Human LRP-1 Intracellular Domain Antibody Summary
Accession # Q07954
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human LRP-1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human LRP-1 Intracellular Domain Monoclonal Antibody (Catalog # MAB6360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRP-1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LRP‑1 in HepG2 Human Cell Line. LRP-1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human LRP-1 Intracellular Domain Monoclonal Antibody (Catalog # MAB6360) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
LRP‑1 in Human Liver. LRP-1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human LRP-1 Intracellular Domain Monoclonal Antibody (Catalog # MAB6360) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
LRP-1 (low-density lipoprotein receptor-related protein 1) is a large type I transmembrane protein belonging to the LDL receptor superfamily. The extracellular domain of LRP-1 is organized in four clusters of ligand-binding repeats that recognizes at least 30 different ligands. The amino acid sequence of human and mouse LRP-1 cluster II share 100% identity. LRP-1 is an endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. It plays a crucial role in early embryonic development.
Citations for Human LRP-1 Intracellular Domain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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APOE2 mitigates disease-related phenotypes in an isogenic hiPSC-based model of Alzheimer's disease
Authors: N Brookhouse, S Raman, C Frisch, G Srinivasan, DA Brafman
Molecular Psychiatry, 2021;0(0):.
Sample Types: Whole Cells
Applications: Flow Cytometry
Isocitrate dehydrogenase 1 mutation enhances 24(S)-hydroxycholesterol production and alters cholesterol homeostasis in glioma
Authors: R Yang, Y Zhao, Y Gu, Y Yang, X Gao, Y Yuan, L Xiao, J Zhang, C Sun, H Yang, J Qin, J Li, F Zhang, L Zhang, J Ye
Sample Types: Tissue Homogenates
Applications: Western Blot
A Potential Neuroprotective Role of Apolipoprotein E-containing Lipoproteins through Low Density Lipoprotein Receptor-related Protein 1 in Normal Tension Glaucoma.
Authors: Hayashi H, Eguchi Y, Fukuchi-Nakaishi Y, Takeya M, Nakagata N, Tanaka K, Vance JE, Tanihara H
J. Biol. Chem., 2012;287(30):25395-406.
Sample Types: Cell Lysates
Applications: Western Blot
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