Human TGF-beta  RII Antibody

Catalog # Availability Size / Price Qty
AF-241-NA
AF-241-SP
Human TGF-beta  RII Antibody in Data
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Product Details
Citations (17)
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Human TGF-beta  RII Antibody Summary

Species Reactivity
Human
Specificity
Detects human TGF-beta RII in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RII
Ile24-Asp159
Accession # P37173.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
1 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human TGF-beta RII Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human TGF‑ beta  RII Biotinylated Antibody (Catalog # BAF241)

Standard: Recombinant Human TGF-beta RII Protein (Catalog # 241-R2)

Neutralization
Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the TF‑1 human erythroleukemic cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 5‑20 µg/mL in the presence of 0.04 ng/mL Recombinant Human TGF‑ beta 1 and 5 ng/mL Recombinant Human IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human TGF-beta  RII antibody by Western Blot. View Larger

Detection of Human TGF‑ beta RII by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for TGF-beta RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of TGF-beta  RII antibody in Human Lymphocytes antibody by Flow Cytometry. View Larger

Detection of TGF-beta RII in Human Lymphocytes by Flow Cytometry. Human blood-derived lymphocytes were labeled with Goat Anti-Human TGF-beta RII Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF-241-NA, filled histogram) or control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108).

Immunohistochemistry TGF-beta  RII antibody in Human Pituitary by Immunohistochemistry (IHC-P). View Larger

TGF-beta RII in Human Pituitary. TGF-beta RII was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Human TGF‑ beta  RII Antibody. View Larger

TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Human TGF‑ beta RII Antibody. Recombinant Human TGF-beta 1 (240-B) inhibits Recombinant Human IL-4 (204-IL) induced proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-4 (5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (0.04 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti- Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA). The ND50 is typically 10-20 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RII

TGF-beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF-beta RII interacts with TGF-beta RI to form the heteromeric signaling complex thattransduces TGF beta signals. A splice variant of the type II receptor, TGF-beta RIIb, containing a 25 amino acid residue insertion near the Nterminus of the mature protein has also been described.

Long Name
Transforming Growth Factor beta Receptor II
Entrez Gene IDs
7048 (Human); 21813 (Mouse)
Alternate Names
AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II

Product Datasheets

Citations for Human TGF-beta  RII Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Inhibition of IL-10 and TGF-? receptors on dendritic cells enhances activation of effector T-cells to kill cholangiocarcinoma cells
    Authors: C Thepmalee, A Panya, M Junking, T Chieochans, PT Yenchitsom
    Hum Vaccin Immunother, 2018;0(0):0.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. TGF-? contamination of purified recombinant GDF15
    Authors: OE Olsen, A Skjærvik, BF Størdal, A Sundan, T Holien
    PLoS ONE, 2017;12(11):e0187349.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. 24R,25-Dihydroxyvitamin D3 Protects against Articular Cartilage Damage following Anterior Cruciate Ligament Transection in Male Rats
    PLoS ONE, 2016;11(8):e0161782.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Blocking
  4. Clathrin light chains' role in selective endocytosis influences antibody isotype switching
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of alphavbeta6 integrin in DCIS identifies high-risk patients and predicts recurrence.
    Authors: Allen M, Thomas G, Clark S, Dawoud M, Vallath S, Payne S, Gomm J, Dreger S, Dickinson S, Edwards D, Pennington C, Sestak I, Cuzick J, Marshall J, Hart I, Jones J
    Clin Cancer Res, 2014;20(2):344-57.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Integrin-mediated type II TGF-beta receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling.
    Authors: Chen X, Wang H, Liao H, Hu W, Gewin L, Mernaugh G, Zhang S, Zhang Z, Vega-Montoto L, Vanacore R, Fassler R, Zent R, Pozzi A
    J Clin Invest, 2014;124(8):3295-310.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Neutralization
  7. CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGFbeta / GARP-mediated mechanism.
    Authors: Miller M, Petty C, Tompkins M, Fogle J
    Virol J, 2014;11(0):7.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Neutralization
  8. T-cell TGF-beta signaling abrogation restricts medulloblastoma progression.
    Authors: Gate D, Danielpour M, Rodriguez J, Kim G, Levy R, Bannykh S, Breunig J, Kaech S, Flavell R, Town T
    Proc Natl Acad Sci U S A, 2014;111(33):E3458-66.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Mesothelial cells promote early ovarian cancer metastasis through fibronectin secretion.
    Authors: Kenny H, Chiang C, White E, Schryver E, Habis M, Romero I, Ladanyi A, Penicka C, George J, Matlin K, Montag A, Wroblewski K, Yamada S, Mazar A, Bowtell D, Lengyel E
    J Clin Invest, 2014;124(10):4614-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells.
    Authors: Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F
    Cancer Res, 2013;73(7):2333-44.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis.
    Authors: Rai D, Kim SW, McKeller MR
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3111-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Antibody-mediated blockade of integrin alpha v beta 6 inhibits tumor progression in vivo by a transforming growth factor-beta-regulated mechanism.
    Authors: Van Aarsen LA, Leone DR, Ho S, Dolinski BM, McCoon PE, LePage DJ, Kelly R, Heaney G, Rayhorn P, Reid C, Simon KJ, Horan GS, Tao N, Gardner HA, Skelly MM, Gown AM, Thomas GJ, Weinreb PH, Fawell SE, Violette SM
    Cancer Res., 2008;68(2):561-70.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Tumor-induced anorexia and weight loss are mediated by the TGF-beta superfamily cytokine MIC-1.
    Authors: Johnen H, Lin S, Kuffner T, Brown DA, Tsai VW, Bauskin AR, Wu L, Pankhurst G, Jiang L, Junankar S, Hunter M, Fairlie WD, Lee NJ, Enriquez RF, Baldock PA, Corey E, Apple FS, Murakami MM, Lin EJ, Wang C, During MJ, Sainsbury A, Herzog H, Breit SN
    Nat. Med., 2007;13(11):1333-40.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. Very low O2 concentration (0.1%) favors G0 return of dividing CD34+ cells.
    Authors: Hermitte F, Brunet de la Grange P, Belloc F, Praloran V, Ivanovic Z
    Stem Cells, 2005;24(1):65-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2005;105(2):750-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  16. TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing.
    Authors: Song QH, Klepeis VE, Trinkaus-Randall V
    MP, Mol. Pathol., 2002;55(3):164-76.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  17. Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways.
    Authors: Rosado E, 2019, Schwartz Z, Sylvia VL, Dean DD, Boyan BD
    91, 2002;1590(1):1-15.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization

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