Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, ELISA, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, Immunoprecipitation, ELISA Capture, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EMMPRIN/CD147 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EMMPRIN/CD147
Thr25-His205
Accession # Q54A51

Specificity

Detects human EMMPRIN/CD147 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse EMMPRIN is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human EMMPRIN/CD147 Antibody

Detection of Human EMMPRIN/CD147 antibody by Western Blot.

Detection of Human EMMPRIN/CD147 by Western Blot.

Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EMMPRIN/CD147 at approximately 38-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EMMPRIN/CD147 antibody in Human Stomach Cancer Tissue by Immunohistochemistry (IHC-P).

EMMPRIN/CD147 in Human Stomach Cancer Tissue.

EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Goat Anti-Human EMMPRIN/ CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

EMMPRIN/CD147 antibody in Human Cervix by Immunohistochemistry (IHC-P).

EMMPRIN/CD147 in Human Cervix.

EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human cervix using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human EMMPRIN/CD147 antibody by Simple WesternTM.

Detection of Human EMMPRIN/CD147 by Simple WesternTM.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 70 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human EMMPRIN/CD147 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human EMMPRIN/CD147 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and EMMPRIN/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 51 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human EMMPRIN/CD147 ELISA Standard Curve.

Recombinant Human EMMPRIN/CD147 protein was serially diluted 2-fold and captured by Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (MAB972) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Detection of Human EMMPRIN/CD147 by Western Blot

Detection of Human EMMPRIN/CD147 by Western Blot

The proximal Panx1CT is necessary for cell surface localization of Panx1-EGFP. (b) Cell surface biotinylation assays reveal that the proximal Panx1CT is required for cell surface localization in HEK293T cells. (i) Representative Western blot of pulldown (cell surface protein) and input. Anti-EMMPRIN was used as a positive control for biotin pulldown and as a loading control, and anti-GAPDH was used as a negative control against biotin internalization. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31278290), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human EMMPRIN/CD147 by Western Blot

Detection of Human EMMPRIN/CD147 by Western Blot

A novel putative LRR motif in the Panx1CT is necessary for trafficking Panx1-EGFP to the cell surface. (a) Schematic of full length Panx1-EGFP and the Panx1∆LRR-EGFP, Panx1∆HCS-EGFP, and Panx1∆VS-EGFP deletion mutants. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31278290), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human EMMPRIN/CD147 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB972).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human EMMPRIN/CD147 DuoSet ELISA Kit (Catalog # DY972) for convenient development of a sandwich ELISA or the Human EMMPRIN/CD147 Quantikine ELISA Kit (Catalog # DEMP00) for  a complete optimized ELISA.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast, gastric, lung, stomach, pancreatic cancer tissue, and cervix

Knockout Validated

EMMPRIN/CD147 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in EMMPRIN/CD147 knockout HEK293T cell line.

Simple Western

10 µg/mL
Sample: Human brain (cerebellum) tissue

Western Blot

1 µg/mL
Sample: Human brain tissue

Reviewed Applications

Read 1 review rated 4 using AF972 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EMMPRIN/CD147

Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 44-66 kDa, variably glycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily (1-4). Human EMMPRIN is 269 amino acids (aa) in length and contains a 24 aa signal sequence, a 183 aa extracellular domain (ECD), a 21 aa transmembrane (TM) domain and a 41 aa intracellular domain. The ECD contains one C2-type and one V-type Ig-like domain. There is one 385 aa splice variant that contains an extra N-terminal IgCAM domain and is found only in the retina (5). mRNA transcripts, but not protein, have been reported for additional 432, 388, 205, 176, and 174 aa variants.

EMMPRIN is expressed in areas of tissue remodeling, including tumors, endometrium, placenta, skin, and regions undergoing angiogenesis (1, 2, 6-9). It is also expressed in cells with high metabolic activity, such as lymphoblasts, macrophages and tumor cells (2, 10). On cells with elevated metabolic rates, EMMPRIN is often co-expressed with the amino acid transporter CD98h (11). EMMPRIN also interacts with caveolin-1 (via its C2-like domain), and this reduces the level of EMMPRIN glycosylation and subsequent EMMPRIN multimerization and activity (12). EMMPRIN’s TM sequence contains a Glu and a Pro which are important for intracellular interactions with cyclophilins (CyP) (3, 13, 14). CyPA (cyclosporin A receptor) and CyP60 interactions with the TM segment promote leukocyte inflammatory chemotaxis and surface expression of EMMPRIN, respectively (13, 14). An active 22 kDa fragment can be shed from tumor cells by MT1-MMP (1). Tumor cells can also release active, full-length EMMPRIN in microvesicles (15, 16). Functionally, EMMPRIN is known to induce urokinase-type plasminogen activator (uPA), VEGF, hyaluronan, and multiple MMPs (1, 2, 7, 8, 9). Human EMMPRIN (269 aa) shows 58%, 58%, 62%, and 52% aa identity with mouse, rat, bovine, and chicken EMMPRIN, respectively. It also shows 25% and 38% aa identity with the related proteins, embigin and neuroplastin (SDR-1), respectively (4).

References

  1. Gabison, E.E. et al. (2005) Biochimie 87:361.
  2. Yurchenko, V. et al. (2006) Immunology 117:301.
  3. Kasinrerk, W. et al. (1992) J. Immunol. 149:847.
  4. Miyauchi, T. et al. (1991) J. Biochem. 110:770.
  5. Hanna, S.M. et al. (2003) BMC Biochem. 4:17.
  6. Riethdorf, S. et al. (2006) Int. J. Cancer 119:1800.
  7. Braundmeier, A.G. et al. (2006) J. Clin. Endocrinol. Metab. 91:2358.
  8. Tang, Y. et al. (2006) Mol. Cancer Res. 4:371.
  9. Quemener, C. et al. (2007) Cancer Res. 67:9.
  10. Wilson, M.C. et al. (2005) J. Biol. Chem. 280:27213.
  11. Xu, D. and M.E. Hemler (2005) Mol. Cell. Proteomics 4:1061.
  12. Tang, W. et al. (2004) Mol. Biol. Cell 15:4043.
  13. Arora, K. et al. (2005) J. Immunol. 175:517.
  14. Pushkarsky, T. et al. (2005) J. Biol. Chem. 280:27866.
  15. Egawa, N. et al. (2006) J. Biol. Chem. 281:37576.
  16. Sidhu, S.S. et al. (2004) Oncogene 23:956.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Entrez Gene IDs

682 (Human); 12215 (Mouse); 102121721 (Cynomolgus Monkey)

Gene Symbol

BSG

UniProt

Q54A51

Additional EMMPRIN/CD147 Products

Product Documents for Human EMMPRIN/CD147 Antibody

Certificate of Analysis

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Product Specific Notices for Human EMMPRIN/CD147 Antibody

For research use only

Citations for Human EMMPRIN/CD147 Antibody

Customer Reviews for Human EMMPRIN/CD147 Antibody (1)

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    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 24527394
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