Human FRA‑1 Antibody

Name: Human FRA‑1 Antibody
Brand: R&D Systems
SKU: MAB4935
Price: 149 USD
Availability: InStock

R&D Systems | Catalog # MAB4935

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
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Specific Notices
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 496106

View all FRA-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FRA-1
Gly146-Ala247
Accession # P15407

Specificity

Detects human FRA-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FRA-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human FRA‑1 Antibody

Detection of Human FRA‑1 by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 18 hours. PVDF Membrane was probed with 1 µg/mL of Human FRA-1 Monoclonal Antibody (Catalog # MAB4935) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRA-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FRA‑1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: A172 human glioblastoma cell line treated with Recombiant Human EGF (Catalog # 236-EG)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: FRA-1

FRA-1 (FOSL1) is a 271 amino acid member of the leucine zipper Fos family of transcription factors. Fos family members have been associated with cellular proliferation, differentiation and transformation. In accordance with these findings, overexpression of FRA-1 correlates with a malignant phenotype in breast cancer. In lung epithelia cells, FRA-1 promotes motility, invasion, and anchorage independent growth. FRA-1 knock out mice rescued for the FRA-1 placental defect are viable but show reduced bone formation that may be due to a defect in the osteoblast differentiation. Human FRA-1 and mouse FRA-1 are 94% identical between amino acids 146-247.