Quick Order  

Welcome to Bio-Techne Quick Order!

Please enter a catalog number:
 
Enter CSV values, as SKU, QUANTITY.
 
View Your Cart
Simplified Shipping

We believe shipping should be simple. We charge a flat rate that depends on your currency and location.
  • No hidden charges
  • No additional charges for dry ice
  • No additional charges for multiple shipments
Choose a shipping destination
Albania

Algeria

Andorra

Angola

Antigua and Barbuda

Argentina

Armenia

Aruba

Australia

Austria

Azerbaijan

Bahamas

Bahrain

Bangladesh

Barbados

Belarus

Belgium

Belize

Benin

Bermuda

Bolivia

Bosnia and Herzegovina

Botswana

Brazil

Brunei

Bulgaria

Burkina Faso

Burundi

Cambodia

Cameroon

Canada

Cayman Islands

Central African Republic

Chad

Chile

China

Colombia

Costa Rica

Ivory Coast

Croatia

Cyprus

Czech Republic

Denmark

Djibouti

Dominica

Dominican Republic

Ecuador

Egypt

El Salvador

Equatorial Guinea

Eritrea

Estonia

Ethiopia

Falkland Islands

Fiji

Finland

France

French Polynesia

Gabon

Gambia

Georgia

Germany

Ghana

Gibraltar

Greece

Grenada

Guadeloupe

Guam

Guatemala

Guinea

Guinea-Bissau

Guyana

Haiti

Vatican

Honduras

Hong Kong S.A.R., China

Hungary

Iceland

India

Indonesia

Iraq

Ireland

Israel

Italy

Jamaica

Japan

Jordan

Kazakhstan

Kenya

South Korea

Kuwait

Kyrgyzstan

Latvia

Lebanon

Lesotho

Liberia

Liechtenstein

Lithuania

Luxembourg

Macedonia

Madagascar

Malawi

Malaysia

Mali

Malta

Marshall Islands

Mauritania

Mexico

Moldova

Monaco

Mongolia

Montenegro

Morocco

Mozambique

Myanmar

Namibia

Nepal

Netherlands

Netherlands Antilles

New Caledonia

New Zealand

Nicaragua

Niger

Nigeria

Northern Mariana Islands

Norway

Oman

Pakistan

Palau

Panama

Papua New Guinea

Paraguay

Peru

Philippines

Poland

Portugal

Puerto Rico

Qatar

Romania

Russia

Rwanda

Saint Kitts and Nevis

Saint Lucia

Saint Martin (French part)

Samoa

Saudi Arabia

Senegal

Serbia

Sierra Leone

Singapore

Slovakia

Slovenia

Solomon Islands

South Africa

Spain

Sri Lanka

Suriname

Swaziland

Sweden

Switzerland

Taiwan

Tanzania

Thailand

Togo

Trinidad and Tobago

Tunisia

Turkey

Uganda

Ukraine

United Arab Emirates

United Kingdom

United States

Uruguay

Uzbekistan

Venezuela

Vietnam

Yemen

Zambia

Zimbabwe

 

OR

Human CD25/IL-2 R alpha Antibody

  • Specificity
    Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) IL‑2 sR gamma is observed, less than 5% cross-reactivity with rhIL-6 sR is observed, and less than 1% cross-reactivity with rhIL-1 sRII and rhIL-2 sR beta is observed. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-2 R alpha is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
    Glu22-Cys213
    Accession # P01589
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human CD25/IL‑2 R alpha (Catalog # 223-2A)
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood lymphocytes
  • Neutralization
    Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL-2 and Neutralization by Human CD25/IL-2 Ra Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA). The ND50 is typically 0.2‑1 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2 R alpha
Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.
  • Long Name:
    Interleukin 2 Receptor alpha
  • Entrez Gene IDs:
    3559 (Human); 25704 (Human); 16184 (Human); 403870 (Canine);
  • Alternate Names:
    CD25; IL-2 R alpha; IL2RA; CD25; CD25 antigen; IDDM10; IL-2 receptor subunit alpha; IL2R; IL-2R subunit alpha; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 4 of 4
Filter your results:

Applications
Sample Type
  1. Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab.
    Authors: Binder M, Vogtle FN, Michelfelder S, Muller F, Illerhaus G, Sundararajan S, Mertelsmann R, Trepel M
    Cancer Res., 2007;67(8):3518-23.
    Species: Human
    Application: Flow Cytometry,
  2. Interleukin-2alpha receptor in membrane lipid rafts.
    Authors: Li QR, Ma J, Wang H, Li JS
    Transplant. Proc., 2005;37(5):2395-7.
    Species: Human
    Application: Western Blot,
  3. Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses.
    Authors: Sandrin V, Muriaux D, Darlix JL, Cosset FL
    J. Virol., 2004;78(13):7153-64.
  4. Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
    Authors: Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
    J. Immunol., 2008;180(11):7221-9.
    Species: Human
    Application: Western Blot,

FAQs

No product specific FAQs exist for this product, however you may click here to view all FAQs for Primary Antibodies

Order Details

Contact Information

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest

Feedback

Customer Information