Human TGF-beta  RII Antibody

(15 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) TGF‑ beta  RII and less than 1% cross-reactivity with recombinant human TGF-beta RIII and rmTGF-beta RI is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RII
    Ile24-Asp159
    Accession # P37173.2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.01 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    1 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Human TGF-beta RII Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human TGF‑ beta  RII Antibody (Catalog # AF-241-NA)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human TGF‑ beta  RII Biotinylated Antibody (Catalog # BAF241)
  • ELISA Standard
     
    Recombinant Human TGF-beta RII Protein (Catalog # 241-R2)
  • Neutralization
    Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the TF‑1 human erythroleukemic cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 5‑20 µg/mL in the presence of 0.04 ng/mL Recombinant Human TGF‑ beta 1 and 5 ng/mL Recombinant Human IL‑4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TGF‑ beta  RII by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TGF‑ beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF‑ beta  RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TGF-beta  RII in Human Lymphocytes by Flow Cytometry. Human blood-derived lymphocytes were labeled with Goat Anti-Human TGF-beta  RII Antigen Affinity-purified Poly­clonal Antibody (Catalog #
AF‑241‑NA, filled histogram) or control antibody (Catalog #
AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Immunohistochemistry
TGF-beta  RII in Human Pituitary. TGF-beta  RII was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human TGF-beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Human TGF‑ beta  RII Antibody. Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Human IL‑4 (Catalog # 204-IL) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑4 (5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.04 ng/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti- Human TGF‑ beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA). The ND50 is typically
10‑20 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RII

TGF beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF beta RII interacts with TGF beta RI to form the heteromeric signaling complex thattransduces TGF beta signals. A splice variant of the type II receptor, TGF beta RIIb, containing a 25 amino acid residue insertion near the Nterminus of the mature protein has also been described.

  • Long Name:
    Transforming Growth Factor beta Receptor II
  • Entrez Gene IDs:
    7048 (Human); 21813 (Mouse)
  • Alternate Names:
    AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. 24R,25-Dihydroxyvitamin D3 Protects against Articular Cartilage Damage following Anterior Cruciate Ligament Transection in Male Rats
    PLoS ONE, 2016;11(8):e0161782.
    Species: Rat
    Sample Type: Whole Cells
    Application: Blocking
  2. Clathrin light chains' role in selective endocytosis influences antibody isotype switching
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Integrin-mediated type II TGF-beta receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling.
    Authors: Chen X, Wang H, Liao H, Hu W, Gewin L, Mernaugh G, Zhang S, Zhang Z, Vega-Montoto L, Vanacore R, Fassler R, Zent R, Pozzi A
    J Clin Invest, 2014;124(8):3295-310.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Neut
  4. Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of alphavbeta6 integrin in DCIS identifies high-risk patients and predicts recurrence.
    Authors: Allen M, Thomas G, Clark S, Dawoud M, Vallath S, Payne S, Gomm J, Dreger S, Dickinson S, Edwards D, Pennington C, Sestak I, Cuzick J, Marshall J, Hart I, Jones J
    Clin Cancer Res, 2014;20(2):344-57.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  5. CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGFbeta / GARP-mediated mechanism.
    Authors: Miller M, Petty C, Tompkins M, Fogle J
    Virol J, 2014;11(0):7.
    Species: Feline
    Sample Type: Whole Cells
    Application: Neut
  6. T-cell TGF-beta signaling abrogation restricts medulloblastoma progression.
    Authors: Gate D, Danielpour M, Rodriguez J, Kim G, Levy R, Bannykh S, Breunig J, Kaech S, Flavell R, Town T
    Proc Natl Acad Sci U S A, 2014;111(33):E3458-66.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  7. Mesothelial cells promote early ovarian cancer metastasis through fibronectin secretion.
    Authors: Kenny H, Chiang C, White E, Schryver E, Habis M, Romero I, Ladanyi A, Penicka C, George J, Matlin K, Montag A, Wroblewski K, Yamada S, Mazar A, Bowtell D, Lengyel E
    J Clin Invest, 2014;124(10):4614-28.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells.
    Authors: Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F
    Cancer Res, 2013;73(7):2333-44.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis.
    Authors: Rai D, Kim SW, McKeller MR
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3111-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. Antibody-mediated blockade of integrin alpha v beta 6 inhibits tumor progression in vivo by a transforming growth factor-beta-regulated mechanism.
    Authors: Van Aarsen LA, Leone DR, Ho S, Dolinski BM, McCoon PE, LePage DJ, Kelly R, Heaney G, Rayhorn P, Reid C, Simon KJ, Horan GS, Tao N, Gardner HA, Skelly MM, Gown AM, Thomas GJ, Weinreb PH, Fawell SE, Violette SM
    Cancer Res., 2008;68(2):561-70.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Tumor-induced anorexia and weight loss are mediated by the TGF-beta superfamily cytokine MIC-1.
    Authors: Johnen H, Lin S, Kuffner T, Brown DA, Tsai VW, Bauskin AR, Wu L, Pankhurst G, Jiang L, Junankar S, Hunter M, Fairlie WD, Lee NJ, Enriquez RF, Baldock PA, Corey E, Apple FS, Murakami MM, Lin EJ, Wang C, During MJ, Sainsbury A, Herzog H, Breit SN
    Nat. Med., 2007;13(11):1333-40.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  12. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2005;105(2):750-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  13. Very low O2 concentration (0.1%) favors G0 return of dividing CD34+ cells.
    Authors: Hermitte F, Brunet de la Grange P, Belloc F, Praloran V, Ivanovic Z
    Stem Cells, 2005;24(1):65-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  14. TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing.
    Authors: Song QH, Klepeis VE, Trinkaus-Randall V
    MP, Mol. Pathol., 2002;55(3):164-76.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC
  15. Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways.
    Authors: Rosado E, Schwartz Z, Sylvia VL, Dean DD, Boyan BD
    Biochim. Biophys. Acta, 2002;1590(1):1-15.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 15 Citations
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