Human/Mouse CD117/c-kit Antibody

(17 citations)
(2 Reviews)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse CD117/c-kit in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD117 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
    Gln25-Thr519 (Ala207Glu)
    Accession # P05532
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    Lineage depleted mouse bone marrow cells
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CD117/c-kit in Mouse Embryo. CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human and Mouse CD117/c-kit by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse CD117/c-kit by Simple WesternTM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells. 

  • Long Name:
    Stem Cell Factor Receptor
  • Entrez Gene IDs:
    3815 (Human); 16590 (Mouse); 64030 (Rat)
  • Alternate Names:
    CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Live cell screening platform identifies PPAR? as a regulator of cardiomyocyte proliferation and cardiac repair
    Authors: A Magadum, Y Ding, L He, T Kim, MD Vasudevara, Q Long, K Yang, N Wickramasi, HV Renikunta, N Dubois, G Weidinger, Q Yang, FB Engel
    Cell Res., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  2. Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures
    Authors: MY Lee, SE Ha, C Park, PJ Park, R Fuchs, L Wei, BG Jorgensen, D Redelman, SM Ward, KM Sanders, S Ro
    PLoS ONE, 2017;12(4):e0176031.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice
    PLoS ONE, 2016;11(5):e0152179.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  4. Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growth.
    Authors: Shiu J, Piazuelo M, Ding H, Czinn S, Drakes M, Banerjee A, Basappa N, Kobayashi K, Fricke W, Blanchard T
    Mucosal Immunol, 2015;8(5):1047-59.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  5. Annexin A8 identifies a subpopulation of transiently quiescent c-kit positive luminal progenitor cells of the ductal mammary epithelium.
    Authors: Iglesias J, Cairney C, Ferrier R, McDonald L, Soady K, Kendrick H, Pringle M, Morgan R, Martin F, Smalley M, Blyth K, Stein T
    PLoS ONE, 2015;10(3):e0119718.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. Nuclear Calcium/Calmodulin-dependent Protein Kinase II Signaling Enhances Cardiac Progenitor Cell Survival and Cardiac Lineage Commitment.
    Authors: Quijada P, Hariharan N, Cubillo J, Bala K, Emathinger J, Wang B, Ormachea L, Bers D, Sussman M, Poizat C
    J Biol Chem, 2015;290(42):25411-26.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis.
    Authors: Tanaka Y, Inoue-Yokoo T, Kulkeaw K, Yanagi-Mizuochi C, Shirasawa S, Nakanishi Y, Sugiyama D
    PLoS ONE, 2015;10(9):e0138621.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  8. Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart.
    Authors: Smith A, Lewis F, Aquila I, Waring C, Nocera A, Agosti V, Nadal-Ginard B, Torella D, Ellison G
    Nat Protoc, 2014;9(7):1662-81.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC Fresh
  9. c-kit+ cells minimally contribute cardiomyocytes to the heart.
    Authors: van Berlo J, Kanisicak O, Maillet M, Vagnozzi R, Karch J, Lin S, Middleton R, Marban E, Molkentin J
    Nature, 2014;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  10. Differential regulation of cellular senescence and differentiation by prolyl isomerase Pin1 in cardiac progenitor cells.
    Authors: Toko, Haruhiro, Hariharan, Nirmala, Konstandin, Mathias, Ormachea, Lucia, McGregor, Michael, Gude, Natalie, Sundararaman, Balaji, Joyo, Eri, Joyo, Anya Y, Collins, Brett, Din, Shabana, Mohsin, Sadia, Uchida, Takafumi, Sussman, Mark A
    J Biol Chem, 2014;289(9):5348-56.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  11. Thymosin beta4 increases the potency of transplanted mesenchymal stem cells for myocardial repair.
    Authors: Ye L, Zhang P, Duval S, Su L, Xiong Q, Zhang J
    Circulation, 2013;128(11):S32-41.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  12. Microenvironmental regulation of inducible nitric oxide synthase expression and nitric oxide production in mouse bone marrow-derived mast cells.
    Authors: Moon TC, Yoshimura T, Parsons T, Befus AD
    J. Leukoc. Biol., 2012;91(4):581-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  13. Generation of Functional Blood Vessels from a Single c-kit+ Adult Vascular Endothelial Stem Cell.
    Authors: Fang, Shentong, Wei, Jing, Pentinmikko, Nalle, Leinonen, Hannele, Salven, Petri
    PLoS Biol, 2012;10(10):e1001407.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  14. Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration.
    Authors: Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y
    Blood, 2012;119(23):5429-37.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  15. Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization.
    Authors: Miyamoto K, Yoshida S, Kawasumi M
    J. Exp. Med., 2011;208(11):2175-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  16. CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6.
    Authors: Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ
    Int. Immunol., 2009;21(8):991-1001.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  17. Harboring of particulate allergens within secretory compartments by mast cells following IgE/FcepsilonRI-lipid raft-mediated phagocytosis.
    Authors: Shin JS, Shelburne CP, Jin C, LeFurgey EA, Abraham SN
    J. Immunol., 2006;177(9):5791-800.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 17 Citations
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Average Rating: 5 (Based on 2 reviews)

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We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 applications: Western Blot, Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 WB Human Anonymous 07/24/2017
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Summary

ApplicationWestern Blot
Sample TestedMCF-7 human breast cancer cell line,MCF 10A human breast epithelial cell line,MDA-MB-231 human breast cancer cell line
SpeciesHuman
Immunocytochemistry/Immunofluorescence Human/Mouse CD117/c-kit Antibody AF1356
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Excellent
 ICC/IF Mouse Anonymous 08/08/2016
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Image Details
Immunocytochemistry/Immunofluorescence Human/Mouse CD117/c-kit Antibody AF1356
Immunocytochemistry/Immunofluorescence: Human/Mouse CD117/c-kit Antibody [AF1356]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedEmbryo tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsMouse embryonic yolk sac tissue (E10.5), with 1:100 dilution, Alex Fluro 594 donkey anti-goat 2nd antibody with 1:400 dilution

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