hh Human IL-4 R alpha Antibody MAB230-100: R&D Systems

Human IL-4 R alpha Antibody

(17 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL‑4 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-9 R, recombinant mouse IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-13 R alpha 1, or rhIL-13 R alpha 2 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 25463
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R
    Gly24-His232
    Accession # P24394
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IL‑4 R alpha (Catalog # 230-4R) under non-reducing conditions only
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 3-6 ng/mL in the presence of 0.2 ng/mL Recombinant Human IL‑4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑4 R alpha in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL‑4 R alpha Monoclonal Antibody (Catalog # MAB230, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunohistochemistry
IL‑4 R alpha in Human Lymph Node. IL‑4 R alpha was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human IL‑4 R alpha Monoclonal Antibody (Catalog # MAB230) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha  Antibody. Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑4 R alpha Monoclonal Antibody (Catalog # MAB230). The ND50 is typically 3-6 ng/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-4 R alpha

Interleukin 4 receptor (IL-4 R) is the transmembrane ligand binding subunit of the heterodimeric IL-4 receptor complex. In the type I receptor complex, IL-4 R associates with the common gamma chain, whereas in the type II receptor complex, it associates with IL-13 R alpha 1. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.

  • Long Name:
    Interleukin 4 Receptor alpha
  • Entrez Gene IDs:
    3566 (Human); 16190 (Mouse)
  • Alternate Names:
    CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. IL-4 primes the dynamics of breast cancer progression via DUSP4 inhibition
    Authors: M Gaggianesi, A Turdo, A Chinnici, E Lipari, T Apuzzo, A Benfante, I Sperduti, S Di Franco, S Meraviglia, E Lo Presti, F Dieli, V Caputo, G Militello, S Vieni, G Stassi, M Todaro
    Cancer Res., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells.
    Authors: Reece P, Gauvreau G, Sehmi R, Denburg J
    PLoS ONE, 2014;9(6):e100734.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
    Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
    PLoS ONE, 2014;9(5):e96925.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Absence of a role for interleukin-13 in inflammatory bowel disease.
    Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
    Eur J Immunol, 2014;44(2):370-85.
  5. Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4.
    Authors: Volonte A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C
    J Immunol, 2014;192(1):523-32.
    Species: Human
    Sample Type: Whole Cells
    Application: IF
  6. Platelet ITAM signaling is critical for vascular integrity in inflammation.
    Authors: Boulaftali, Yacine, Hess, Paul R, Getz, Todd M, Cholka, Agnieszk, Stolla, Moritz, Mackman, Nigel, Owens, A Philli, Ware, Jerry, Kahn, Mark L, Bergmeier, Wolfgang
    J Clin Invest, 2013;123(2):908-16.
    Species: Human
    Sample Type: Whole Cells
    Application: Binding Assay
  7. Construction and application of elastin like polypeptide containing IL-4 receptor targeting peptide.
    Authors: Sarangthem, Vijaya, Cho, Eun A, Bae, Sang Mun, Singh, Thoudam, Kim, Sun-Ji, Kim, Soyoun, Jeon, Won Bae, Lee, Byung-He, Park, Rang-Woo
    PLoS ONE, 2013;8(12):e81891.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  8. Hrs Recognizes a Hydrophobic Amino Acid Cluster in Cytokine Receptors during Ubiquitin-independent Endosomal Sorting.
    Authors: Amano Y, Yamashita Y, Kojima K, Yoshino K, Tanaka N, Sugamura K, Takeshita T
    J. Biol. Chem., 2011;286(17):15458-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Fatty acid amide hydrolase in prostate cancer: association with disease severity and outcome, CB1 receptor expression and regulation by IL-4.
    Authors: Thors L, Bergh A, Persson E, Hammarsten P, Stattin P, Egevad L, Granfors T, Fowler CJ
    PLoS ONE, 2010;5(8):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function.
    Authors: Hu A, Fatma S, Cao J, Grunstein JS, Nino G, Grumbach Y, Grunstein MM
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L790-803.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. Interleukin-4 induces the activation and collagen production of cultured human intrahepatic fibroblasts via the STAT-6 pathway.
    Authors: Aoudjehane L, Pissaia A, Scatton O, Podevin P, Massault PP, Chouzenoux S, Soubrane O, Calmus Y, Conti F
    Lab. Invest., 2008;88(9):973-85.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway.
    Authors: Aoudjehane L, Podevin P, Scatton O, Jaffray P, Dusanter-Fourt I, Feldmann G, Massault PP, Grira L, Bringuier A, Dousset B, Chouzenoux S, Soubrane O, Calmus Y, Conti F
    FASEB J., 2007;21(7):1433-44.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
    Authors: Hirst SJ, Hallsworth MP, Peng Q
    Am. J. Respir. Crit. Care Med., 2002;165(8):1161-71.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
    Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
    Eur. J. Immunol., 2001;31(4):1037-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  17. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001;70(4):617-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 17 Citations
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Average Rating: 4 (Based on 1 review)

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Block/Neutralize Human IL-4 R alpha  Antibody MAB230
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 Block/Neutralize Human Anonymous 12/06/2017
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Block/Neutralize Human IL-4 R alpha  Antibody MAB230
Block/Neutralize: Human IL-4 R alpha Antibody [MAB230]

Summary

ApplicationBlock/Neutralize
Sample TestedRecombinant protein
SpeciesHuman

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