Human TRAIL R4/TNFRSF10D Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TRAIL R4/TNFRSF10D in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, and rhTRAIL R3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TRAIL R4/TNFRSF10D
    Ala56-His211
    Accession # Q9UBN6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR)
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood granulocytes
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human brain (occipital cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize TRAIL R4/TNFRSF10D-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.03-0.1 µg/mL in the presence of 10 ng/mL Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
TRAIL R4/TNFRSF10D Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Human TRAIL R4/TNFRSF10D Antibody.

In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (5 µg/mL, Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375‑TL) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (12 ng/mL) activity elicited by Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL R4/
TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633). The ND50 is typically 0.03‑0.1 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL R4/TNFRSF10D

Human TRAIL R4, also called decoy receptor 2 (DcR2) and TRUNND (TRAIL receptor with a truncated death domain), is a type 1, TNF R family membrane protein, which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R4 is referred to as TNFRSF10D. TRAIL R4 is unique among the TRAIL receptors in that its cytoplasmic domain contains a truncated consensus death domain motif. Binding of TRAIL R4 does not result in an apoptotic signal. Overexpression of TRAIL R4 can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. The human soluble TRAIL R4/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

  • References:
    1. Griffith, T.S. et al. (1998) Curr. Opin. Immunol. 10:559.
    2. Pan, G. et al. (1998) FEBS lett 424:41.
    3. Marsters, S.A. et al. (1997) Cur. Biol. 7:1003.
    4. Degli-Esposti, M.A. et al. (1997) Immunity 7:813.
  • Long Name:
    TRAIL Receptor 4
  • Entrez Gene IDs:
    8793 (Human)
  • Alternate Names:
    CD264 antigen; CD264; DcR2; DCR2decoy with truncated death domain; Decoy receptor 2; TNF receptor-related receptor for TRAIL; TNF-related apoptosis-inducing ligand receptor 4; TNFRSF10D; TRAIL R4; TRAILR4; TRAIL-R4; TRAILR4TRAIL receptor 4; TRUNDDTRAIL receptor with a truncated death domain; tumor necrosis factor receptor superfamily member 10D; tumor necrosis factor receptor superfamily, member 10d, decoy with truncateddeath domain
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells.
    Authors: Secchiero P, Melloni E, Corallini F, Beltrami AP, Alviano F, Milani D, D'Aurizio F, di Iasio MG, Cesselli D, Bagnara GP, Zauli G
    Stem Cells, 2008;26(11):2955-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Differential response of p53 and p21 on HDAC inhibitor-mediated apoptosis in HCT116 colon cancer cells in vitro and in vivo.
    Authors: Zopf S, Neureiter D, Bouralexis S, Abt T, Glaser KB, Okamoto K, Ganslmayer M, Hahn EG, Herold C, Ocker M
    Int. J. Oncol., 2007;31(6):1391-402.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Acquired resistance to TRAIL-induced apoptosis in human ovarian cancer cells is conferred by increased turnover of mature caspase-3.
    Authors: Lane D, Cote M, Grondin R, Couture MC, Piche A
    Mol. Cancer Ther., 2006;5(3):509-21.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  4. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces rheumatoid arthritis synovial fibroblast proliferation through mitogen-activated protein kinases and phosphatidylinositol 3-kinase/Akt.
    Authors: Morel J, Audo R, Hahne M, Combe B
    J. Biol. Chem., 2005;280(16):15709-18.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Death receptors, Fas and TRAIL receptors, are involved in human osteoclast apoptosis.
    Authors: Roux S, Lambert-Comeau P, Saint-Pierre C, Lepine M, Sawan B, Parent JL
    Biochem. Biophys. Res. Commun., 2005;333(1):42-50.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) sequentially upregulates nitric oxide and prostanoid production in primary human endothelial cells.
    Authors: Zauli G, Pandolfi A, Gonelli A, Di Pietro R, Guarnieri S, Ciabattoni G, Rana R, Vitale M, Secchiero P
    Circ. Res., 2003;92(7):732-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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