Human IL‑1 beta /IL‑1F2 Antibody

R&D Systems | Catalog # AB-201-NA

R&D Systems
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Porcine

Applications

Validated:

Western Blot, Neutralization, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-1 beta /IL-1F2
Ala117-Ser269
Accession # NP_000567

Specificity

Detects human IL‑1 beta /IL‑1F2 in ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant rhesus monkey is observed, and approximately 50% cross-reactivity with recombinant mouse IL‑1 beta, recombinant rat IL‑1 beta, recombinant canine IL‑1 beta, recombinant equine IL‑1 beta, recombinant feline IL‑1 beta, and approximately 20% cross-reactivity with recombinant porcine IL-1 beta and recombinant cotton rat IL-1 beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL‑1 beta /IL‑1F2 Antibody

Detection of Human and Mouse IL-1 beta /IL-1F2 antibody by Western Blot.

Detection of Human and Mouse IL‑1 beta /IL‑1F2 by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IL-1 beta /IL-1F2 antibody in Human Peripheral Blood Mononuclear Cells by Immunocytochemistry (ICC).

IL‑1 beta /IL‑1F2 in Human Peripheral Blood Mononuclear Cells.

IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells using Goat Anti-Human IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-201-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Human IL‑1 beta / IL‑1F2 Antibody.

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Human IL‑1 beta / IL‑1F2 Antibody.

Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta / IL-1F2 (50 pg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human IL-1 beta /IL-1F2 Poly-clonal Antibody (Catalog # AB-201-NA). The ND50 is typically 0.05-0.1 µg/mL.

Applications for Human IL‑1 beta /IL‑1F2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells

Western Blot

1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line -/+ PMA/LPS and RAW 264.7 mouse monocyte/macrophage cell line -/+ LPS.

Neutralization

Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.05-0.1 µg/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2.

Reviewed Applications

Read 1 review rated 5 using AB-201-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

NP_000567

Additional IL-1 beta/IL-1F2 Products

Product Documents for Human IL‑1 beta /IL‑1F2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑1 beta /IL‑1F2 Antibody

This product is covered by one or more of the following U.S. patents: 4,766,069, 5,510,462, 5,681,933, 4,762,914, 5,474,899, 5,789,185, 5,484,887, 5,122,459, 5,001,057, 5,077,219, 5,286,847.

For research use only

Citations for Human IL‑1 beta /IL‑1F2 Antibody

Customer Reviews for Human IL‑1 beta /IL‑1F2 Antibody (1)

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  • Human IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 09/02/2022
    Human IL‑1 beta /IL‑1F2 Antibody AB-201-NA

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