Key Product Details

Species Reactivity

Human

Applications

Knockout Validated, Western Blot, ELISA, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1021408
View all Caspase-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Caspase-1
Asn120-Asp297
Accession # P29466

Specificity

Detects human Caspase-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Caspase‑1 Antibody

Detection of Human Caspase‑1 antibody by Western Blot.

Detection of Human Caspase‑1 by Western Blot.

Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62153) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Caspase‑1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Caspase‑1 in U937 Human Cell Line.

Caspase‑1 was detected in immersion fixed U937 human histiocytic lymphoma cell line (positive) and MCF‑7 human breast cancer cell line (negative control) using Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62153) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human Caspase‑1 antibody by Simple WesternTM.

Detection of Human Caspase‑1 by Simple WesternTM.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Caspase‑1 at approximately 52 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62153). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human Caspase‑1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Caspase‑1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Caspase‑1 knockout A431 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62153) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Caspase‑1 at approximately 48 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Human Caspase‑1 ELISA Standard Curve.

Recombinant Human Caspase‑1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Caspase‑1 Monoclonal Antibody (MAB62151) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62153) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human Caspase‑1 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62151).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Caspase-1/ICE Quantikine ELISA Kit (Catalog # DCA100) for  a complete optimized ELISA.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Knockout Validated

Caspase‑1 is specifically detected in A431 human epithelial carcinoma cell line parental cell line but is not detectable in Caspase‑1 knockout A431 cell line.

Simple Western

20 µg/mL
Sample: A431 human epithelial carcinoma cell line

Western Blot

2 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line and A431 human epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB62153 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caspase-1

Caspase-1, also known as IL-1 beta -Converting Enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.

Alternate Names

CASP1, Caspase1, ICE

Entrez Gene IDs

834 (Human)

Gene Symbol

CASP1

UniProt

P29466 (Human)

Additional Caspase-1 Products

Product Documents for Human Caspase‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Caspase‑1 Antibody

For research use only

Customer Reviews for Human Caspase‑1 Antibody (1)

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  • Human Caspase-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: A431 human epithelial carcinoma cell line
    Species: Human
    Verified Customer | Posted 03/07/2022
    Human Caspase‑1 Antibody MAB62153

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Protocols

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