Human CCL2/JE/MCP-1 Antibody

Catalog # Availability Size / Price Qty
MAB679-SP
MAB679-100
MAB679-500
CCL2/JE/MCP‑1 in Human Crohn's Disease Intestine.
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Product Details
Citations (27)
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Reviews (3)

Human CCL2/JE/MCP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL2/JE/MCP-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) CCL2, 3, 4, rhCCL3, 4, 5, 7, or 8.
Source
Monoclonal Mouse IgG2B Clone # 23007
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL2/JE/MCP-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC)
Immunohistochemistry
5-15 µg/mL
See below

Human CCL2/JE/MCP-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL2/JE/MCP‑1 Biotinylated Antibody (Catalog # BAF279)

Standard: Recombinant Human CCL2/MCP-1 Protein (Catalog # 279-MC)

Neutralization
Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 75 ng/mL Recombinant Human CCL2/JE/MCP‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry CCL2/JE/MCP-1 antibody in Human Crohn's Disease Intestine by Immunohistochemistry (IHC-P). View Larger

CCL2/JE/MCP‑1 in Human Crohn's Disease Intestine. CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB679) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Chemotaxis Induced by CCL2/MCP-1 and Neutralization by Human CCL2/MCP‑1 Antibody. View Larger

Chemotaxis Induced by CCL2/MCP-1 and Neutralization by Human CCL2/MCP‑1 Antibody. Recombinant Human CCL2/MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679). The ND50 is typically 0.5-2.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

CCL2 is a beta -chemokine that is also known as monocyte chemotactic protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes.

Entrez Gene IDs
6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

Product Datasheets

Citations for Human CCL2/JE/MCP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case
    Authors: S Abouelasra, M De Bondt, N Berghmans, M Gouwy, VLS de Oliveir, SC Oliveira, FA Amaral, P Proost, J Van Damme, S Struyf, M De Buck
    Mediators Inflamm., 2020;2020(0):6087109.
    Species: Human
    Sample Types: Cell Culture Su
    Applications: ELISA
  2. Soft extracellular matrix enhances inflammatory activation of mesenchymal stromal cells to induce monocyte production and trafficking
    Authors: SW Wong, S Lenzini, MH Cooper, DJ Mooney, JW Shin
    Sci Adv, 2020;6(15):eaaw0158.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases
    Authors: B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg
    Psychoneuroendocrinology, 2018;9(1):2705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1? and MCP-1 in cultured human retinal pigment epithelial cells
    Authors: ZM Bian, MG Field, SG Elner, JM Kahlenberg, VM Elner
    Exp. Eye Res., 2018;170(0):29-39.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Western Blot
  5. Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??.
    Authors: Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang
    Molecular Medicine Reports, 2018;0(0):1791-3004.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Western Blot
  6. A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells
    Authors: HK Kim, SG Lee, SW Lee, BJ Oh, JH Kim, JA Kim, G Lee, JD Jang, YA Joe
    Stem Cells, 2018;0(0):.
  7. Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??
    Authors: B Liu, L Xu, X Yu, W Li, X Sun, S Xiao, M Guo, H Wang
    Mol Med Rep, 2018;18(2):1987-1994.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. CCL2 influences the sensitivity of lung cancer A549 cells to docetaxel
    Authors: T Wang, Q Zhan, X Peng, Z Qiu, T Zhao
    Oncol Lett, 2018;16(1):1267-1274.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  9. Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis
    Authors: Y van Sleen, Q Wang, KSM van der Ge, J Westra, WH Abdulahad, P Heeringa, AMH Boots, E Brouwer
    Sci Rep, 2017;7(1):6553.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
    Authors: CM Henry, SJ Martin
    Mol. Cell, 2017;65(4):715-729.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. CCL2 as a potential therapeutic target for clear cell renal cell carcinoma
    Cancer Med, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
    Sci Rep, 2016;6(0):29909.
    Species: Human
    Sample Types: Serum
  13. Evidence for chemokine synergy during neutrophil migration in ARDS
    Thorax, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Microparticles engineered to highly express peroxisome proliferator-activated receptor-gamma decreased inflammatory mediator production and increased adhesion of recipient monocytes.
    Authors: Sahler J, Woeller C, Phipps R
    PLoS ONE, 2014;9(11):e113189.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  15. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  16. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009;183(9):5799-806.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  17. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  18. Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects.
    Authors: Struyf S, Proost P, Vandercappellen J
    Eur. J. Immunol., 2009;39(3):843-57.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  19. Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
    Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
    Biochem. Biophys. Res. Commun., 2007;358(2):528-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  21. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  22. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  23. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
    Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
    Blood, 2006;108(3):928-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  24. HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS.
    Authors: Eugenin EA, Dyer G, Calderon TM, Berman JW
    Glia, 2005;49(4):501-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model.
    Authors: Carter AJ, Aggarwal M, Kopia GA, Tio F, Tsao PS, Kolata R, Yeung AC, Llanos G, Dooley J, Falotico R
    Cardiovasc. Res., 2004;63(4):617-24.
    Species: Porcine
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  26. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004;80(6):1521-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  27. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004;104(2):314-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human CCL2/JE/MCP-1 Antibody

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Human CCL2/JE/MCP-1 Antibody
By Anonymous on 08/17/2021
Application: WB Sample Tested: Hepatocellular carcinoma cells Species: Human

Human CCL2/JE/MCP-1 Antibody
By Anonymous on 11/20/2018
Application: MSD assay Sample Tested: Vitreous humor Species: Cynomolgus Monkey

After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC).
Paired with SulfoTag-modified AF-279-NA as a detection antibody. Calibration curves with Recombinant Human CCL2 (279-MC-010/CF) is shown (dynamic range 6-25,000 pg/ml)


Human CCL2/JE/MCP-1 Antibody
By Anonymous on 12/13/2017
Application: ELISA Sample Tested: CHO Chinese hamster ovary cell line Species: Hamster