Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 268014
View all NF-L Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NF-L
Met1-Asp338
Accession # P07196

Specificity

Detects human NF-L in direct ELISAs and human, mouse and rat NF-L in Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) NF‑H or rhNF‑M is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat NF‑L Antibody

Detection of Mouse and Rat NF‑L by Western Blot.

Western blot shows lysates of mouse brain (hypothalamus) and rat brain (hippocampus). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat NF‑L Monoclonal Antibody (Catalog # MAB2216) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NF‑L at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
NF‑L antibody in Human Spinal Cord by Immunohistochemistry (IHC-P).

NF‑L in Human Spinal Cord.

NF-L was detected in immersion fixed paraffin-embedded sections of human spinal cord using 25 µg/mL Mouse Anti-Human NF-L Monoclonal Antibody (Catalog # MAB2216) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counter-stained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in motor neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
NF-L antibody in Human Brain by Immunohistochemistry (IHC-P).

NF‑L in Human Brain.

NF-L was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human NF-L Monoclonal Antibody (Catalog # MAB2216) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

NF‑L in U251 human myeloma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative).

NF‑L was detected in immersion fixed U251 human myeloma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative) using Mouse Anti-Human/Mouse/Rat NF‑L Monoclonal Antibody (Catalog # MAB2216) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human/Mouse/Rat NF‑L Antibody

Application
Recommended Usage

Immunocytochemistry

8 µg/mL
Sample: Immersion fixed U251 human myeloma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative)

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spinal cord and immersion fixed paraffin-embedded sections of human brain (substantia nigra)

Western Blot

1 µg/mL
Sample: Mouse brain (hypothalamus) and rat brain (hippocampus)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NF-L

NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Entrez Gene IDs

4747 (Human)

Gene Symbol

NEFL

UniProt

P07196

Additional NF-L Products

Product Documents for Human/Mouse/Rat NF‑L Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat NF‑L Antibody

For research use only

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Protocols

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