|Detection of Human TGF‑ beta RII by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TGF‑ beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF‑ beta RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Detection of TGF-beta RII in Human Lymphocytes by Flow Cytometry. Human blood-derived lymphocytes were labeled with Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog #|
AF‑241‑NA, filled histogram) or control antibody (Catalog #
AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
|TGF-beta RII in Human Pituitary. TGF-beta RII was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Human TGF‑ beta RII Antibody. Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Human IL‑4 (Catalog # 204-IL) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑4 (5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.04 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti- Human TGF‑ beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA). The ND50 is typically|
TGF beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF beta RII interacts with TGF beta RI to form the heteromeric signaling complex thattransduces TGF beta signals. A splice variant of the type II receptor, TGF beta RIIb, containing a 25 amino acid residue insertion near the Nterminus of the mature protein has also been described.
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