Human LRP-1 Intracellular Domain Antibody

Catalog # Availability Size / Price Qty
MAB6360
MAB6360-SP
Detection of Human LRP-1 by Western Blot.
3 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Human LRP-1 Intracellular Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRP-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) LRP‑1 clusters 2, 3, or 4, rhLRP‑4, rhLRP-5, rhLRP-5 intracellular domain, rhLRP-6, rhLRP-6 intracellular domain, or rhLRPAP is observed.
Source
Monoclonal Mouse IgG2B Clone # 545503
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human LRP-1
Gln4449-ala4544
Accession # Q07954
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human LRP-1 antibody by Western Blot. View Larger

Detection of Human LRP-1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human LRP-1 Intracellular Domain Monoclonal Antibody (Catalog # MAB6360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRP-1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry LRP-1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

LRP‑1 in HepG2 Human Cell Line. LRP-1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human LRP-1 Intracellular Domain Monoclonal Antibody (Catalog # MAB6360) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry LRP-1 antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

LRP‑1 in Human Liver. LRP-1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human LRP-1 Intracellular Domain Monoclonal Antibody (Catalog # MAB6360) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-1

LRP-1 (low-density lipoprotein receptor-related protein 1) is a large type I transmembrane protein belonging to the LDL receptor superfamily. The extracellular domain of LRP-1 is organized in four clusters of ligand-binding repeats that recognizes at least 30 different ligands. The amino acid sequence of human and mouse LRP-1 cluster II share 100% identity. LRP-1 is an endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. It plays a crucial role in early embryonic development.

Long Name
LDL Receptor-related Protein 1
Entrez Gene IDs
4035 (Human)
Alternate Names
A2MR; Alpha-2-Macroglobulin Receptor; APOER; Apolipoprotein E receptor; APR; CD91 antigen; CD91; EC 1.1.1.94; EC 3.4.21.9; IGFBP3R; low density lipoprotein receptor-related protein 1; LRP; LRP-1; LRP1A; prolow-density lipoprotein receptor-related protein 1; TbetaR-V/LRP-1/IGFBP-3 receptor; TGFBR5; type V tgf-beta receptor

Product Datasheets

You must select a language.

x

Citations for Human LRP-1 Intracellular Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. APOE2 mitigates disease-related phenotypes in an isogenic hiPSC-based model of Alzheimer's disease
    Authors: N Brookhouse, S Raman, C Frisch, G Srinivasan, DA Brafman
    Molecular Psychiatry, 2021-04-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Isocitrate dehydrogenase 1 mutation enhances 24(S)-hydroxycholesterol production and alters cholesterol homeostasis in glioma
    Authors: R Yang, Y Zhao, Y Gu, Y Yang, X Gao, Y Yuan, L Xiao, J Zhang, C Sun, H Yang, J Qin, J Li, F Zhang, L Zhang, J Ye
    Oncogene, 2020-08-27;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. A Potential Neuroprotective Role of Apolipoprotein E-containing Lipoproteins through Low Density Lipoprotein Receptor-related Protein 1 in Normal Tension Glaucoma.
    Authors: Hayashi H, Eguchi Y, Fukuchi-Nakaishi Y, Takeya M, Nakagata N, Tanaka K, Vance JE, Tanihara H
    J. Biol. Chem., 2012-06-06;287(30):25395-406.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human LRP-1 Intracellular Domain Antibody

There are currently no reviews for this product. Be the first to review Human LRP-1 Intracellular Domain Antibody and earn rewards!

Have you used Human LRP-1 Intracellular Domain Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review