Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1 - 4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).
Key Product Details
Species Reactivity
Validated:
Porcine
Cited:
Human, Porcine
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Western Blot, Neutralization, ELISA Capture, ELISA Development, ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant porcine IL-6
Pro29-Met212
Accession # P26893
Pro29-Met212
Accession # P26893
Specificity
Detects porcine IL-6 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human IL‑6, recombinant mouse IL‑6, and recombinant rat IL‑6 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Porcine IL-6 Antibody
Cell Proliferation Induced by IL‑6 and Neutralization by Porcine IL‑6 Antibody.
Recombinant Porcine IL‑6 (Catalog # 686-PI) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑6 (12.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686). The ND50 is typically 0.15-0.6 µg/mL.
Porcine IL-6 ELISA Standard Curve
Recombinant Porcine IL‑6 (Catalog # 686-PI) was serially diluted and captured by Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Porcine IL-6 ELISA Standard Curve
Recombinant Porcine IL‑6 (Catalog # 686-PI) was serially diluted and captured by Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Porcine IL-6 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Porcine IL‑6 (Catalog # 686-PI)
Sample: Recombinant Porcine IL‑6 (Catalog # 686-PI)
Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line [Nordan, R.P. and M. Potter (1986) Science 233:566]. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 12.5 ng/mL Recombinant Porcine IL‑6.
Porcine IL-6 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using AF686 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-6
References
- Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
- Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
- Jones, S.A. (2005) J. Immunol. 175:3468.
- Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
- Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.
- Murakami, M. et al. (1993) Science 260:1808.
- Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
- Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
Long Name
Interleukin 6
Alternate Names
BSF-2, BSF2, IFNB2, IL6, MGI-2A
Entrez Gene IDs
Gene Symbol
IL6
UniProt
Additional IL-6 Products
Product Documents for Porcine IL-6 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Porcine IL-6 Antibody
For research use only
Related Research Areas
Citations for Porcine IL-6 Antibody
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Customer Reviews for Porcine IL-6 Antibody (2)
5 out of 5
2 Customer Ratings
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Application: MSD assaySample Tested: Vitreous humorSpecies: PorcineVerified Customer | Posted 10/18/2018After biotinylation, used as a capture reagent according to the manufacturer’s protocol &;Meso Scale Diagnostics LLC;. A standard curve with porcine IL-6 from RnD ;Cat# 686-PI/CF; is shown;2-25,000 pg/ml;.
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Application: ELISASample Tested: Serum and PlasmaSpecies: PorcineVerified Customer | Posted 11/08/2017The polyclonal antibody, AF696, was used as both the capture and detection antibody in a sandwich ELISA. It was specific and sensitive.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Jak/STAT Signaling Pathway
Mesenchymal Stem Cell Differentiation Pathways & Lineage-specific Markers
NOD-like Receptor Signaling Pathways
Th17 Differentiation Pathway
Toll-Like Receptor Signaling Pathways
