Human/Mouse CD117/c-kit Antibody

  (25 citations)
(4 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (25)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse CD117/c-kit in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD117 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
    Gln25-Thr519 (Ala207Glu)
    Accession # P05532
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Note: Certificate of Analysis not available for kit components.

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Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    Lineage depleted mouse bone marrow cells
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CD117/c-kit in Mouse Embryo. CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human and Mouse CD117/c-kit by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse CD117/c-kit by Simple WesternTM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells. 

  • Long Name:
    Stem Cell Factor Receptor
  • Entrez Gene IDs:
    3815 (Human); 16590 (Mouse); 64030 (Rat)
  • Alternate Names:
    CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis
    Authors: Y Kawai, A Oda, Y Kanai, R Goitsuka
    PLoS ONE, 2018;13(1):e0190702.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-P
  3. Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis
    Authors: S Saeidi, F Shapouri, RU de Iongh, F Casagranda, JM Sutherland, PS Western, EA McLaughlin, M Familari, GR Hime
    PLoS ONE, 2018;13(1):e0190925.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-P
  4. Cardiac Nestin+ Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells
    Authors: D Xie, Y Liao, B Wu, Y Chen, W Lin, D Lu, S Gao, S Zhu, C Peng, MH Jiang
    Int Heart J, 2018;59(1):180-189.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  5. Concurrent Isolation of Three Distinct Cardiac Stem Cell Populations from a Single Human Heart Biopsy
    Authors: MM Monsanto, KS White, T Kim, BJ Wang, K Fisher, K Ilves, FG Khalafalla, A Casillas, K Broughton, S Mohsin, WP Dembitsky, MA Sussman
    Circ. Res., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures
    Authors: MY Lee, SE Ha, C Park, PJ Park, R Fuchs, L Wei, BG Jorgensen, D Redelman, SM Ward, KM Sanders, S Ro
    PLoS ONE, 2017;12(4):e0176031.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Fabrication of Synthetic Mesenchymal Stem Cells for the Treatment of Acute Myocardial Infarction in Mice
    Authors: L Luo, J Tang, K Nishi, C Yan, PU Dinh, J Cores, T Kudo, J Zhang, TS Li, K Cheng
    Circ. Res, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  8. Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes
    Authors: AL Feng, YY Xiang, L Gui, G Kaltsidis, Q Feng, WY Lu
    Diabetologia, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  9. Estrogen induces c-Kit and an aggressive phenotype in a model of invasive lobular breast cancer
    Authors: JC Harrell, TM Shroka, BM Jacobsen
    Oncogenesis, 2017;6(11):2.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Live cell screening platform identifies PPAR? as a regulator of cardiomyocyte proliferation and cardiac repair
    Authors: A Magadum, Y Ding, L He, T Kim, MD Vasudevara, Q Long, K Yang, N Wickramasi, HV Renikunta, N Dubois, G Weidinger, Q Yang, FB Engel
    Cell Res., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  11. Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice
    PLoS ONE, 2016;11(5):e0152179.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  12. Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growth.
    Authors: Shiu J, Piazuelo M, Ding H, Czinn S, Drakes M, Banerjee A, Basappa N, Kobayashi K, Fricke W, Blanchard T
    Mucosal Immunol, 2015;8(5):1047-59.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  13. Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis.
    Authors: Tanaka Y, Inoue-Yokoo T, Kulkeaw K, Yanagi-Mizuochi C, Shirasawa S, Nakanishi Y, Sugiyama D
    PLoS ONE, 2015;10(9):e0138621.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  14. Nuclear Calcium/Calmodulin-dependent Protein Kinase II Signaling Enhances Cardiac Progenitor Cell Survival and Cardiac Lineage Commitment.
    Authors: Quijada P, Hariharan N, Cubillo J, Bala K, Emathinger J, Wang B, Ormachea L, Bers D, Sussman M, Poizat C
    J Biol Chem, 2015;290(42):25411-26.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. Annexin A8 identifies a subpopulation of transiently quiescent c-kit positive luminal progenitor cells of the ductal mammary epithelium.
    Authors: Iglesias J, Cairney C, Ferrier R, McDonald L, Soady K, Kendrick H, Pringle M, Morgan R, Martin F, Smalley M, Blyth K, Stein T
    PLoS ONE, 2015;10(3):e0119718.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. c-kit+ cells minimally contribute cardiomyocytes to the heart.
    Authors: van Berlo J, Kanisicak O, Maillet M, Vagnozzi R, Karch J, Lin S, Middleton R, Marban E, Molkentin J
    Nature, 2014;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  17. Differential regulation of cellular senescence and differentiation by prolyl isomerase Pin1 in cardiac progenitor cells.
    Authors: Toko, Haruhiro, Hariharan, Nirmala, Konstandin, Mathias, Ormachea, Lucia, McGregor, Michael, Gude, Natalie, Sundararaman, Balaji, Joyo, Eri, Joyo, Anya Y, Collins, Brett, Din, Shabana, Mohsin, Sadia, Uchida, Takafumi, Sussman, Mark A
    J Biol Chem, 2014;289(9):5348-56.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  18. Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart.
    Authors: Smith A, Lewis F, Aquila I, Waring C, Nocera A, Agosti V, Nadal-Ginard B, Torella D, Ellison G
    Nat Protoc, 2014;9(7):1662-81.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC Fresh
  19. Thymosin beta4 increases the potency of transplanted mesenchymal stem cells for myocardial repair.
    Authors: Ye L, Zhang P, Duval S, Su L, Xiong Q, Zhang J
    Circulation, 2013;128(11):S32-41.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  20. Microenvironmental regulation of inducible nitric oxide synthase expression and nitric oxide production in mouse bone marrow-derived mast cells.
    Authors: Moon TC, Yoshimura T, Parsons T, Befus AD
    J. Leukoc. Biol., 2012;91(4):581-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  21. Generation of Functional Blood Vessels from a Single c-kit+ Adult Vascular Endothelial Stem Cell.
    Authors: Fang, Shentong, Wei, Jing, Pentinmikko, Nalle, Leinonen, Hannele, Salven, Petri
    PLoS Biol, 2012;10(10):e1001407.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  22. Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration.
    Authors: Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y
    Blood, 2012;119(23):5429-37.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  23. Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization.
    Authors: Miyamoto K, Yoshida S, Kawasumi M
    J. Exp. Med., 2011;208(11):2175-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  24. CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6.
    Authors: Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ
    Int. Immunol., 2009;21(8):991-1001.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  25. Harboring of particulate allergens within secretory compartments by mast cells following IgE/FcepsilonRI-lipid raft-mediated phagocytosis.
    Authors: Shin JS, Shelburne CP, Jin C, LeFurgey EA, Abraham SN
    J. Immunol., 2006;177(9):5791-800.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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Average Rating: 4.5 (Based on 4 reviews)

