Mouse IL-1 alpha /IL-1F1 Antibody

Name: Mouse IL-1 alpha /IL-1F1 Antibody
Brand: R&D Systems
Rating: 4.7 (3 reviews)

(11 citations)

(3 Reviews)

Datasheet / CoA / SDS

Western Blot

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Immunohistochemistry

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Neutralization

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Product Details

Citations (11)

FAQs

Supplemental Products

Reviews

Species Reactivity

Mouse
Specificity

Detects mouse IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat IL‑1 alpha and recombinant cotton rat IL‑1 alpha is observed and less than 1% cross-reactivity with recombinant human IL‑1 alpha is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

E. coli-derived recombinant mouse IL‑1 alpha /IL‑1F1
Ser6-Ser161
Accession # Q62161
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

0.1 µg/mL

See below
Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.002-0.004 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot click image to view larger	Detection of Recombinant Mouse and Rat IL‑1 alpha /IL‑1F1 by Western Blot. Western blot shows 50 ng of Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400‑ML), Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) and Recombinant Rat IL‑1 alpha /IL‑1F1 (Catalog # 500-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑400‑NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑1 alpha /IL‑1F1 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunohistochemistry click image to view larger	IL‑1 alpha /IL‑1F1 in Mouse Liver. IL‑1 alpha /IL‑1F1 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neutralization click image to view larger	Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Mouse IL‑1 alpha /IL‑1F1 Antibody. Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF-400-NA). The ND₅₀ is typically 0.002-0.004 µg/mL in the presence of concanavalin A (1.25 µg/mL).

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.

Long Name:

Interleukin 1 alpha

Entrez Gene IDs:

3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)

Alternate Names:

Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species	Applications	Sample Type
Tacrolimus interacts with voriconazole to reduce the severity of fungal keratitis by suppressing IFN-related inflammatory responses and concomitant FK506 and voriconazole treatment suppresses fungal keratitis Authors: J Zhong, L Peng, B Wang, H Zhang, S Li, R Yang, Y Deng, H Huang, J Yuan Mol. Vis., 2018;24(0):187-200. Species: Mouse Sample Type: Cell Lysates Application: WB Interleukin-1 and estrogen protect against disseminating dentoalveolar infections Authors: H Youssef, P Stashenko Int J Oral Sci, 2017;9(1):16-23. Species: Mouse Sample Type: In Vivo Application: In vivo TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects Authors: MK Choo, Y Sano, C Kim, K Yasuda, XD Li, X Lin, M Stenzel-Po, L Alexopoulo, S Ghosh, E Latz, IR Rifkin, ZJ Chen, GC Stewart, H Chong, JM Park J. Exp. Med., 2017;214(5):1297-1311. Species: Mouse Sample Type: Cell Lysates Application: WB AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3. Authors: Denes A, Coutts G, Lenart N, Cruickshank S, Pelegrin P, Skinner J, Rothwell N, Allan S, Brough D Proc Natl Acad Sci U S A, 2015;112(13):4050-5. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen IL-1alpha signaling is critical for leukocyte recruitment after pulmonary Aspergillus fumigatus challenge. Authors: Caffrey A, Lehmann M, Zickovich J, Espinosa V, Shepardson K, Watschke C, Hilmer K, Thammahong A, Barker B, Rivera A, Cramer R, Obar J PLoS Pathog, 2015;11(1):e1004625. Species: Mouse Sample Type: In Vivo Application: In vivo Dendritic cell IL-1alpha and IL-1beta are polyubiquitinated and degraded by the proteasome. Authors: Ainscough J, Frank Gerberick G, Zahedi-Nejad M, Lopez-Castejon G, Brough D, Kimber I, Dearman R J Biol Chem, 2014;289(51):35582-92. Species: Mouse Sample Type: Cell Lysates Application: WB Interleukin-1 deficiency prolongs ovarian lifespan in mice. Authors: Uri-Belapolsky S, Shaish A, Eliyahu E, Grossman H, Levi M, Chuderland D, Ninio-Many L, Hasky N, Shashar D, Almog T, Kandel-Kfir M, Harats D, Shalgi R, Kamari Y Proc Natl Acad Sci U S A, 2014;111(34):12492-7. Species: Mouse Sample Type: Tissue Homogenates Application: WB The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. Authors: Asrat S, Dugan A, Isberg R PLoS Pathog, 2014;10(7):e1004229. Species: Mouse Sample Type: Cell Lysates Application: WB IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation. Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN J. Immunol., 2011;187(9):4835-43. Species: Mouse Sample Type: Cell Lysates Application: WB Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes. Authors: Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA Nat. Immunol., 2010;11(10):897-904. Species: Mouse Sample Type: Cell Lysates Application: WB Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening. Authors: Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM Bone, 2007;40(5):1276-83. Species: Mouse Sample Type: Whole Cells Application: Neut Expand to show all Citations

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Cell and Tissue Staining Kits

Description Application Cat# Citations Images

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 17

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Mouse IL-1 alpha /IL-1F1 Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

Antigen Retrieval Reagent-Universal

Antigen Retrieval Reagent-Basic

Mounting Medium

Antigen Retrieval Reagent Sampler (50 mL each)

Antigen Retrieval Reagent-Acidic

VisUCyte Antigen Retrieval Reagent-Basic

VisUCyte Antigen Retrieval Reagent-Universal

NorthernLights Guard Mounting Media

VisUCyte Antigen Retrieval Reagent-Acidic

Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

Goat IgG HRP-conjugated Antibody

Goat IgG HRP-conjugated Antibody

Goat IgG (H+L) PE-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Goat IgG Horseradish Peroxidase-conjugated Antibody

Goat IgG (H+L) APC-conjugated Antibody

Donkey Anti-Goat IgG Antibody

Goat IgG VisUCyte HRP Polymer

Donkey Anti-Goat IgG Biotinylated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Goat IgG (H+L) Fluorescein-conjugated Antibody

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

Chicken Anti-Goat IgG Biotinylated Antibody

Rabbit Anti-Goat IgG Biotinylated Antibody

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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