Human CD25/IL-2 R alpha Antibody

Name: Human CD25/IL-2 R alpha Antibody
Brand: R&D Systems
Rating: 4 (1 reviews)

(4 citations)

(1 Review)

Datasheet / CoA / SDS

Neutralization

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Product Details

Citations (4)

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Species Reactivity

Human
Specificity

Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) IL‑2 sR gamma is observed, less than 5% cross-reactivity with rhIL-6 sR is observed, and less than 1% cross-reactivity with rhIL-1 sRII and rhIL-2 sR beta is observed. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-2 R alpha is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
Glu22-Cys213
Accession # P01589
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CD25/IL‑2 R alpha (Catalog # 223-2A)
Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood lymphocytes
CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND₅₀) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization

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Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody. Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL‑2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA). The ND₅₀ is typically 0.2‑1 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2 R alpha

Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

Long Name:

Interleukin 2 Receptor alpha

Entrez Gene IDs:

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine)

Alternate Names:

CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species	Applications	Sample Type
Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients. Authors: Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH J. Immunol., 2008;180(11):7221-9. Species: Human Sample Type: Cell Lysates Application: WB Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab. Authors: Binder M, Vogtle FN, Michelfelder S, Muller F, Illerhaus G, Sundararajan S, Mertelsmann R, Trepel M Cancer Res., 2007;67(8):3518-23. Species: Human Sample Type: Whole Cells Application: Flow Interleukin-2alpha receptor in membrane lipid rafts. Authors: Li QR, Ma J, Wang H, Li JS Transplant. Proc., 2005;37(5):2395-7. Species: Human Sample Type: Cell Lysates Application: WB Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses. Authors: Sandrin V, Muriaux D, Darlix JL, Cosset FL J. Virol., 2004;78(13):7153-64. Species: Human Sample Type: Cell Lysates Application: WB

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Isotype Controls

Description Application Cat# Citations Images

Normal Goat IgG Control

Ctrl AB-108-C 200

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Human CD25/IL-2 R alpha Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

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Chicken Anti-Goat IgG Biotinylated Antibody

Rabbit Anti-Goat IgG Biotinylated Antibody

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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