Human ERK1 Antibody

Detection of Human ERK1 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human ERK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1879) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of ERK1 by Western Blot

Western blot analysis of p-CREB, CREB, p-EGFR, EGFR, p-ERK1/2, ERK1, ERK2, EP4, and MRP4 in OVCAR-5 cells. Cells were treated with the indicated agents for 24 h, then light-activated (0.1 J/cm2, 10 mW/cm2) or maintained in dark conditions. After 24 h, cells were agonized with EGF (50 ng/mL) and PGE2 (1 µM) for 10 min, then whole extracts were collected and analyzed using Western blot. (A) Representative Western blot images and (B–K) relative densitometric bar graphs of phosphorylated and total proteins were shown. Results are normalized to the vehicle control group. Statistical analysis was performed using a one-way ANOVA and post hoc Tukey’s test. Percentages below each band represent the average change in intensity relative to the vehicle control across all experiments. For pERK1 and pERK2 bands, the first number corresponds to pERK1, and the second number corresponds to pERK2. Error bars represent the standard error of the mean. * p ≤ 0.05; ns: nonsignificant. Original western blot images (Supplementary Figure S4). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34771424), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of ERK1 by Western Blot

Western blot analysis of p-CREB, CREB, p-EGFR, EGFR, p-ERK1/2, ERK1, ERK2, EP4, and MRP4 in OVCAR-5 cells. Cells were treated with the indicated agents for 24 h, then light-activated (0.1 J/cm2, 10 mW/cm2) or maintained in dark conditions. After 24 h, cells were agonized with EGF (50 ng/mL) and PGE2 (1 µM) for 10 min, then whole extracts were collected and analyzed using Western blot. (A) Representative Western blot images and (B–K) relative densitometric bar graphs of phosphorylated and total proteins were shown. Results are normalized to the vehicle control group. Statistical analysis was performed using a one-way ANOVA and post hoc Tukey’s test. Percentages below each band represent the average change in intensity relative to the vehicle control across all experiments. For pERK1 and pERK2 bands, the first number corresponds to pERK1, and the second number corresponds to pERK2. Error bars represent the standard error of the mean. * p ≤ 0.05; ** p ≤ 0.01; ns: nonsignificant. Original western blot images (Supplementary Figure S5). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34771424), licensed under a CC-BY license. Not internally tested by R&D Systems.