Human BMP-1/PCP Antibody

Name: Human BMP-1/PCP Antibody
Brand: R&D Systems

(4 citations)

Datasheet / CoA / SDS

Western Blot

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Western Blot

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Product Details

Citations (4)

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Species Reactivity

Human
Specificity

Detects human BMP‑1/PCP direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TLL-2 is observed, and less than 1% cross-reactivity with rhBMP-2, -3, -3b, -4, -5, -6, -7, -8, -9, -10, and -15 is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMP‑1/PCP
Ala121-Gln730
Accession # NP_001190
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

0.1 µg/mL

See below
Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human BMP‑1/PCP (Catalog # 1927-ZN), see our available Western blot detection antibodies
Immunocytochemistry

5-15 µg/mL

Immersion fixed U2OS human osteosarcoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

click image to view larger

Detection of Recombinant Human BMP‑1/PCP by Western Blot. Western blot shows 25 ng of Recombinant Human BMP‑1/
PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human/Mouse/
Rat BMP‑2 (Catalog # 355-BM), Recombinant Human BMP‑3 (Catalog #
113-BP), Recombinant Human BMP‑3b/GDF‑10 (Catalog # 1543-BP), Recombinant Human BMP‑4 (Catalog #
314-BP), Recombinant Human BMP‑5 (Catalog # 615-BMC), Recombinant Human BMP‑6 (Catalog # 507-BP), Recombinant Human BMP‑7 (Catalog # 354-BP), Recombinant Human BMP‑8a (Catalog # 1073-BP), Recombinant Human BMP‑8b (Catalog # 9316-BP), Recombinant Human BMP‑9 (Catalog # 3209-BP), Recombinant Human BMP‑10 (Catalog # 2926-BP) and Recombinant Human BMP‑15/GDF‑9B (Catalog # 5096-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human BMP‑1/PCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BMP‑1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Western Blot

click image to view larger

Detection of Recombinant Human BMP‑1/PCP by Western Blot. Western blot shows 25 ng of Recombinant Human BMP‑1/
PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human Meprin alpha Subunit/MEP1A (Catalog #
3220-ZN), Recombinant Human Meprin beta Subunit/MEP1B (Catalog # 2895-ZN), Recombinant Human Ovastacin, Recombinant Human ADAM10 (Catalog # 936-AD), Recombinant Human ADAMTS4 (Catalog # 4307-AD), and Recombinant Human MMP‑1 (Catalog # 901-MP). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human BMP‑1/PCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BMP‑1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-1/PCP

Bone morphogenetic protein 1 (BMP-1), also known as procollagen C-proteinase (PCP), is a zinc protease of the astacin family (1, 2). BMP-1/PCP plays a key role in formation of extracellular matrix (ECM) by converting precursor proteins into their mature and functional forms. The precursor proteins identified as substrates for BMP-1/PCP include collagens, biglycan, laminin 5, dentin matrix protein-1, and lysyl oxidase (3). There are six alternatively spliced forms known to be derived from the BMP-1 gene, and isoform 1 consisting of residues 1 to 730 was expressed. The secreted and purified protein does not contain the signal peptide (amino acid residues 1‑22) and pro domain (residues 23‑120), but contain protease (residues 121‑321), CUB I (residues 322‑434), CUB II (residues 435‑546), EGF-like (residues 547‑588) and CUB III (residues 591‑703) domains. The pro domain is apparently cleaved by a furin-like proprotein convertase (4). The purified BMP-1/PCP is an active protease and its peptidase activity can be determined as described above. The purified BMP-1/PCP is predicted to possess procollagen C-proteinase activity because it contains the minimal domain structure required (5).

References:
1. Wozney, J.M. et al. (1988) Science 242:1528.
2. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
3. Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
4. Leighton, M. and K.E. Kadler (2003) J. Biol. Chem. 278:18478.
5. Hartigan, N. et al. (2003) J. Biol. Chem. 278:18045.

Long Name:

Bone Morphogenetic Protein 1

Entrez Gene IDs:

649 (Human)

Alternate Names:

BMP1; BMP-1; bone morphogenetic protein 1; EC 3.4.24; EC 3.4.24.19; FLJ44432; Mammalian tolloid protein; mTld; PCOLC; PCP; PCP2; procollagen C-endopeptidase; Procollagen C-proteinase; TLD

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species	Applications	Sample Type
Defective Proteolytic Processing of Fibrillar Procollagens and Prodecorin Due to Biallelic BMP1 Mutations Results in a Severe, Progressive Form of Osteogenesis Imperfecta. Authors: Syx D, Guillemyn B, Symoens S, Sousa A, Medeira A, Whiteford M, Hermanns-Le T, Coucke P, De Paepe A, Malfait F J Bone Miner Res, 2015;30(8):1445-56. Species: Human Sample Type: Cell Culture Supernates Application: WB Isolation of a hemidesmosome-rich fraction from a human squamous cell carcinoma cell line. Authors: Hirako Y, Yonemoto Y, Yamauchi T, Nishizawa Y, Kawamoto Y, Owaribe K Exp Cell Res, 2014;324(2):172-82. Species: Human Sample Type: Cell Culture Supernates Application: WB Astacin proteases cleave dentin sialophosphoprotein (Dspp) to generate dentin phosphoprotein (Dpp). Authors: Tsuchiya S, Simmer JP, Hu JC, Richardson AS, Yamakoshi F, Yamakoshi Y J. Bone Miner. Res., 2010;26(0):220. Species: Porcine Sample Type: Tissue Homogenates Application: WB Dentin matrix protein-1 isoforms promote differential cell attachment and migration. Authors: Von Marschall Z, Fisher LW J. Biol. Chem., 2008;283(47):32730-40. Species: Human Sample Type: Recombinant Protein Application: WB

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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 17

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Human BMP-1/PCP Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

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