Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 142102
View all p38 alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p38 alpha
Accession # Q16539

Specificity

Detects human, mouse, and rat p38 alpha. This antibody does not detect recombinant p38 beta, p38 gamma or p38 delta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat p38 alpha Antibody

Detection of Human/Mouse/Rat p38a antibody by Western Blot.

Detection of Human/Mouse/Rat p38 alpha by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat p38a Monoclonal Antibody (Catalog # MAB869) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p38a at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Western Blot Shows Human p38a Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human p38 alpha Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and p38a knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat p38a Monoclonal Antibody (Catalog # MAB869) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for p38a at approximately 38 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse p38 alpha by Western Blot

Detection of Mouse p38 alpha by Western Blot

Muscle-specific deletion of the p38 alpha, p38 beta or p38 gamma gene does not affect endurance exercise-induced fiber-type transformation. Wild type, p38 alpha, p38 beta, and p38 gamma MKO mice were subjected to 4 weeks of voluntary running (Ex) or sedentary cage activity (Sed) followed by fiber-type and immunoblot analyses in plantaris muscles. A) PCR of genomic DNA with the appropriate primers (see Materials and Methods) for the loxP flanked p38 alleles in wild type (WT), heterozygous (+/−) and homozygous (−/−) mice with loxP-flanked p38 alleles. Only the homozygous mice with the Cre transgene (not shown) were considered p38 muscle-specific knockout (MKO) mice; B) Immunoblot analysis for p38 alpha and p38 gamma protein in plantaris muscles of p38 MKO mice in comparison with the wild type littermates. alpha -tubulin was used as a loading control; and C) Images of immunofluorescence staining of plantaris muscle sections with antibodies against myosin heavy chain IIb (Green), IIa (Blue) and I (Red). Appreciable increases in the percentage of type IIa fibers with concurrent decreases in type IIb fibers are noted in Ex group compared with Sed group among all four genetic backgrounds. The quantitative data is presented in Table 1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19936205), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat p38 alpha Antibody

Application
Recommended Usage

Knockout Validated

p38 alpha is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in p38 alpha knockout HEK293T cell line.

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB869 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p38 alpha

p38 alpha (also known as MAPK14 or SAPK2A) is a member of the p38 MAPK family which are activated by various environmental stresses and pro-inflammatory cytokines (1). The activation of p38 requires its phosphorylation by MAP kinase kinases (MKKs) or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase (2). The substrates of p38 include transcription regulator ATF2, MEF2C, MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.

References

  1. Han, J. et al. (1994) Science 265:808.
  2. Ge, B. et al. (2002) Science 295:1291.

Alternate Names

MAPK14, SAPK2a

Entrez Gene IDs

1432 (Human); 26416 (Mouse); 81649 (Rat)

Gene Symbol

MAPK14

UniProt

Q16539

Additional p38 alpha Products

Product Documents for Human/Mouse/Rat p38 alpha Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat p38 alpha Antibody

For research use only

Citations for Human/Mouse/Rat p38 alpha Antibody

Customer Reviews for Human/Mouse/Rat p38 alpha Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Human/Mouse/Rat p38 alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: NIH‑3T3 mouse embryonic fibroblast cell line
    Species: Mouse
    Verified Customer | Posted 03/24/2022
    NIH‑3T3 mouse embryonic fibroblast cell line
    1 ug/mL dilution
    Human/Mouse/Rat p38 alpha Antibody MAB869
  • Human/Mouse/Rat p38 alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 11/29/2017

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