Human BMP-4 Antibody

(9 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human BMP-4 in direct ELISAs and Western blots. This antibody shows approximately 5% cross-reactivity with recombinant human BMP‑2.
  • Source
    Monoclonal Mouse IgG2B Clone # 66119
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BMP‑4
    Ser293-Arg408
    Accession # P12644
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human BMP‑4 (Catalog # 314-BP)
  • Neutralization
    Measured by its ability to neutralize BMP‑4-induced alkaline phosphatase production in the C2C12 mouse myoblast cell line. Katagiri, T. et al. (1994) J. Cell Biol. 127:1755. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 500 ng/mL Recombinant Human BMP‑4 and 2 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Alkaline Phosphatase Production Induced by BMP‑4 and Neutralization by Human BMP‑4 Antibody. Recombinant Human BMP‑4 (Catalog # 314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‑4 Monoclonal Antibody (Catalog # MAB757). The ND50 is typically 1-3 µg/mL in the presence of heparin (2 µg/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-4
BMP4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 5). The human BMP4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfidelinked heterodimer (7 9). Mature human and mouse BMP4 share 98% aa sequence identity. Human BMP4 shares 85% aa sequence identity with human BMP2 and less than 50% with other human BMPs. Compared to BMP4 homodimers, BMP4/ BMP7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13 - 15).
  • References:
    1. Zhang, P. et al., 2008, Blood 111:1933.
    2. Gambaro, K. et al., 2006, Cell Death Differ. 13:1075.
    3. Simic, P.  and Vukicevic, S., 2005, Cytokine Growth Factor Rev. 16:299.
    4. Sadlon, T.J. et al., 2004, Stem Cells 22:457.
    5. Frank, D.B. et al., 2005, Circ. Res. 97:496.
    6. Wozney, J. et al., 1988, Science 242:1528.
    7. Cui, Y. et al., 1998, EMBO J. 17:4735.
    8. Cui, Y. et al., 2001, Genes Dev. 15:2797.
    9. Aono, A. et al., 1995, Biochem. Biophys. Res. Commun. 210:670.
    10. Nishimatsu, S. and G.H. Thomsen, 1998, Mech. Dev. 74:75.
    11. Chen, D. et al., 2004, Growth Factors 22:233.
    12. Lavery, K. et al., 2008, J. Biol. Chem. 283:20948.
    13. Rosen, V., 2006, Ann. N.Y. Acad. Sci. 1068:19.
    14. Jones, C.M. and J.C. Smith, 1998, Dev. Biol. 194:12.
    15. Takada, T. et al., 2003, J. Biol. Chem. 278:43229.
  • Long Name:
    Bone Morphogenetic Protein 4
  • Entrez Gene IDs:
    652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
  • Alternate Names:
    BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; OFC11; ZYME
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells
    Authors: C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Type: Recombinant Proein
    Application: IP
  2. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
    Authors: Herrera B, Inman GJ,
    BMC Cell Biol., 2009;10(0):20.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Neut
  3. Chordin-like 1, a bone morphogenetic protein-4 antagonist, is upregulated by hypoxia in human retinal pericytes and plays a role in regulating angiogenesis.
    Authors: Kane R, Godson C, O'Brien C
    Mol. Vis., 2008;14(0):1138-48.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
    Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
    Blood, 2008;112(8):3154-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells.
    Authors: Zode GS, Clark AF, Wordinger RJ
    Invest. Ophthalmol. Vis. Sci., 2007;48(11):5058-67.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  6. Bone morphogenetic proteins promote gliosis in demyelinating spinal cord lesions.
    Authors: Fuller ML, DeChant AK, Rothstein B, Caprariello A, Wang R, Hall AK, Miller RH
    Ann. Neurol., 2007;62(3):288-300.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach.
    Authors: Bleuming SA, Kodach LL, Garcia Leon MJ, Richel DJ, Peppelenbosch MP, Reitsma PH, Hardwick JC, van den Brink GR
    J. Pathol., 2006;209(2):190-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation.
    Authors: Moser M, Binder O, Wu Y, Aitsebaomo J, Ren R, Bode C, Bautch VL, Conlon FL, Patterson C
    Mol. Cell. Biol., 2003;23(16):5664-79.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Neut
  9. Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
    Authors: van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ, Peppelenbosch MP
    Gastroenterology, 2001;121(2):317-28.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Isotype Controls
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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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