Detects human TRAIL R4/TNFRSF10D in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, and rhTRAIL R3 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human TRAIL R4/TNFRSF10D Ala56-His211 Accession # Q9UBN6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR)
2.5 µg/106 cells
Human whole blood granulocytes
Immersion fixed paraffin-embedded sections of human brain (occipital cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Measured by its ability to neutralize TRAIL R4/TNFRSF10D-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.03-0.1 µg/mL in the presence of 10 ng/mL Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
TRAIL R4/TNFRSF10D Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Human TRAIL R4/TNFRSF10D Antibody.
In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (5 µg/mL, Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375‑TL) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (12 ng/mL) activity elicited by Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL R4/ TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633). The ND50 is typically 0.03‑0.1 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL R4/TNFRSF10D
Human TRAIL R4, also called decoy receptor 2 (DcR2) and TRUNND (TRAIL receptor with a truncated death domain), is a type 1, TNF R family membrane protein, which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R4 is referred to as TNFRSF10D. TRAIL R4 is unique among the TRAIL receptors in that its cytoplasmic domain contains a truncated consensus death domain motif. Binding of TRAIL R4 does not result in an apoptotic signal. Overexpression of TRAIL R4 can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. The human soluble TRAIL R4/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.
Griffith, T.S. et al. (1998) Curr. Opin. Immunol. 10:559.
Pan, G. et al. (1998) FEBS lett 424:41.
Marsters, S.A. et al. (1997) Cur. Biol. 7:1003.
Degli-Esposti, M.A. et al. (1997) Immunity 7:813.
TRAIL Receptor 4
Entrez Gene IDs:
CD264 antigen; CD264; DcR2; DCR2decoy with truncated death domain; Decoy receptor 2; TNF receptor-related receptor for TRAIL; TNF-related apoptosis-inducing ligand receptor 4; TNFRSF10D; TRAILR4; TRAIL-R4; TRAILR4TRAIL receptor 4; TRUNDDTRAIL receptor with a truncated death domain; tumor necrosis factor receptor superfamily member 10D; tumor necrosis factor receptor superfamily, member 10d, decoy with truncateddeath domain
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.