Mouse B7-1/CD80 Antibody

Catalog # Availability Size / Price Qty
AF740
AF740-SP
Detection of Mouse B7‑1/CD80 by Western Blot.
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Product Details
Citations (6)
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Supplemental Products
Reviews (2)

Mouse B7-1/CD80 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse B7‑1/CD80 in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7‑1/CD80
Asp37-Lys245
Accession # Q00609
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Mouse B7-1/CD80 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse B7‑1/CD80 Biotinylated Antibody (Catalog # BAF740)

Standard: Recombinant Mouse B7-1/CD80 Fc Chimera His-tag Protein, CF (Catalog # 740-B1)

Neutralization
Measured by its ability to neutralize B7‑1/CD80-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse B7‑1/CD80 Fc Chimera and 10 µg/mL PHA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse B7‑1/CD80 by Western Blot. View Larger

Detection of Mouse B7‑1/CD80 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7-1/CD80 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of B7‑1/CD80 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of B7‑1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either treated with 200 ng/mL LPS (filled histogram) or unstimulated (open histogram) were stained with Goat Anti-Mouse B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of B7‑1/CD80 in Mouse Splenocytes by Flow Cytometry View Larger

Detection of B7‑1/CD80 in Mouse Splenocytes by Flow Cytometry Mouse B6 splenocytes treated with 200 ng/mL LPS for 48 hr were stained with (A) Goat Anti-Mouse B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740) or (B) Goat IgG control antibody (AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107) and Rat Anti-Mouse B220/CD45R Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB1217G). View our protocol for Staining Membrane-associated Proteins.

Neutralization IL‑2 secretion Induced by B7‑1/CD80 and Neutralization by Mouse B7‑1/CD80 Antibody. View Larger

IL‑2 secretion Induced by B7‑1/CD80 and Neutralization by Mouse B7‑1/CD80 Antibody. Recombinant Mouse B7-1/CD80 Fc Chimera (Catalog # 740-B1) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Mouse B7-1/CD80 Fc Chimera (0.1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740). The ND50 is typically 0.15-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-1/CD80

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the
down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Mouse B7-1 is a 306 amino acid (aa) protein containing a putative 37 aa signal peptide, a 190 aa extracellular domain, a 22 aa transmembrane domain, and a 38 aa cytoplasmic domain. Mouse B7-1 and B7-2 share 28% amino acid identity. Mouse and human B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse
B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

References
  1. Azuma, M. et al. (1993) Nature 366:76.
  2. Freeman, G.J. et al. (1993) Science 262:909.
  3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
  5. Chen, C. et al. (1994) J. Immunol. 152:4929.
  6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Entrez Gene IDs
941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

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Citations for Mouse B7-1/CD80 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Dysregulation of Immune Response Mediators and Pain-Related Ion Channels Is Associated with Pain-like Behavior in the GLA KO Mouse Model of Fabry Disease
    Authors: M Spitzel, E Wagner, M Breyer, D Henniger, M Bayin, L Hofmann, D Mauceri, C Sommer, N Üçeyler
    Cells, 2022;11(11):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Resveratrol Ameliorates Cardiac Remodeling in a Murine Model of Heart Failure With Preserved Ejection Fraction
    Authors: L Zhang, J Chen, L Yan, Q He, H Xie, M Chen
    Frontiers in Pharmacology, 2021;12(0):646240.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. High fat diet and leptin promote tumor progression by inducing myeloid-derived suppressor cells
    Authors: VK Clements, T Long, R Long, C Figley, DMC Smith, S Ostrand-Ro
    J. Leukoc. Biol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Aging is associated with increased regulatory T-cell function.
    Authors: Garg S, Delaney C, Toubai T, Ghosh A, Reddy P, Banerjee R, Yung R
    Aging Cell, 2014;13(3):441-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.
    Authors: Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S
    Eur. J. Immunol., 2009;39(1):85-95.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract.
    Authors: He H, Li W, Chen SY, Zhang S, Chen YT, Hayashida Y, Zhu YT, Tseng SC
    Invest. Ophthalmol. Vis. Sci., 2008;49(10):4468-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Mouse B7-1/CD80 Antibody

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Mouse B7-1/CD80 Antibody
By Anonymous on 12/12/2021
Application: ICC/IF Sample Tested: Lung tissue Species: Mouse

Mouse B7-1/CD80 Antibody
By Anonymous on 12/18/2018
Application: Flow Sample Tested: Pancreas tissue Species: Mouse