Human CCL2/JE/MCP-1 Antibody

(18 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL2/JE/MCP-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) CCL2, 3, 4, rhCCL3, 4, 5, 7, or 8.
  • Source
    Monoclonal Mouse IgG2B Clone # 23007
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CCL2/JE/MCP-1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC)
    • Human CCL2/JE/MCP-1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human CCL2/JE/MCP‑1 Antibody (Catalog # MAB679)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human CCL2/JE/MCP‑1 Biotinylated Antibody (Catalog # BAF279)
  • ELISA Standard
     
    Recombinant Human CCL2/MCP-1 Protein (Catalog # 279-MC)
  • Neutralization
    Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 0.15 µg/mL Recombinant Human CCL2/JE/MCP‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL2/MCP-1 and Neutralization by Human CCL2/MCP‑1 Antibody. Recombinant Human CCL2/MCP‑1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP‑1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP‑1 Monoclonal Antibody (Catalog # MAB679). The ND50 is typically 0.5-2.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1
CCL2 is a beta -chemokine that is also known as monocyte chemotactic protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes.
  • Entrez Gene IDs:
    6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
  • Alternate Names:
    CCL2; MCAF; MCP-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis
    Authors: Y van Sleen, Q Wang, KSM van der Ge, J Westra, WH Abdulahad, P Heeringa, AMH Boots, E Brouwer
    Sci Rep, 2017;7(1):6553.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. CCL2 as a potential therapeutic target for clear cell renal cell carcinoma
    Cancer Med, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  3. Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
    Sci Rep, 2016;6(0):29909.
    Species: Human
    Sample Type: Serum
  4. Evidence for chemokine synergy during neutrophil migration in ARDS
    Thorax, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Microparticles engineered to highly express peroxisome proliferator-activated receptor-gamma decreased inflammatory mediator production and increased adhesion of recipient monocytes.
    Authors: Sahler J, Woeller C, Phipps R
    PLoS ONE, 2014;9(11):e113189.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  6. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  7. Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects.
    Authors: Struyf S, Proost P, Vandercappellen J
    Eur. J. Immunol., 2009;39(3):843-57.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  8. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Type: Urine
    Application: Antibody Array Development
  9. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009;183(9):5799-806.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  10. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  11. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Type: Serum
    Application: Luminex Development
  12. Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
    Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
    Biochem. Biophys. Res. Commun., 2007;358(2):528-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  14. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
    Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
    Blood, 2006;108(3):928-35.
    Species: Human
    Sample Type: Whole Cells
    Application: ELISA Development
  15. HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS.
    Authors: Eugenin EA, Dyer G, Calderon TM, Berman JW
    Glia, 2005;49(4):501-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  16. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004;104(2):314-20.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  17. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004;80(6):1521-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  18. Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model.
    Authors: Carter AJ, Aggarwal M, Kopia GA, Tio F, Tsao PS, Kolata R, Yeung AC, Llanos G, Dooley J, Falotico R
    Cardiovasc. Res., 2004;63(4):617-24.
    Species: Porcine
    Sample Type: Tissue Homogenates
    Application: WB
Expand to show all 18 Citations
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

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