Human CCL2/JE/MCP-1 Antibody
Human CCL2/JE/MCP-1 Antibody Summary
Applications
Human CCL2/JE/MCP-1 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
CCL2/JE/MCP‑1 in Human Crohn's Disease Intestine. CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB679) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
View Larger
Chemotaxis Induced by CCL2/MCP-1 and Neutralization by Human CCL2/MCP‑1 Antibody. Recombinant Human CCL2/MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679). The ND50 is typically 0.5-2.0 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1
CCL2 is a beta -chemokine that is also known as monocyte chemotactic protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes.
Product Datasheets
Citations for Human CCL2/JE/MCP-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
28
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Integrated DNA and RNA sequencing reveals early drivers involved in metastasis of gastric cancer
Authors: J Zhang, F Liu, Y Yang, N Yu, X Weng, Y Yang, Z Gong, S Huang, L Gan, S Sun, X Zhang, Y Gong, Y Liu, W Guo
Cell Death & Disease, 2022;13(4):392.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization -
Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case
Authors: S Abouelasra, M De Bondt, N Berghmans, M Gouwy, VLS de Oliveir, SC Oliveira, FA Amaral, P Proost, J Van Damme, S Struyf, M De Buck
Mediators Inflamm., 2020;2020(0):6087109.
Species: Human
Sample Types: Cell Culture Su
Applications: ELISA -
Soft extracellular matrix enhances inflammatory activation of mesenchymal stromal cells to induce monocyte production and trafficking
Authors: SW Wong, S Lenzini, MH Cooper, DJ Mooney, JW Shin
Sci Adv, 2020;6(15):eaaw0158.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases
Authors: B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg
Psychoneuroendocrinology, 2018;9(1):2705.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1? and MCP-1 in cultured human retinal pigment epithelial cells
Authors: ZM Bian, MG Field, SG Elner, JM Kahlenberg, VM Elner
Exp. Eye Res., 2018;170(0):29-39.
Species: Human
Sample Types: Cell Lysate
Applications: Western Blot -
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??.
Authors: Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang
Molecular Medicine Reports, 2018;0(0):1791-3004.
Species: Human
Sample Types: Cell Lysate
Applications: Western Blot -
A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells
Authors: HK Kim, SG Lee, SW Lee, BJ Oh, JH Kim, JA Kim, G Lee, JD Jang, YA Joe
Stem Cells, 2018;0(0):.
-
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??
Authors: B Liu, L Xu, X Yu, W Li, X Sun, S Xiao, M Guo, H Wang
Mol Med Rep, 2018;18(2):1987-1994.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
CCL2 influences the sensitivity of lung cancer A549 cells to docetaxel
Authors: T Wang, Q Zhan, X Peng, Z Qiu, T Zhao
Oncol Lett, 2018;16(1):1267-1274.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
Authors: CM Henry, SJ Martin
Mol. Cell, 2017;65(4):715-729.e5.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis
Authors: Y van Sleen, Q Wang, KSM van der Ge, J Westra, WH Abdulahad, P Heeringa, AMH Boots, E Brouwer
Sci Rep, 2017;7(1):6553.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
CCL2 as a potential therapeutic target for clear cell renal cell carcinoma
Cancer Med, 2016;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Evidence for chemokine synergy during neutrophil migration in ARDS
Thorax, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
Sci Rep, 2016;6(0):29909.
Species: Human
Sample Types: Serum
-
Microparticles engineered to highly express peroxisome proliferator-activated receptor-gamma decreased inflammatory mediator production and increased adhesion of recipient monocytes.
Authors: Sahler J, Woeller C, Phipps R
PLoS ONE, 2014;9(11):e113189.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010;56(0):1320.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development -
Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects.
Authors: Struyf S, Proost P, Vandercappellen J
Eur. J. Immunol., 2009;39(3):843-57.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
J. Immunol., 2009;183(9):5799-806.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Ultrasensitive flow-based immunoassays using single-molecule counting.
Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clin. Chem., 2007;53(11):1990-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007;329(1):214-8.
Species: Human
Sample Types: Serum
Applications: Luminex Development -
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clin. Chem., 2007;53(11):2010-2.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
Biochem. Biophys. Res. Commun., 2007;358(2):528-33.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
Blood, 2006;108(3):928-35.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development -
HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS.
Authors: Eugenin EA, Dyer G, Calderon TM, Berman JW
Glia, 2005;49(4):501-10.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model.
Authors: Carter AJ, Aggarwal M, Kopia GA, Tio F, Tsao PS, Kolata R, Yeung AC, Llanos G, Dooley J, Falotico R
Cardiovasc. Res., 2004;63(4):617-24.
Species: Porcine
Sample Types: Tissue Homogenates
Applications: Western Blot -
Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
Blood, 2004;104(2):314-20.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
trans fatty acids and systemic inflammation in heart failure.
Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
Am. J. Clin. Nutr., 2004;80(6):1521-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human CCL2/JE/MCP-1 Antibody
Average Rating: 5 (Based on 3 Reviews)
Have you used Human CCL2/JE/MCP-1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC).
Paired with SulfoTag-modified AF-279-NA as a detection antibody. Calibration curves with Recombinant Human CCL2 (279-MC-010/CF) is shown (dynamic range 6-25,000 pg/ml)
