Human CD25/IL-2 R alpha Antibody

  • Specificity
    Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) IL‑2 sR gamma is observed, less than 5% cross-reactivity with rhIL-6 sR is observed, and less than 1% cross-reactivity with rhIL-1 sRII and rhIL-2 sR beta is observed. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-2 R alpha is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
    Accession # P01589
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
  • Western Blot
    0.1 µg/mL
    Recombinant Human CD25/IL‑2 R alpha (Catalog # 223-2A)
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood lymphocytes
  • Neutralization
    Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL-2 and Neutralization by Human CD25/IL-2 Ra Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA). The ND50 is typically 0.2‑1 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2 R alpha
Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.
  • Long Name:
    Interleukin 2 Receptor alpha
  • Entrez Gene IDs:
    3559(Human); 16184(Mouse); 25704(Rat); 403870(Canine)
  • Alternate Names:
    CD25; IL-2 R alpha; IL2RA; CD25; CD25 antigen; IDDM10; IL-2 receptor subunit alpha; IL2R; IL-2R subunit alpha; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab.
    Authors: Binder M, Vogtle FN, Michelfelder S, Muller F, Illerhaus G, Sundararajan S, Mertelsmann R, Trepel M
    Cancer Res., 2007;67(8):3518-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Cytometry,
  2. Interleukin-2alpha receptor in membrane lipid rafts.
    Authors: Li QR, Ma J, Wang H, Li JS
    Transplant. Proc., 2005;37(5):2395-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: Western Blot,
  3. Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses.
    Authors: Sandrin V, Muriaux D, Darlix JL, Cosset FL
    J. Virol., 2004;78(13):7153-64.
    Species: Human
    Sample Type: Cell Lysates
    Application: Western Blot,
  4. Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
    Authors: Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
    J. Immunol., 2008;180(11):7221-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: Western Blot,


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