Have you used Human/Mouse CD117/c-kit Antibody?

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Read what people are saying who have used Human/Mouse CD117/c-kit Antibody.

We have 4 reviews tested in 3 species: Human, Mouse, Rat.
We have 4 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human/Mouse CD117/c-kit Antibody AF1356 Array AF1356
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Very Good
 ICC/IF Rat Anonymous 05/07/2018
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Image Details
Immunocytochemistry/Immunofluorescence Human/Mouse CD117/c-kit Antibody AF1356
Immunocytochemistry/Immunofluorescence: Human/Mouse CD117/c-kit Antibody [AF1356].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Liver tissue
Species Rat

Other Experimental Details

Other Experimental Details 1:100 dilutionRT overnight incubationCitrate buffer heat mediated antigen retrival
Western Blot Human/Mouse CD117/c-kit Antibody AF1356 Array AF1356
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Very Good
 WB Mouse Anonymous 04/05/2018
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Image Details
Western Blot Human/Mouse CD117/c-kit Antibody AF1356
Western Blot: Human/Mouse CD117/c-kit Antibody [AF1356].

Summary

Application Western Blot
Sample Tested ES-E14TG2a
Species Mouse

Other Experimental Details

Other Experimental Details The experiment was performed on ES-E14TG2a cells: two different bands of about 130 KDa (< 135 KDa) were detected.
  Excellent
 WB Human Anonymous 07/24/2017
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Summary

Application Western Blot
Sample Tested MCF-7 human breast cancer cell line,MCF 10A human breast epithelial cell line,MDA-MB-231 human breast cancer cell line
Species Human
Immunocytochemistry/Immunofluorescence Human/Mouse CD117/c-kit Antibody AF1356 Array AF1356
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Excellent
 ICC/IF Mouse Anonymous 08/08/2016
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Image Details
Immunocytochemistry/Immunofluorescence Human/Mouse CD117/c-kit Antibody AF1356
Immunocytochemistry/Immunofluorescence: Human/Mouse CD117/c-kit Antibody [AF1356].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Embryo tissue
Species Mouse

Other Experimental Details

Other Experimental Details Mouse embryonic yolk sac tissue (E10.5), with 1:100 dilution, Alex Fluro 594 donkey anti-goat 2nd antibody with 1:400 dilution

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