Porcine IL-6 Antibody

(8 citations)
(1 Review)
  
  • Species Reactivity
    Porcine
  • Specificity
    Detects porcine IL-6 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human IL‑6, recombinant mouse IL‑6, and recombinant rat IL‑6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant porcine IL-6
    Pro29-Met212
    Accession # P26893
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Porcine IL‑6 (Catalog # 686-PI)
    • Porcine IL-6 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Porcine IL‑6 Antibody (Catalog # AF686)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Porcine IL‑6 Biotinylated Antibody (Catalog # BAF686)
  • ELISA Standard
     
    Recombinant Porcine IL-6 Protein (Catalog # 686-PI)
  • Neutralization
    Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line [Nordan, R.P. and M. Potter (1986) Science 233:566]. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 12.5 ng/mL Recombinant Porcine IL‑6.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑6 and Neutralization by Porcine IL‑6 Antibody. Recombinant Porcine IL‑6 (Catalog # 686-PI) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑6 (12.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686). The ND50 is typically 0.15-0.6 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1 - 4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

  • References:
    1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
    2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
    3. Jones, S.A. (2005) J. Immunol. 175:3468.
    4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
    5. Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.
    6. Murakami, M. et al. (1993) Science 260:1808.
    7. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
    8. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  • Long Name:
    Interleukin 6
  • Entrez Gene IDs:
    3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
  • Alternate Names:
    B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Applications
Sample Type
  1. TRAF6 is a novel NS3-interacting protein that inhibits classical swine fever virus replication
    Authors: H Lv, W Dong, Z Cao, X Li, J Wang, G Qian, Q Lv, C Wang, K Guo, Y Zhang
    Sci Rep, 2017;7(1):6737.
    Species: Porcine
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  2. Differential Effects of Escherichia coli Nissle and Lactobacillus rhamnosus Strain GG on Human Rotavirus Binding, Infection, and B Cell Immunity.
    Authors: Kandasamy S, Vlasova A, Fischer D, Kumar A, Chattha K, Rauf A, Shao L, Langel S, Rajashekara G, Saif L
    J Immunol, 2016;196(4):1780-9.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Neut
  3. Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts.
    Authors: Yang YJ, Qian HY, Huang J, Geng YJ, Gao RL, Dou KF, Yang GS, Li JJ, Shen R, He ZX, Lu MJ, Zhao SH
    Eur. Heart J., 2008;29(12):1578-90.
    Species: Porcine
    Sample Type: Tissue Homogenates
    Application: WB
  4. In vitro induction of mucosa-type dendritic cells by all-trans retinoic acid.
    Authors: Saurer L, McCullough KC, Summerfield A
    J. Immunol., 2007;179(6):3504-14.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Neut
  5. The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
    Authors: Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E
    Intensive Care Med, 2007;33(6):1085-93.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  6. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
    Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
    J. Virol., 2006;80(1):372-82.
    Species: Porcine
    Sample Type: Serum
    Application: ELISA Development
  7. Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model.
    Authors: Carter AJ, Aggarwal M, Kopia GA, Tio F, Tsao PS, Kolata R, Yeung AC, Llanos G, Dooley J, Falotico R
    Cardiovasc. Res., 2004;63(4):617-24.
    Species: Porcine
    Sample Type: Tissue Homogenates
    Application: WB
  8. Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model.
    Authors: Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E
    Anesthesiology, 2004;100(4):861-70.
    Species: Porcine
    Sample Type: Plasma
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Porcine.
We have 1 review tested in 1 application: ELISA.

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ELISA Porcine IL-6 Antibody AF686
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 ELISA Porcine Anonymous 11/08/2017
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ELISA Porcine IL-6 Antibody AF686
ELISA: Porcine IL-6 Antibody [AF686]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesPorcine

Other Experimental Details

Other Experimental DetailsThe polyclonal antibody, AF696, was used as both the capture and detection antibody in a sandwich ELISA. It was specific and sensitive.

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