Mouse/Rat IFN-gamma Antibody

Catalog # Availability Size / Price Qty
AF-585-NA
AF-585-SP
IFN‑ gamma  Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse/Rat IFN‑ gamma  Antibody.
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Product Details
Citations (4)
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Mouse/Rat IFN-gamma Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects rat and mouse IFN-gamma in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat IFN-gamma
Gln23-Cys156
Accession # P01581
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat IFN-gamma (Catalog # 585-IF)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.08-0.2 µg/mL in the presence of 2.5 ng/mL Recombinant Rat IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN‑ gamma  Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse/Rat IFN‑ gamma  Antibody. View Larger

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse/Rat IFN‑ gamma Antibody. Recombinant Rat IFN-gamma (Catalog # 585-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rat IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA). The ND50 is typically 0.08-0.2 µg/mL.

Immunocytochemistry IFN-? antibody in Rat Splenocytes by Immunocytochemistry (ICC). View Larger

IFN‑ gamma in Rat Splenocytes. IFN-gamma was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Goat Anti-Mouse/Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rat IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with mouse IFN-gamma and 37-45% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus macaque IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Dijkema, R. et al. (1985) EMBO J. 4:761.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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Citations for Mouse/Rat IFN-gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Expression of pro-inflammatory cytokines in the auditory cortex of rats with salicylate-induced tinnitus
    Authors: XH Chen, LL Zheng
    Mol Med Rep, 2017-08-11;0(0):.
  2. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
    Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
    Sci Rep, 2017-04-20;7(0):46514.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
    Authors: Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K
    J Neurosci, 2014-02-05;34(6):2231-43.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Ultraviolet B suppresses immunity by inhibiting effector and memory T cells.
    Authors: Rana S, Byrne SN, MacDonald LJ, Chan CY, Halliday GM
    Am. J. Pathol., 2008-02-21;172(4):993-1004.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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