Human Cardiotrophin-1/CT-1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Cardiotrophin‑1/CT‑1 by Western Blot.
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Human Cardiotrophin-1/CT-1 Antibody Summary

Species Reactivity
Detects human Cardiotrophin-1 in direct ELISAs and Western blots.
Monoclonal Mouse IgG2A Clone # 89221
Protein A or G purified from ascites
E. coli-derived recombinant human Cardiotrophin-1
Accession # Q16619
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
2 µg/mL
See below

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Cardiotrophin-1/CT-1 Monoclonal Antibody (Catalog # MAB2603).

This product is intended for assay development on various assay platforms requiring antibody pairs.

5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cardiotrophin-1/CT-1 antibody by Western Blot. View Larger

Detection of Human Cardiotrophin‑1/CT‑1 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cardiotrophin-1/CT-1 Monoclonal Antibody (Catalog # MAB2602) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cardiotrophin-1/CT-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cardiotrophin-1/CT-1 antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

Cardiotrophin‑1/CT‑1 in Human Heart. Cardiotrophin-1/CT-1 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Cardiotrophin-1/CT-1 Monoclonal Antibody (Catalog # MAB2602) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human Cardiotrophin-1/CT-1 ELISA Standard Curve. Recombinant human Cardiotrophin/CT-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Cardiotrophin/CT-1 Monoclonal Antibody (Catalog # MAB2603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Cardiotrophin/CT-1 Monoclonal Antibody (Catalog # MAB2602) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cardiotrophin-1/CT-1

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third  alpha receptor subunit has been implicated in the receptor complex.

  1. Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
  2. Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.
Entrez Gene IDs
1489 (Human); 13019 (Mouse)
Alternate Names
cardiophin 1; cardiotrophin 1; Cardiotrophin1; Cardiotrophin-1; CT-1CT1; CTF1

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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Human Cardiotrophin-1/CT-1 Antibody
By Anonymous on 11/10/2021
Application: IHC Sample Tested: Human carotid artery Species: Human