Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Knockout Validated, Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Dual RNAscope ISH-IHC Compatible, Immunocytochemistry, COMET

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 979517
View all Vimentin Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human Vimentin
Ser2-Glu466
Accession # P08670

Specificity

Detects human Vimentin in direct ELISAs. Detects human, mouse, and rat Vimentin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat Vimentin Antibody

Detection of Vimentin in Human Kidney via seqIF™ staining on COMET™​

Vimentin was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB21052) at 0.05 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder.

Detection of Vimentin in Human Liver via seqIF™ staining on COMET™​

Vimentin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB21052) at 0.05 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder.

Detection of Vimentin in Human Colon via seqIF™ staining on COMET™​

Vimentin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB21052) at 0.05 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder.
Detection of Human, Mouse, and Rat Vimentin antibody by Western Blot.

Detection of Human, Mouse, and Rat Vimentin by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB21052) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Vimentin antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Vimentin in HeLa Human Cell Line.

Vimentin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB21052) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Vimentin antibody in Human Lymph Node by Immunohistochemistry (IHC-P).

Vimentin in Human Lymph Node.

Vimentin was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB21052) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Vimentin Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line.

Vimentin Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

Vimentin was detected in immersion fixed K562 human chronic myelogenous leukemia cell line but is not detected in Vimentin knockout (KO) K562 Human Cell Line cell line using Mouse Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB21052) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Vimentin antibody in Human Tonsil Using Dual RNAscope®ISH and IHC.

Vimentin in Human Tonsil Using Dual RNAscope®ISH and IHC.

Vimentin mRNA (red) and protein (green) was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 310441) followed by immunohistochemistry using R&D Systems Mouse Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog# MAB21052) at 5ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001). Tissue was stained using ACD RNAscope®2.5 HD Duplex Detection Reagents (Catalog # 322500).

Applications for Human/Mouse/Rat Vimentin Antibody

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Dual RNAscope ISH-IHC Compatible

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node

Knockout Validated

Vimentin is specifically detected in K562 human chronic myelogenous leukemia cell line but is not detectable in Vimentin knockout K562 cell line.

Multiplex Immunofluorescence

0.05 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney, colon and liver

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line

Reviewed Applications

Read 4 reviews rated 4.8 using MAB21052 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Vimentin

Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine, and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9-11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).

References

  1. Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.
  2. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
  3. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.
  4. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
  5. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
  6. Li, Q-F. et al. (2006) J. Biol. Chem. 281:34716.
  7. Esue, O. et al. (2006) J. Biol. Chem. 281:30393.
  8. Styers, M.L. et al. (2005) Traffic 6:359.
  9. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
  10. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
  11. Ivaska, J. et al. (2005) EMBO J. 24:3834.
  12. Byun, Y. et al. (2001) Cell Death Differ. 8:443.
  13. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
  14. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
  15. Garg, A. et al. (2006) J. Immunol. 177:6192.

Alternate Names

VIM

Entrez Gene IDs

7431 (Human); 22352 (Mouse); 81818 (Rat)

Gene Symbol

VIM

UniProt

P08670

Additional Vimentin Products

Product Documents for Human/Mouse/Rat Vimentin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Vimentin Antibody

For research use only

Citations for Human/Mouse/Rat Vimentin Antibody

Customer Reviews for Human/Mouse/Rat Vimentin Antibody (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Human/Mouse/Rat Vimentin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Ovarian cancer tissue
    Species: Mouse
    Verified Customer | Posted 11/09/2021
    Human/Mouse/Rat Vimentin Antibody MAB21052
  • Human/Mouse/Rat Vimentin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Cancer Tissue
    Species: Mouse
    Verified Customer | Posted 06/18/2021
    Human/Mouse/Rat Vimentin Antibody MAB21052
  • Human/Mouse/Rat Vimentin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Pancreatic cancer cells
    Species: Human
    Verified Customer | Posted 02/20/2021
    Human/Mouse/Rat Vimentin Antibody MAB21052
  • Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Pancreatic cancer cells
    Species: Mouse
    Verified Customer | Posted 07/16/2018

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