoprotein CII; Apolipoprotein C-II; MGC75082

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