Key Product Details

Species Reactivity

Validated:

Porcine

Cited:

Human, Porcine

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Western Blot, Neutralization, ELISA Capture, ELISA Development, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant porcine IL-6
Pro29-Met212
Accession # P26893

Specificity

Detects porcine IL-6 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human IL‑6, recombinant mouse IL‑6, and recombinant rat IL‑6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Porcine IL‑6 Antibody

Cell Proliferation Induced by IL‑6 and Neutralization by Porcine IL‑6 Antibody.

Cell Proliferation Induced by IL‑6 and Neutralization by Porcine IL‑6 Antibody.

Recombinant Porcine IL‑6 (Catalog # 686-PI) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑6 (12.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686). The ND50 is typically 0.15-0.6 µg/mL.

Applications for Porcine IL‑6 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Porcine IL‑6 (Catalog # 686-PI)

Neutralization

Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line [Nordan, R.P. and M. Potter (1986) Science 233:566]. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 12.5 ng/mL Recombinant Porcine IL‑6.

Porcine IL-6 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Porcine IL‑6 Biotinylated Antibody (Catalog # BAF686)
  • Standard: Recombinant Porcine IL-6 Protein (Catalog # 686-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using AF686 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1 - 4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

References

  1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
  2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
  3. Jones, S.A. (2005) J. Immunol. 175:3468.
  4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
  5. Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.
  6. Murakami, M. et al. (1993) Science 260:1808.
  7. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  8. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

UniProt

P26893

Additional IL-6 Products

Product Documents for Porcine IL‑6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Porcine IL‑6 Antibody

For research use only

Citations for Porcine IL‑6 Antibody

Customer Reviews for Porcine IL‑6 Antibody (2)

5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Porcine IL-6 Antibody
    Name: Zoia Levashova
    Application: MSD assay
    Sample Tested: Vitreous humor
    Species: Porcine
    Verified Customer | Posted 10/18/2018
    After biotinylation, used as a capture reagent according to the manufacturer’s protocol &amp;;Meso Scale Diagnostics LLC;. A standard curve with porcine IL-6 from RnD ;Cat# 686-PI/CF; is shown;2-25,000 pg/ml;.
    Porcine IL‑6 Antibody AF686
  • Porcine IL-6 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Porcine
    Verified Customer | Posted 11/08/2017
    The polyclonal antibody, AF696, was used as both the capture and detection antibody in a sandwich ELISA. It was specific and sensitive.
    Porcine IL‑6 Antibody AF686

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