Human Pro MMP-13 Antibody

Catalog # Availability Size / Price Qty
MAB913
MAB913-SP
Human Pro MMP-13 Antibody in ELISA
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Product Details
Citations (1)
FAQs
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Reviews (1)

Human Pro MMP-13 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pro MMP‑13 in direct ELISAs and Western blots. Does not detect active recombinant human MMP-13. In Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.
Source
Monoclonal Mouse IgG2B Clone # 87518
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-13
Leu20-Cys471
Accession # P45452
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP-13 Western Blot Standard (Catalog # WBC020) under non‑reducing conditions only
ELISA

This antibody functions as a Pro-MMP-13 ELISA ELISA detection antibody when paired with Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB5111).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pro-MMP-13 DuoSet ELISA Kit (Catalog # DY913) for convenient development of a sandwich ELISA or the Human Pro-MMP-13 Quantikine ELISA Kit (Catalog # DM1300) for a complete optimized ELISA.

 
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑13 (Catalog # 511‑MM), see our available Western blot detection antibodies
Immunoaffinity Purification
Sepharose beads coupled with this antibody have been used to immunoprecipitate and immunopurify Recombinant Human Pro-MMP-13 (Catalog # 511-MM) from condition media.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

HumanPro- MMP‑13 ELISA Standard Curve. Recombinant Human Pro-MMP‑13 protein was serially diluted 2-fold and captured by Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB5111) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pro MMP‑13 Monoclonal Antibody (Catalog # MAB913) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-13

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

References
  1. Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.
Long Name
Matrix Metalloproteinase 13
Entrez Gene IDs
4322 (Human)
Alternate Names
CLG 3; CLG3EC 3.4.24; collagenase 3; EC 3.4.24.-; EC 3.4.24.22; EC 3.4.24.24; EC 3.4.24.35; EC 3.4.24.65; EC 3.4.24.7; MANDP1; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP13; MMP-13

Product Datasheets

Citation for Human Pro MMP-13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Detection of changes in articular cartilage proteoglycan by T(1rho) magnetic resonance imaging.
    Authors: Wheaton AJ, Dodge GR, Borthakur A, Kneeland JB, Schumacher HR, Reddy R
    J. Orthop. Res., 2005;23(1):102-8.
    Species: Porcine
    Sample Types: Synovial Fluid
    Applications: Immunoprecipitation

FAQs

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Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Pro MMP-13 Antibody

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Human Pro MMP-13 Antibody
By Anonymous on 08/28/2018
Application: ICC/IF Sample Tested: C20A4 Chondrocyte cell line Species: Human