Human/Mouse/Rat Park7/DJ‑1 Antibody

Human Park7/DJ‑1 ELISA Standard Curve.

Recombinant Human Park7/DJ-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39952) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human/Mouse/Rat Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39951) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Western Blot Shows Park7/DJ‑1 Specificity Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7/DJ‑1 knockout HEK293T cell line (KO). Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse/Rat Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB39951) followed by HRP-conjugated secondary antibody. A specific band was detected for Park7/DJ‑1 at approximately 20 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. Primary antibody dilution used: 1 µg/mL. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Park7/DJ‑1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

HEK293T human embryonic kidney parental cell line WT and Park7/DJ‑1 HEK293T KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Mouse Anti-Human/Mouse/Rat Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB39951) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).