Human BMP-1/PCP Antibody
Human BMP-1/PCP Antibody Summary
Ala121-Gln730
Accession # NP_001190
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Detection of Recombinant Human BMP‑1/PCP by Western Blot. Western blot shows 25 ng of Recombinant Human BMP-1/ PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human/Mouse/ Rat BMP-2 (Catalog # 355-BM), Recombinant Human BMP-3 (Catalog # 113-BP), Recombinant Human BMP-3b/GDF-10 (Catalog # 1543-BP), Recombinant Human BMP-4 (Catalog # 314-BP), Recombinant Human BMP-5 (Catalog # 615-BMC), Recombinant Human BMP-6 (Catalog # 507-BP), Recombinant Human BMP-7 (Catalog # 354-BP), Recombinant Human BMP-8a (Catalog # 1073-BP), Recombinant Human BMP-8b (Catalog # 9316-BP), Recombinant Human BMP-9 (Catalog # 3209-BP), Recombinant Human BMP-10 (Catalog # 2926-BP) and Recombinant Human BMP-15/GDF-9B (Catalog # 5096-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human BMP-1/PCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BMP-1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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Detection of Recombinant Human BMP‑1/PCP by Western Blot. Western blot shows 25 ng of Recombinant Human BMP-1/ PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human Meprin a Subunit/MEP1A (Catalog # 3220-ZN), Recombinant Human Meprin beta Subunit/MEP1B (Catalog # 2895-ZN), Recombinant Human Ovastacin, Recombinant Human ADAM10 (Catalog # 936-AD), Recombinant Human ADAMTS4 (Catalog # 4307-AD), and Recombinant Human MMP-1 (Catalog # 901-MP). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human BMP-1/PCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BMP-1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-1/PCP
Bone morphogenetic protein 1 (BMP-1), also known as procollagen C-proteinase (PCP), is a zinc protease of the astacin family (1, 2). BMP-1/PCP plays a key role in formation of extracellular matrix (ECM) by converting precursor proteins into their mature and functional forms. The precursor proteins identified as substrates for BMP-1/PCP include collagens, biglycan, laminin 5, dentin matrix protein-1, and lysyl oxidase (3). There are six alternatively spliced forms known to be derived from the BMP-1 gene, and isoform 1 consisting of residues 1 to 730 was expressed. The secreted and purified protein does not contain the signal peptide (amino acid residues 1‑22) and pro domain (residues 23‑120), but contain protease (residues 121‑321), CUB I (residues 322‑434), CUB II (residues 435‑546), EGF-like (residues 547‑588) and CUB III (residues 591‑703) domains. The pro domain is apparently cleaved by a furin-like proprotein convertase (4). The purified BMP-1/PCP is an active protease and its peptidase activity can be determined as described above. The purified BMP-1/PCP is predicted to possess procollagen C-proteinase activity because it contains the minimal domain structure required (5).
- Wozney, J.M. et al. (1988) Science 242:1528.
- Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
- Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
- Leighton, M. and K.E. Kadler (2003) J. Biol. Chem. 278:18478.
- Hartigan, N. et al. (2003) J. Biol. Chem. 278:18045.
Product Datasheets
Citations for Human BMP-1/PCP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Enhancement of collagen deposition and cross-linking by coupling lysyl oxidase with bone morphogenetic protein-1 and its application in tissue engineering
Authors: T Rosell-Gar, F Rodriguez-
Sci Rep, 2018;8(1):10780.
Applications: Western Blot -
Defective Proteolytic Processing of Fibrillar Procollagens and Prodecorin Due to Biallelic BMP1 Mutations Results in a Severe, Progressive Form of Osteogenesis Imperfecta.
Authors: Syx D, Guillemyn B, Symoens S, Sousa A, Medeira A, Whiteford M, Hermanns-Le T, Coucke P, De Paepe A, Malfait F
J Bone Miner Res, 2015;30(8):1445-56.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Isolation of a hemidesmosome-rich fraction from a human squamous cell carcinoma cell line.
Authors: Hirako Y, Yonemoto Y, Yamauchi T, Nishizawa Y, Kawamoto Y, Owaribe K
Exp Cell Res, 2014;324(2):172-82.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Astacin proteases cleave dentin sialophosphoprotein (Dspp) to generate dentin phosphoprotein (Dpp).
Authors: Tsuchiya S, Simmer JP, Hu JC, Richardson AS, Yamakoshi F, Yamakoshi Y
J. Bone Miner. Res., 2010;26(0):220.
Species: Porcine
Sample Types: Tissue Homogenates
Applications: Western Blot -
Dentin matrix protein-1 isoforms promote differential cell attachment and migration.
Authors: Von Marschall Z, Fisher LW
J. Biol. Chem., 2008;283(47):32730-40.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot
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