Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1057805
View all CGRP2 Primary Antibodies »

Product Specifications

Immunogen

Human CGRP synthetic peptide (Tocris (Catalog # 3012)
Accession # P10092

Specificity

Detects Human Procalcitonin in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CGRP2 Antibody

Detection of Human CGRP2 by Western Blot.

Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CGRP2 Monoclonal Antibody (Catalog # MAB11382) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CGRP2 at approximately ~9 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Human CGRP2 ELISA Standard Curve.

Recombinant Human CGRP2 protein was serially diluted 2-fold and captured by Mouse Anti-Human CGRP2 Monoclonal Antibody (Catalog # MAB11382) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Procalcitonin Monoclonal Antibody (MAB11323) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Detection of CGRP2 in TT cells (positive) and THP‑1 cells (negative).

CGRP2 was detected in immersion fixed TT human medullary thyroid cancer cell line (positive) and absent in THP‑1 human acute monocytic leukemia cell line (negative) using Mouse Anti-Human CGRP2 Monoclonal Antibody (Catalog # MAB11382) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CGRP2 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB11323).This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed TT human medullary thyroid cancer cell line (positive) and THP‑1 human acute monocytic leukemia cell line (negative)

Western Blot

1 µg/mL
Sample: TT human medullary thyroid cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CGRP2

Calcitonin gene-related peptide or CGRP is a 37-amino acid neuropeptide formed by alternative splicing of the calcitonin/CGRP gene. CGRP is a member of the calcitonin family of peptides consisting of calcitonin, amylin, adrenomedullin, adrenomedullin 2 (intermedin) and calcitonin‑receptor‑stimulating peptide. CGRP is produced in both peripheral and central neurons and induces vasodilation of a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.

Long Name

Calcitonin Related Polypeptide Beta

Alternate Names

beta-CGRP, CALC2, Calcitonin 2, CGRP-II, FLJ30166

Entrez Gene IDs

797 (Human)

Gene Symbol

CALCB

UniProt

P10092

Additional CGRP2 Products

Product Documents for Human CGRP2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CGRP2 Antibody

For research use only

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Protocols

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