Human EMMPRIN/CD147 Antibody
Human EMMPRIN/CD147 Antibody Summary
Ala22-His205
Accession # Q54A51
Applications
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human EMMPRIN/CD147 DuoSet ELISA Kit (Catalog # DY972) for convenient development of a sandwich ELISA or the Human EMMPRIN/CD147 Quantikine ELISA Kit (Catalog # DEMP00) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human EMMPRIN/CD147 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB972) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EMMPRIN/CD147 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot Shows Human EMMPRIN/CD147 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and EMMPRIN/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB972) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EMMPRIN/CD147 at approximately 51 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Human EMMPRIN/CD147 ELISA Standard Curve. Recombinant Human EMMPRIN/CD147 protein was serially diluted 2-fold and captured by Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB972) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (AF972) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EMMPRIN/CD147
EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily. It is a multifunctional protein involved in reproduction, neural function, inflammation and tumor invasion.
Product Datasheets
Citations for Human EMMPRIN/CD147 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Curcumin inhibits adverse psychological stress-induced proliferation and invasion of glioma cells via down-regulating the ERK/MAPK pathway
Authors: P Wang, X Hao, X Li, Y Yan, W Tian, L Xiao, Z Wang, J Dong
Journal of Cellular and Molecular Medicine, 2021;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: Western Blot -
CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts
Authors: M Aoki, K Koga, M Miyazaki, M Hamasaki, N Koshikawa, M Oyama, H Kozuka-Hat, M Seiki, BP Toole, K Nabeshima
BMC Cancer, 2019;19(1):912.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The net acid extruders NHE1, NBCn1 and MCT4 promote mammary tumor growth through distinct but overlapping mechanisms
Authors: AP Andersen, J Samsøe-Pet, EK Oernbo, E Boedtkjer, JMA Moreira, M Kveiborg, SF Pedersen
Int. J. Cancer, 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Membrane type 1 matrix metalloproteinase (MT1-MMP/MMP-14) cleaves and releases a 22-kDa extracellular matrix metalloproteinase inducer (EMMPRIN) fragment from tumor cells.
Authors: Egawa N, Koshikawa N, Tomari T, Nabeshima K, Isobe T, Seiki M
J. Biol. Chem., 2006;281(49):37576-85.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Immunolocalization of EMMPRIN (CD147) in the human eye and detection of soluble form of EMMPRIN in ocular fluids.
Authors: Määttä M, Tervahartiala T, Kaarniranta K, Tang Y, Yan L, Tuukkanen J, Sorsa T
Curr. Eye Res., 2006;31(11):917-24.
Species: Human
Sample Types: Tears
Applications: Western Blot -
Oxidized low-density lipoproteins stimulate extracellular matrix metalloproteinase Inducer (EMMPRIN) release by coronary smooth muscle cells.
Authors: Haug C, Lenz C, Diaz F, Bachem MG
Arterioscler. Thromb. Vasc. Biol., 2004;24(10):1823-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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