Human Cardiotrophin-1/CT-1 Antibody Summary
Accession # Q16619
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This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Cardiotrophin-1/CT-1 Monoclonal Antibody (Catalog # MAB2603).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Cardiotrophin‑1/CT‑1 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cardiotrophin-1/CT-1 Monoclonal Antibody (Catalog # MAB2602) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cardiotrophin-1/CT-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cardiotrophin‑1/CT‑1 in Human Heart. Cardiotrophin-1/CT-1 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Cardiotrophin-1/CT-1 Monoclonal Antibody (Catalog # MAB2602) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Human Cardiotrophin-1/CT-1 ELISA Standard Curve. Recombinant human Cardiotrophin/CT-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Cardiotrophin/CT-1 Monoclonal Antibody (Catalog # MAB2603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Cardiotrophin/CT-1 Monoclonal Antibody (Catalog # MAB2602) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has been implicated in the receptor complex.
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