Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Porcine, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Dual RNAscope ISH-IHC Compatible, Simple Western, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD117/c-kit Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532

Specificity

Detects human and mouse CD117/c-kit in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse CD117/c-kit Antibody

CD117/c-kit antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

CD117/c-kit in Mouse Embryo.

CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human and Mouse CD117/c-kit antibody by Western Blot.

Detection of Human and Mouse CD117/c-kit by Western Blot.

Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9-2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human and Mouse CD117/c-kit antibody by Simple WesternTM.

Detection of Human and Mouse CD117/c-kit by Simple WesternTM.

Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse CD117/c-kit by Immunocytochemistry/Immunofluorescence

Detection of Mouse CD117/c-kit by Immunocytochemistry/Immunofluorescence

Localization of MAFB in mouse testes.(A) Localization of MAFB in E18.5 mouse testes. Double immunostaining of MAFB with E-cadherin (ECAD), GATA4, or STAR is shown. Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. G; germ cells. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells and Sertoli cells. (B) Localization of MAFB in adult mouse testes. Double immunostaining of MAFB with E-cadherin (ECAD), KIT, SCP3, PNA Lectin, or vimentin is shown. Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. All seminiferous tubules shown represent stage VII. US; undifferentiated spermatogonia. DS; differentiated spermatogonia. P; pachytene spermatocytes. Sp; spermatids. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells, Sertoli cells, and pachytene spermatocytes. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0190800), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse CD117/c-kit by Immunocytochemistry/Immunofluorescence

Detection of Mouse CD117/c-kit by Immunocytochemistry/Immunofluorescence

Spermatogenesis development of adult Mafb-cKO mice.Six-week-old mice were injected with tamoxifen and testes of cKO mice at 3- and 8-months of age were examined compared to those of age-matched controls (n = 4 for each group). (A) Testicular sections stained with Periodic acid-Schiff (PAS). (B) Immunostaining with various testicular cell types; undifferentiated spermatogonia (GFRA1 and PLZF), differentiated spermatogonia (KIT+ inside the tubule), spermatocytes (SCP3), spermatids (PNA-Lectin), Sertoli cells (SOX9 and Vimentin), and Leydig cells (KIT+ outside the tubule and STAR). The color of markers is indicated in the left boxes in panels. Colored arrows corresponding to the boxed markers are shown. (C) The expression level of the genes involved in testis function and germ cell development. The marker genes representing undifferentiated spermatogonia (Nanos3), differentiated spermatogonia (c-Kit), undifferentiated and differentiated spermatogonia (Sohlh1), differentiated spermatogonia and preleptotene spermatocytes (Stra8), spermatids (Prm2), Leydig cells (Hsd3b1), and Sertoli cells (Sox9) were analyzed by qRT-PCR. Each reaction was performed in duplicate for each gene. The data represent the means±SEM and are shown as relative mRNA expression after normalization to Hprt. *P<0.05. (D) The proportion of seminiferous stages: I-III, IV-VI, VII-VIII, and IX-XII from each genotype. *P<0.05. M; Month. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0190800), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse CD117/c-kit by Immunocytochemistry/Immunofluorescence

Detection of Mouse CD117/c-kit by Immunocytochemistry/Immunofluorescence

ANXA8+ve/c-kit+ve luminal progenitor cells are mostly Ki67−ve.Co-immunofluorescence staining for ANXA8 (green), c-kit (red), and Ki67 (purple) in mouse mammary gland from virgin and mid-pregnant mice shows that ANXA8+ve cells express c-kit, but not Ki67. The Ki67 staining has been coloured purple for easier visualisation in the triple-merged image. These are representative images of at least three independent mice for each time point. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25803307), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CD117/c-kit in Human Mouse Embryos.

Formalin-fixed paraffin-embedded tissue sections of mouse embryo were probed for CD117 mRNA (ACD RNAScope Probe, catalog # 314158; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-mouse CD117 polyclonal antibody (AF1356) at 5 μg/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in esophagus in mouse embryo.

