Human BMP-1/PCP Antibody
Human BMP-1/PCP Antibody Summary
Ala121-Gln730
Accession # NP_001190
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
View Larger
Detection of Recombinant Human BMP‑1/PCP by Western Blot.
Western blot shows 25 ng of Recombinant Human BMP‑1/
PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human/Mouse/
Rat BMP‑2 (Catalog # 355-BM), Recombinant Human BMP‑3 (Catalog #
113-BP), Recombinant Human BMP‑3b/GDF‑10 (Catalog # 1543-BP), Recombinant Human BMP‑4 (Catalog #
314-BP), Recombinant Human BMP‑5 (Catalog # 615-BMC), Recombinant Human BMP‑6 (Catalog # 507-BP), Recombinant Human BMP‑7 (Catalog # 354-BP), Recombinant Human BMP‑8a (Catalog # 1073-BP), Recombinant Human BMP‑8b (Catalog # 9316-BP), Recombinant Human BMP‑9 (Catalog # 3209-BP), Recombinant Human BMP‑10 (Catalog # 2926-BP) and Recombinant Human BMP‑15/GDF‑9B (Catalog # 5096-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human BMP‑1/PCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BMP‑1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
View Larger
Detection of Recombinant Human BMP‑1/PCP by Western Blot.
Western blot shows 25 ng of Recombinant Human BMP‑1/
PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human Meprin alpha Subunit/MEP1A (Catalog #
3220-ZN), Recombinant Human Meprin beta Subunit/MEP1B (Catalog # 2895-ZN), Recombinant Human Ovastacin, Recombinant Human ADAM10 (Catalog # 936-AD), Recombinant Human ADAMTS4 (Catalog # 4307-AD), and Recombinant Human MMP‑1 (Catalog # 901-MP). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human BMP‑1/PCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BMP‑1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-1/PCP
Bone morphogenetic protein 1 (BMP-1), also known as procollagen C-proteinase (PCP), is a zinc protease of the astacin family (1, 2). BMP-1/PCP plays a key role in formation of extracellular matrix (ECM) by converting precursor proteins into their mature and functional forms. The precursor proteins identified as substrates for BMP-1/PCP include collagens, biglycan, laminin 5, dentin matrix protein-1, and lysyl oxidase (3). There are six alternatively spliced forms known to be derived from the BMP-1 gene, and isoform 1 consisting of residues 1 to 730 was expressed. The secreted and purified protein does not contain the signal peptide (amino acid residues 1‑22) and pro domain (residues 23‑120), but contain protease (residues 121‑321), CUB I (residues 322‑434), CUB II (residues 435‑546), EGF-like (residues 547‑588) and CUB III (residues 591‑703) domains. The pro domain is apparently cleaved by a furin-like proprotein convertase (4). The purified BMP-1/PCP is an active protease and its peptidase activity can be determined as described above. The purified BMP-1/PCP is predicted to possess procollagen C-proteinase activity because it contains the minimal domain structure required (5).
- Wozney, J.M. et al. (1988) Science 242:1528.
- Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
- Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
- Leighton, M. and K.E. Kadler (2003) J. Biol. Chem. 278:18478.
- Hartigan, N. et al. (2003) J. Biol. Chem. 278:18045.
Product Datasheets
Citations for Human BMP-1/PCP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
5
Citations: Showing 1 - 5
Filter your results:
Filter by:
-
Enhancement of collagen deposition and cross-linking by coupling lysyl oxidase with bone morphogenetic protein-1 and its application in tissue engineering
Authors: T Rosell-Gar, F Rodriguez-
Sci Rep, 2018;8(1):10780.
Applications: Western Blot -
Defective Proteolytic Processing of Fibrillar Procollagens and Prodecorin Due to Biallelic BMP1 Mutations Results in a Severe, Progressive Form of Osteogenesis Imperfecta.
Authors: Syx D, Guillemyn B, Symoens S, Sousa A, Medeira A, Whiteford M, Hermanns-Le T, Coucke P, De Paepe A, Malfait F
J Bone Miner Res, 2015;30(8):1445-56.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Isolation of a hemidesmosome-rich fraction from a human squamous cell carcinoma cell line.
Authors: Hirako Y, Yonemoto Y, Yamauchi T, Nishizawa Y, Kawamoto Y, Owaribe K
Exp Cell Res, 2014;324(2):172-82.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Astacin proteases cleave dentin sialophosphoprotein (Dspp) to generate dentin phosphoprotein (Dpp).
Authors: Tsuchiya S, Simmer JP, Hu JC, Richardson AS, Yamakoshi F, Yamakoshi Y
J. Bone Miner. Res., 2010;26(0):220.
Species: Porcine
Sample Types: Tissue Homogenates
Applications: Western Blot -
Dentin matrix protein-1 isoforms promote differential cell attachment and migration.
Authors: Von Marschall Z, Fisher LW
J. Biol. Chem., 2008;283(47):32730-40.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsCell and Tissue Staining Kits
ExactaChIP Chromatin Immunoprecipitation Kits
Isotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human BMP-1/PCP Antibody
There are currently no reviews for this product. Be the first to review Human BMP-1/PCP Antibody and earn rewards!
Have you used Human BMP-1/PCP Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
