Human/Mouse/Rat Akt2 Antibody

Catalog # Availability Size / Price Qty
AF23151
AF23151-SP
Detection of Human/Mouse/Rat Akt2 by Western Blot.
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Human/Mouse/Rat Akt2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Akt2 in Western blots. Also detects recombinant human Akt2 but not recombinant Akt1 or Akt3 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt2
Accession # P31751
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat Akt2 by Western Blot. View Larger

Detection of Human/Mouse/Rat Akt2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat Akt2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF23151) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active Akt1 (Catalog # 1775-KS), recombinant human Akt2, and recombinant human Akt3 (5 ng/lane) were included. A specific band for Akt2 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Akt2  in Rat Testis. View Larger

Akt2 in Rat Testis. Akt2 was detected in perfusion fixed frozen sections of rat testis using Goat Anti-Human/Mouse/Rat Akt2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF23151) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry Akt2 in Human Kidney Cancer Tissue. View Larger

Akt2 in Human Kidney Cancer Tissue. Akt2 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 15 µg/mL Goat Anti-Human/Mouse/Rat Akt2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF23151) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Akt2  in Mouse Spleen. View Larger

Akt2 in Mouse Spleen. Akt2 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Human/Mouse/Rat Akt2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF23151) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection  of Human Akt2 by Simple WesternTM. View Larger

Detection of Human Akt2 by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Akt2 at approximately 60 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Akt2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF23151) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Akt2

The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). Akt2 is expressed predominantly in insulin target tissues such as liver, skeletal muscle, and fat. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.

Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog 2
Entrez Gene IDs
208 (Human); 11652 (Mouse); 29414 (Rat)
Alternate Names
Akt2; EC 2.7.11; EC 2.7.11.1; Murine thymoma viral (v-akt) homolog-2; PKB beta; PKBB; PKBBETA; PRKBB; Protein kinase Akt-2; Protein kinase B beta; rac protein kinase beta; RAC-beta serine/threonine-protein kinase; RAC-beta; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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