Detection of Mouse CD117/c-kit by Flow Cytometry

Detection of Mouse CD117/c-kit by Flow Cytometry

c-kit-, F4/80- or Ly-6G-positive cell numbers in cornea after alkali injury. Corneal tissues were obtained 4 days after injury from vehicle- or SDF-1 alpha -treated BABL/c mice, and the tissues from 7 to 8 mice were combined and were subjected to analysis using a flow cytometer after being immunostained with anti-c-kit, anti-F4/80, or anti-Ly-6G antibody. Isotype IgG derived from the same species of the test antibody was used as negative control. Representative results from three to four tests of intracorneal infitlration of c-kit- (A), F4/80- (B), or Ly-6G-positive cells (C) from either vehicle- (left plot) or SDF-1 alpha -treated mice (right plot) are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/21850188), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CD117/c-kit in Human Melanoma

CD117/c-kit was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 0.5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse CD117/c-kit Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Dual RNAscope ISH-IHC Compatible

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human mouse embryos

Flow Cytometry

2.5 µg/106 cells
Sample: Lineage depleted mouse bone marrow cells

Immunohistochemistry

0.5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E15), paraffin-embedded human Melanoma

Simple Western

5 µg/mL
Sample: MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9&#x2011;2 mouse mast cell line

Western Blot

0.1 µg/mL
Sample: MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line

Reviewed Applications

Read 13 reviews rated 4 using AF1356 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells. 

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

UniProt

P05532

Additional CD117/c-kit Products

Product Documents for Human/Mouse CD117/c-kit Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse CD117/c-kit Antibody

For research use only

Citations for Human/Mouse CD117/c-kit Antibody

Customer Reviews for Human/Mouse CD117/c-kit Antibody (13)

4 out of 5
13 Customer Ratings
5 Stars
23%
4 Stars
69%
3 Stars
0%
2 Stars
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1 Stars
8%

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Customer Images

Showing  1 - 5 of 13 reviews Showing All
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  • Human/Mouse CD117/c-kit Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult small intestine
    Species: Human
    Verified Customer | Posted 10/16/2025
    Human ileum fixed in 4% PFA, cryopreserved in 30% sucrose and frozen. No specific staining in mast cells (tryptase+), which should express c-kit
    Human/Mouse CD117/c-kit Antibody AF1356
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Human/Mouse CD117/c-kit Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Nerve tissue
    Species: Mouse
    Verified Customer | Posted 10/26/2021
    +4°C overnight incubation 1:200, 2nd anti-goat 647 1:400
    Human/Mouse CD117/c-kit Antibody AF1356
  • Human/Mouse CD117/c-kit Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult intestine
    Species: Mouse
    Verified Customer | Posted 10/02/2019
    Human/Mouse CD117/c-kit Antibody AF1356
  • Human/Mouse CD117/c-kit Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Muscle tissue
    Species: Mouse
    Verified Customer | Posted 06/12/2019
  • Human/Mouse CD117/c-kit Antibody
    Name: Mozhdeh Sojoodi
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Liver tissue
    Species: Rat
    Verified Customer | Posted 05/07/2018
    1:100 dilution RT overnight incubation Citrate buffer heat mediated antigen retrival
    Human/Mouse CD117/c-kit Antibody AF1356
  • Human/Mouse CD117/c-kit Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: ES-E14TG2a
    Species: Mouse
    Verified Customer | Posted 04/05/2018
    The experiment was performed on ES-E14TG2a cells: two different bands of about 130 KDa (< 135 KDa) were detected.
    Human/Mouse CD117/c-kit Antibody AF1356
  • Human/Mouse CD117/c-kit Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse es cells
    Species: Mouse
    Verified Customer | Posted 03/23/2018
  • Human/Mouse CD117/c-kit Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 human breast cancer cell line, MCF 10A human breast epithelial cell line and MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 07/24/2017
  • Human/Mouse CD117/c-kit Antibody
    Name: Zhiheng He
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Embryo tissue
    Species: Mouse
    Verified Customer | Posted 08/08/2016
    Mouse embryonic yolk sac tissue (E10.5), with 1:100 dilution, Alex Fluro 594 donkey anti-goat 2nd antibody with 1:400 dilution
    Human/Mouse CD117/c-kit Antibody AF1356
  • Human/Mouse CD117/c-kit Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Brain (basal ganglia)
    Species: Mouse
    Verified Customer | Posted 03/22/2016
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 24375406
    Species: Mouse
    Verified Customer | Posted 01/05/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22262798
    Species: Mouse
    Verified Customer | Posted 01/05/2015
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: See PMID 24375406
    Species: Mouse
    Verified Customer | Posted 01/05/2015

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