Human/Mouse/Rat Bcl-2 Antibody
Human/Mouse/Rat Bcl-2 Antibody Summary
Ala2-Asp211
Accession # P10415
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Bcl‑2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, KG-1 human acute myelogenous leukemia cell line, CTLL-2 mouse cytotoxic T cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Bcl-2 Monoclonal Antibody (Catalog # MAB8272) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bcl-2 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Bcl‑2 in Human Lymphoma. Bcl-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Mouse Anti-Human/Mouse/Rat Bcl-2 Monoclonal Antibody (Catalog # MAB8272) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Western Blot Shows Human Bcl‑2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bcl-2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Bcl-2 Monoclonal Antibody (Catalog # MAB8272) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Bcl-2 at approximately 24 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-2
Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
Product Datasheets
Citations for Human/Mouse/Rat Bcl-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Human Umbilical Cord Mesenchymal Stem Cells Attenuate Severe Burn-Induced Multiple Organ Injury via Potentiating IGF-1 and BCL-2/BAX Pathway
Authors: H Wang, T Ba, Q Wang, L Yang, C Li, X Hao, Y Yin, L Liu
Stem Cells International, 2022;2022(0):5474289.
Species: Rat
Sample Types: Tissue Lysates
Applications: Western Blot -
Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
International immunopharmacology, 2021;103(0):108494.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Curcumin alleviates arsenic-induced toxicity in PC12�cells via modulating autophagy/apoptosis
Authors: MS Rahaman, S Banik, M Akter, MM Rahman, MT Sikder, T Hosokawa, T Saito, M Kurasaki
Ecotoxicol. Environ. Saf., 2020;200(0):110756.
Species: Rat
Sample Types: Whole Cell
Applications: Cell Culture -
Metformin exhibited anticancer activity by lowering cellular cholesterol content in breast cancer cells
Authors: A Sharma, S Bandyopadh, K Chowdhury, T Sharma, R Maheshwari, A Das, G Chakrabart, V Kumar, CC Mandal
PLoS ONE, 2019;14(1):e0209435.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
FK506 Attenuates the MRP1-Mediated Chemoresistant Phenotype in Glioblastoma Stem-Like Cells
Authors: Á Torres, V Arriagada, JI Erices, MLÁ Toro, JD Rocha, I Niechi, C Carrasco, C Oyarzún, C Quezada
Int J Mol Sci, 2018;19(9):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Dexmedetomidine protects the developing rat brain against the neurotoxicity wrought by sevoflurane: role of autophagy and Drp1-Bax signaling
Authors: Y Shan, S Sun, F Yang, N Shang, H Liu
Drug Des Devel Ther, 2018;12(0):3617-3624.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Exosomes derived from human umbilical cord mesenchymal stem cells improve myocardial repair via upregulation of Smad7
Authors: XL Wang, YY Zhao, L Sun, Y Shi, ZQ Li, XD Zhao, CG Xu, HG Ji, M Wang, WR Xu, W Zhu
Int. J. Mol. Med., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Hepatoprotective effect of calculus bovis sativus on nonalcoholic fatty liver disease in mice by inhibiting oxidative stress and apoptosis of hepatocytes
Authors: W He, Y Xu, C Zhang, J Lu, J Li, D Xiang, J Yang, M Chang, D Liu
Drug Des Devel Ther, 2017;11(0):3449-3460.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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