B7-CD28 Family of Immune Checkpoint Proteins

B7-CD28 immune checkpoint proteins

Members of the B7:CD28 family represent the most widely studied immune checkpoint regulators. The ability of cancer cells to develop mechanisms to evade the host’s immune system often involves overexpression of certain immune checkpoint proteins. These proteins recognize and bind to receptors on immune cells that initiate a negative signaling cascade allowing the tumor to go on living undetected. However, this clever cloaking device used by cancer cells has opened up an exciting new opportunity to fight cancer. Nobel Prize winning discoveries by James Allison and Tasuku Honjo for their breakthrough discoveries in cancer immunotherapy have sparked great interest in the field. They showed that by targeting and disrupting these protein-protein interactions, the immune system can now recognize and attack the tumor. Blocking antibodies that specifically disrupt these protein-protein interactions have produced remarkable results in certain cancers. So far, three cancer immunotherapies have been approved by the FDA, Ipilimumab (anti-CTLA-4, Bristol-Myers Squibb), nivolumab (anti-PD-1, Bristol-Myers Squibb), and pembrolizumab (anti-PD-1, Merck), with many more in clinical trials. Several new promising targets have been identified that exhibit inhibitory effects on immune cells similar to PD-1:PD-L1. These next generation proteins may unlock new opportunities in the fight against cancer.

 

In addition to the well-known immune checkpoint proteins PD-1, PD-L1, CTLA-4, and CD28, there are many more cell surface proteins that exhibit similar immunomodulatory behavior. The B7 family now comprises 10 members, B7-1/CD80, B7-2/CD86, PD-L1/B7-H1, PD-L2/B7-DC, B7-H2, B7-H3, B7-H4, B7-H5/VISTA/PD-1H, B7-H6, and B7-H7/HHLA2. As the world’s leading recombinant protein company, R&D Systems exclusively offers a comprehensive range of bioactive B7:CD28 proteins in a variety of different species as well as an expanding line of Avi-tag biotinylated proteins.

T-Cell Co-Signaling

B7:CD28 Family Proteins

QC Tested for Bioactivity

Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF Bioactivity

Recombinant Human PD-L1/B7-H1 Fc Chimera (Catalog # 156-B7) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.075-0.75 μg/mL in the presence of Goat Anti-Human IgG Fc Polyclonal Antibody (Catalog # G-102-C).

Recombinant Human CTLA-4 Fc Chimera (CHO-expressed), CF Bioactivity

Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL when stimulated with 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).

Recombinant Human B7-1/CD80 Fc Chimera Protein, CF Bioactivity

Recombinant Human B7‑1/CD80 Fc Chimera (Catalog #10133-B1) induces IL-2 secretion by Jurkat human acute T cell leukemia cells in the presence of PHA. The ED50 for this effect is 0.025-0.15 µg/mL.

 

Protein-Protein Interaction Data

Recombinant Human PD-1 Fc Chimera Protein, CF Bioactivity

When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is coated at 0.1 μg/mL, Recombinant Human B7-H1/PD‑L1 Fc Chimera (Catalog # 156-B7) binds with a typical ED50 of 0.15-0.75 µg/mL.

Recombinant Human VISTA/B7-H5/PD-1H His Avi-tag Protein, CF Binding Activity

When serially diluted Biotinylated Recombinant Human VISTA/B7-H5/PD-1H His-tag Avi-tag Protein (Catalog # AVI9057) is immobilized onto an Even Coat Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) with an ED50 of 75-450 ng/mL.

Recombinant Human PD-L2/B7-DC His-tag Avi-tag Protein, CF Binding Activity

When Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD) is immobilized at 1 µg/mL, Biotinylated Recombinant Human PD-L2/B7-DC His-tag Avi-tag (Catalog # AVI9075) binds with an ED50 of 0.2-1.6 µg/mL.

 

SDS-PAGE

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF SDS-PAGE

1 µg/lane of Recombinant Mouse PD-L1/B7-H1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 79 kDa and 150 kDa, respectively.

Recombinant Human B7-1/CD80 Fc Chimera Avi-tag Protein, CF SDS-PAGE

2 µg/lane of Recombinant Human B7-1/CD80 Fc Chimera (Catalog # AVI10107) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-89 kDa and 150-180 kDa, respectively.

Recombinant Cynomolgus B7-H7/HHLA2 Fc Chimera Protein, CF SDS-PAGE

1 µg/lane of Recombinant Cynomolgus Monkey B7-H7/HHLA2 Fc Chimera (Catalog # 10108-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 92-109 kDa and 180-220 kDa, respectively.