Human Pro MMP-13 Antibody Summary
Accession # P45452
This antibody functions as a Pro-MMP-13 ELISA ELISA detection antibody when paired with Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB5111).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pro-MMP-13 DuoSet ELISA Kit (Catalog # DY913) for convenient development of a sandwich ELISA or the Human Pro-MMP-13 Quantikine ELISA Kit (Catalog # DM1300) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human Pro- MMP‑13 ELISA Standard Curve. Recombinant Human Pro-MMP-13 protein was serially diluted 2-fold and captured by Mouse Anti-Human MMP-13 Monoclonal Antibody (Catalog # MAB5111) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pro MMP-13 Monoclonal Antibody (Catalog # MAB913) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.
- Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.
Citation for Human Pro MMP-13 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Detection of changes in articular cartilage proteoglycan by T(1rho) magnetic resonance imaging.
Authors: Wheaton AJ, Dodge GR, Borthakur A, Kneeland JB, Schumacher HR, Reddy R
J. Orthop. Res., 2005;23(1):102-8.
Sample Types: Synovial Fluid
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