PDX-1/IPF1 Antibody (267712)

R&D Systems | Catalog # MAB2419

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 267712
View all PDX-1/IPF1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PDX-1
Ala91-Arg283
Accession # P52945

Specificity

Detects human and mouse PDX-1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for PDX-1/IPF1 Antibody (267712)

Detection of Mouse PDX-1/IPF1 antibody by Western Blot.

Detection of Mouse PDX‑1/IPF1 by Western Blot.

Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDX-1/IPF1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PDX-1/IPF1 antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC).

PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line.

PDX-1/IPF1 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
PDX-1/IPF1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells.

PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Detection of PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line by Flow Cytometry.

beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Detection of PDX‑1/IPF1 in Human Pancreas.

PDX‑1/IPF1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human/Mouse PDX‑1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus in islet cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of PDX-1/IPF1 by Immunohistochemistry

Detection of PDX-1/IPF1 by Immunohistochemistry

Cell-of-origin analysis using normal cell type methylation profiles and SOX9 in PanNEN subgroups indicate exocrine lineage for Group B tumors. a (i) Phylo-epigenetic analysis of PanNEN tumors and normal pancreatic cell types. Pearson distance between the samples computed using differentially methylated CpGs between normal alpha -, beta -, ductal and acinar cells (n = 46,500, adj. p value < 0.01 and | delta beta | > 0.2) and neighbor-joining tree estimation. (ii) IHC of outliers PNET4 and PNET 60, which were re-assessed and removed from phylo-epigenetic analysis (i). (iii) New phylo-epigenetic analysis without PNET4 and PNET60. b Euclidean distance between each cell type was computed and correlation matrix of the distances is displayed. Heatmap depicts the distance between a given normal cell-type pair. c Boxplot representing distribution of the proportion of atlas signatures of alpha -, beta -, ductal and acinar cells (each main box) in the subgroups and PDACs; each dot depicts the proportion of atlas signatures of the respective cell type in a given sample. Two-sample Wilcoxon test. d IHC of SOX9. Representative images for each subgroup; scale bar: 20μm. Table depicts the total IHC score for Group A and Group B samples Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35227293), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for PDX-1/IPF1 Antibody (267712)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed beta TC-6 mouse beta cell insulinoma cell line and BG01V human embryonic stem cells differentiated into pancreatic progenitor cells

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: beta TC-6 mouse beta cell insulinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Western Blot

1 µg/mL
Sample: beta TC-6 mouse beta cell insulinoma cell line

Reviewed Applications

Read 3 reviews rated 4.3 using MAB2419 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDX-1/IPF1

Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomain transcription factor essential for pancreatic development and mature pancreatic beta cell function.

Long Name

Pancreas/Duodenum Homeobox-1

Alternate Names

IDX1, IPF1

Entrez Gene IDs

3651 (Human); 18609 (Mouse)

Gene Symbol

PDX1

UniProt

P52945

Additional PDX-1/IPF1 Products

Product Documents for PDX-1/IPF1 Antibody (267712)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PDX-1/IPF1 Antibody (267712)

For research use only

Citations for PDX-1/IPF1 Antibody (267712)

Customer Reviews for PDX-1/IPF1 Antibody (267712) (3)

4.3 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse PDX-1/IPF1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/01/2026
    Whole cell lysate of human adipocytes and MCF7 coculture
    PDX-1/IPF1 Antibody (267712) MAB2419
  • Human/Mouse PDX-1/IPF1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 01/01/2026
    Used at 1 ug/mL
    PDX-1/IPF1 Antibody (267712) MAB2419
  • Human/Mouse PDX-1/IPF1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: BG01V human embryonic stem cells
    Species: Human
    Verified Customer | Posted 02/11/2022
    PDX-1/IPF1 Antibody (267712) MAB2419

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Protocols

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