Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 15% cross-reactivity with recombinant human (rh) IL‑2 sR gamma is observed, less than 5% cross-reactivity with rhIL-6 sR is observed, and less than 1% cross-reactivity with rhIL-1 sRII and rhIL-2 sR beta is observed. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-2 R alpha is observed.
Polyclonal Goat IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha Glu22-Cys213 Accession # P01589
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human CD25/IL‑2 R alpha (Catalog # 223-2A)
2.5 µg/106 cells
Human whole blood lymphocytes
Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are
available in the Technical Information section on our website.
Cell Proliferation Induced by IL-2 and Neutralization by Human CD25/IL-2 Ra Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA). The ND50 is typically 0.2‑1 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2 R alpha
Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.