Human CCL4/MIP-1 beta Antibody (24006)

R&D Systems | Catalog # MAB271

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Antibody Array Development, ELISA Development, ELISpot Development, Immunodepletion, Lateral Flow Assay, Luminex Development, CyTOF-reported

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 24006
View all CCL4/MIP-1 beta Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
Ala24-Asn92
Accession # P13236

Specificity

Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, less than 4% cross-reactivity with recombinant human(rh) CCL3/MIP‑1 alpha and no cross-reactivity with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL4/MIP-1 beta Antibody (24006)

Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Human CCL4/MIP‑1 beta Antibody.

Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Human CCL4/MIP‑1 beta Antibody.

Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL4/MIP-1 beta (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL4/MIP-1 beta Monoclonal Antibody (Catalog # MAB271). The ND50 is typically 0.3-1.2 µg/mL.
Detection of CCL4/MIP-1 beta antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monoctyes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP-1 beta Monoclonal Antibody (Catalog # MAB271) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Human CCL4 / MIP-1 beta ELISA Standard Curve

Human CCL4 / MIP-1 beta ELISA Standard Curve

Recombinant Human CCL4/MIP‑1 beta (Catalog # 271-BME) was serially diluted and captured by Mouse Anti-Human CCL4/MIP‑1 beta Monoclonal Antibody (Catalog # MAB271) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL4/MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-271-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL4/MIP-1 beta Antibody (24006)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood monoctyes treated with LPS and Monensin were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

1 µg/mL
Sample: Recombinant Human CCL4/MIP‑1 beta (Catalog # 271-BME)

Neutralization

Measured by its ability to neutralize CCL4/MIP‑1 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 0.04 µg/mL Recombinant Human CCL4/MIP‑1 beta.

Human CCL4/MIP-1 beta Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CCL4/MIP‑1 beta Biotinylated Antibody (Catalog # BAF271)
  • Standard: Recombinant Human CCL4/MIP-1 beta Protein (Catalog # 271-BME)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 5 using MAB271 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL4/MIP-1 beta

CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

Alternate Names

Exodus-3, MIP-1 beta, MIP1 beta

Entrez Gene IDs

6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine); 102117861 (Cynomolgus Monkey)

Gene Symbol

CCL4

UniProt

P13236

Additional CCL4/MIP-1 beta Products

Product Documents for Human CCL4/MIP-1 beta Antibody (24006)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL4/MIP-1 beta Antibody (24006)

For research use only

Citations for Human CCL4/MIP-1 beta Antibody (24006)

Customer Reviews for Human CCL4/MIP-1 beta Antibody (24006) (5)

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Human CCL4/MIP-1 beta MAb (Clone 24006)
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Primate
    Verified Customer | Posted 11/28/2022
    Worked as a capture IgG for the NHP ELISA
    Human CCL4/MIP-1 beta Antibody (24006) MAB271
  • Human CCL4/MIP-1 beta Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 08/20/2021
    Human CCL4/MIP-1 beta Antibody (24006) MAB271
  • Human CCL4/MIP-1 beta Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 08/19/2019
    Human CCL4/MIP-1 beta Antibody (24006) MAB271
  • Human CCL4/MIP-1 beta Antibody
    Name: Michelle Chen
    Application: Block/Neutralize
    Sample Tested: Peripheral blood neutrophils
    Species: Human
    Verified Customer | Posted 04/26/2017
    Neutrophil derived CCL4 was neutralized in co-culture with A375 tumor cells. This effect on the extravasation of A375 MA2 cells was assessed.
    Human CCL4/MIP-1 beta Antibody (24006) MAB271
  • Human CCL4/MIP‑1 beta Antibody
    Name: Anonymous
    Application: Affinity Purification
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Dengue virus
    Verified Customer | Posted 04/25/2016

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