Human/Mouse/Rat eIF4E Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat eIF4E.
  • Source
    Monoclonal Mouse IgG2B Clone # 299910
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human eIF4E
    Met1-Val217
    Accession # P06730
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
eIF4E in MCF-7 Human Cell Line. eIF4E was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat eIF4E Monoclonal Antibody (Catalog # MAB3228) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human/Mouse/Rat eIF4E by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat eIF4E Monoclonal Antibody (Catalog # MAB3228) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for eIF4E at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eIF4E

eIF4E (eukaryotic initiation factor 4E) interacts with the 7-methyl-GTP cap structure to facilitate the initiation and rate of translation of mRNA. Together with eIF4G and eIF4A, it forms the eIF4F complex. eIF4E activity has been shown to play a role in cell cycle progression, tumorigenesis, embryonic development, nuclear export and synaptic plasticity.

  • Long Name:
    Eukaryotic Translation Initiation Factor 4E
  • Entrez Gene IDs:
    1977 (Human); 13684 (Mouse); 117045 (Rat)
  • Alternate Names:
    CBP; eIF4E; eIF-4E; EIF4E1; EIF4EL1; EIF4EL1MGC111573; eIF-4F 25 kDa subunit; EIF4F; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein
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Mouse IgG Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

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Mouse IgG2B Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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