Mouse TNF RII/TNFRSF1B Antibody

(7 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TNF RII in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TNF RII is observed and less than 1% cross-reactivity with recombinant mouse (rm) CD27, rmGITR, rhDR6, rmCD30, rmCD40, rmEDAR, rmFas, rmOPG, rmRANK, and rmTNF RI is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse TNF RII/TNFRSF1B
    Val23-Gly258
    Accession # P25119
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed mouse splenocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse TNF RII/TNFRSF1B by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RII/TNFRSF1B at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of TNF RII/TNFRSF1B in L‑929 Mouse Cell Line by Flow Cytometry.

L‑929 mouse fibroblast cell line was stained with Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RII/TNFRSF1B
Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta. These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A. In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions. Although the physiological role of these proteins is not known, it has been speculated that shedding of the soluble receptors in response to TNF release could serve as a mechanism to scavenge the TNF not immediately bound and thus localize the inflammatory response. It is also possible that the pool of TNF bound to soluble receptors could represent a reservoir for the controlled release of TNF.
  • Long Name:
    Tumor Necrosis Factor Receptor II
  • Entrez Gene IDs:
    7133 (Human); 21938 (Mouse); 156767 (Rat)
  • Alternate Names:
    CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Endogenous TNF? orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation
    Authors: S Arokiasamy, C Zakian, J Dilliway, W Wang, S Nourshargh, MB Voisin
    Sci Rep, 2017;7(0):44189.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2
    Authors: R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann
    Sci Rep, 2017;7(1):6607.
    Species: Mouse
    Sample Type: Whole Cells
  3. Phenotypic screening reveals TNFR2 as a promising target for cancer immunotherapy
    Oncotarget, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Myosin light chain kinase expression induced via tumor necrosis factor receptor 2 signaling in the epithelial cells regulates the development of colitis-associated carcinogenesis.
    Authors: Suzuki M, Nagaishi T, Yamazaki M, Onizawa M, Watabe T, Sakamaki Y, Ichinose S, Totsuka M, Oshima S, Okamoto R, Shimonaka M, Yagita H, Nakamura T, Watanabe M
    PLoS ONE, 2014;9(2):e88369.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients.
    Authors: Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S
    J Immunol, 2014;193(4):2024-33.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
    Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S
    J. Exp. Med., 2012;209(6):1219-34.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
    Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
    J. Clin. Invest., 2009;119(10):2954-64.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
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