Human BMP-1/PCP Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human BMP‑1/PCP direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TLL-2 is observed, and less than 1% cross-reactivity with rhBMP-2, -3, -3b, -4, -5, -6, -7, -8, -9, -10, and -15 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BMP‑1/PCP
    Ala121-Gln730
    Accession # NP_001190
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human BMP‑1/PCP (Catalog # 1927-ZN), see our available Western blot detection antibodies
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed U2OS human osteosarcoma cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human BMP‑1/PCP by Western Blot. Western blot shows 25 ng of Recombinant Human BMP‑1/
PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human/Mouse/
Rat BMP‑2 (Catalog # 355-BM), Recombinant Human BMP‑3 (Catalog #
113-BP), Recombinant Human BMP‑3b/GDF‑10 (Catalog # 1543-BP), Recombinant Human BMP‑4 (Catalog #
314-BP), Recombinant Human BMP‑5 (Catalog # 615-BMC), Recombinant Human BMP‑6 (Catalog # 507-BP), Recombinant Human BMP‑7 (Catalog # 354-BP), Recombinant Human BMP‑8a (Catalog # 1073-BP), Recombinant Human BMP‑8b (Catalog # 9316-BP), Recombinant Human BMP‑9 (Catalog # 3209-BP), Recombinant Human BMP‑10 (Catalog # 2926-BP) and Recombinant Human BMP‑15/GDF‑9B (Catalog # 5096-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human BMP‑1/PCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BMP‑1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Recombinant Human BMP‑1/PCP by Western Blot. Western blot shows 25 ng of Recombinant Human BMP‑1/
PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human Meprin  alpha  Subunit/MEP1A (Catalog #
3220-ZN), Recombinant Human Meprin  beta Subunit/MEP1B (Catalog # 2895-ZN), Recombinant Human Ovastacin, Recombinant Human ADAM10 (Catalog # 936-AD), Recombinant Human ADAMTS4 (Catalog # 4307-AD), and Recombinant Human MMP‑1 (Catalog # 901-MP). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human BMP‑1/PCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BMP‑1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-1/PCP

Bone morphogenetic protein 1 (BMP-1), also known as procollagen C-proteinase (PCP), is a zinc protease of the astacin family (1, 2). BMP-1/PCP plays a key role in formation of extracellular matrix (ECM) by converting precursor proteins into their mature and functional forms. The precursor proteins identified as substrates for BMP-1/PCP include collagens, biglycan, laminin 5, dentin matrix protein-1, and lysyl oxidase (3). There are six alternatively spliced forms known to be derived from the BMP-1 gene, and isoform 1 consisting of residues 1 to 730 was expressed. The secreted and purified protein does not contain the signal peptide (amino acid residues 1‑22) and pro domain (residues 23‑120), but contain protease (residues 121‑321), CUB I (residues 322‑434), CUB II (residues 435‑546), EGF-like (residues 547‑588) and CUB III (residues 591‑703) domains. The pro domain is apparently cleaved by a furin-like proprotein convertase (4). The purified BMP-1/PCP is an active protease and its peptidase activity can be determined as described above. The purified BMP-1/PCP is predicted to possess procollagen C-proteinase activity because it contains the minimal domain structure required (5).

  • References:
    1. Wozney, J.M. et al. (1988) Science 242:1528.
    2. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
    3. Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
    4. Leighton, M. and K.E. Kadler (2003) J. Biol. Chem. 278:18478.
    5. Hartigan, N. et al. (2003) J. Biol. Chem. 278:18045.
  • Long Name:
    Bone Morphogenetic Protein 1
  • Entrez Gene IDs:
    649 (Human)
  • Alternate Names:
    BMP1; BMP-1; bone morphogenetic protein 1; EC 3.4.24; EC 3.4.24.19; FLJ44432; Mammalian tolloid protein; mTld; PCOLC; PCP; PCP2; procollagen C-endopeptidase; Procollagen C-proteinase; TLD
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Defective Proteolytic Processing of Fibrillar Procollagens and Prodecorin Due to Biallelic BMP1 Mutations Results in a Severe, Progressive Form of Osteogenesis Imperfecta.
    Authors: Syx D, Guillemyn B, Symoens S, Sousa A, Medeira A, Whiteford M, Hermanns-Le T, Coucke P, De Paepe A, Malfait F
    J Bone Miner Res, 2015;30(8):1445-56.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  2. Isolation of a hemidesmosome-rich fraction from a human squamous cell carcinoma cell line.
    Authors: Hirako Y, Yonemoto Y, Yamauchi T, Nishizawa Y, Kawamoto Y, Owaribe K
    Exp Cell Res, 2014;324(2):172-82.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  3. Astacin proteases cleave dentin sialophosphoprotein (Dspp) to generate dentin phosphoprotein (Dpp).
    Authors: Tsuchiya S, Simmer JP, Hu JC, Richardson AS, Yamakoshi F, Yamakoshi Y
    J. Bone Miner. Res., 2010;26(0):220.
    Species: Porcine
    Sample Type: Tissue Homogenates
    Application: WB
  4. Dentin matrix protein-1 isoforms promote differential cell attachment and migration.
    Authors: Von Marschall Z, Fisher LW
    J. Biol. Chem., 2008;283(47):32730-40.
    Species: Human
    Sample Type: recombinant protein
    Application: WB
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