Bovine IL-4 Antibody Summary
Accession # P30367.2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Bovine IL‑4 by Western Blot. Western blot shows Recombinant Bovine IL-4 (Catalog # 2469-BL) (10 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Bovine IL-4 Monoclonal Antibody (Catalog # MAB2469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-4 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IL‑4 in Bovine PBMCs. IL-4 was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) stimulated with concanavalin A using Mouse Anti-Bovine IL-4 Monoclonal Antibody (Catalog # MAB2469) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Bovine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Alternate splicing generates two additional isoforms with internal deletions (5). Mature bovine IL-4 shares 60%, 91%, 93%, 78%, 55%, 39%, and 41% aa sequence identity with equine, goat, ovine, porcine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8‑11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).
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