Detection of Human, Mouse, and Rat Park7/DJ‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MEF mouse embryonic feeder cells, and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB39951) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Park7/DJ‑1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Park7/DJ‑1 in HeLa Human Cell Line. Park7/DJ‑1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB39951) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the
ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function
as protein chaperones, catalases, proteases and kinases. Park7 is
widely expressed in the brain as well as in peripheral tissues. It
exists as a homodimer that can be localized in the cytoplasm, nucleus
and mitochondria. Park7 is a redox-sensitive protein that has been
ascribed various functions including that as a redox sensor and
antioxidant protein. Mutations in Park7 are associated with a small
percentage of hereditary early onset Parkinson’s disease. Human and
mouse Park7 share 92% amino acid sequence identity.
Parkinson Disease 7
Entrez Gene IDs:
11315 (Human); 57320 (Mouse); 117287 (Rat)
DJ1; DJ-1; DJ1FLJ34360; EC 3.4; FLJ27376; FLJ92274; Oncogene DJ1; Park7; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; protein DJ-1
Have you used Human/Mouse/Rat Park7/DJ-1 Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.