Human beta -I Tubulin Antibody

Detection of Human and Mouse beta -I Tubulin by Western Blot.

Western blot shows lysates of human platelets and mouse platelets. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human beta -I Tubulin Monoclonal Antibody (Catalog # MAB8527) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta -I Tubulin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

beta -I Tubulin in BG01V Human Embryonic Stem Cells.

beta -I Tubulin was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human beta -I Tubulin Monoclonal Antibody (Catalog # MAB8527) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of beta -I Tubulin in human tonsil.

beta -I Tubulin was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human beta -I Tubulin Monoclonal Antibody (Catalog # MAB8527) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.