Human/Mouse/Rat Serum Albumin Antibody MAB1455: R&D Systems

Human/Mouse/Rat Serum Albumin Antibody

(15 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human Serum Albumin in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 188835
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human Serum Albumin
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2-1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Albumin by Western Blot. Western blot shows lysates of human plasma, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Albumin by Western Blot. Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/
Rat Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Albumin in Hepatocytes Derived from Human Embryonic Stem Cells.
Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human/Mouse/Rat Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
Detection of Albumin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat Serum Albumin Monoclonal Antibody (Catalog # MAB1455, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Human Albumin by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Serum Albumin Monoclonal Antibody (Catalog # MAB1455). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Albumin
Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.
  • Entrez Gene IDs:
    213 (Human); 11657 (Mouse); 24186 (Rat)
  • Alternate Names:
    ALB; Albumin; cell growth inhibiting protein 42; DKFZp779N1935; growth-inhibiting protein 20; PRO0883; PRO0903; PRO1341; serum albumin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Extracellular vesicles from human liver stem cells restore argininosuccinate synthase deficiency
    Authors: MB Herrera Sa, S Previdi, S Bruno, V Fonsato, MC Deregibus, S Kholia, S Petrillo, E Tolosano, R Critelli, M Spada, R Romagnoli, M Salizzoni, C Tetta, G Camussi
    Stem Cell Res Ther, 2017;8(1):176.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Nucleic acid-based polymers effective against hepatitis B Virus infection in patients don't harbor immunostimulatory properties in primary isolated liver cells
    Authors: CI Real, M Werner, A Paul, G Gerken, JF Schlaak, A Vaillant, R Broering
    Sci Rep, 2017;7(0):43838.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model
    Stem Cells Dev, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC - Fresh
  4. A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood
    Nat Commun, 2016;7(0):13363.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  5. Fatty acid-binding proteins (FABPs) are intracellular carriers for Delta9-tetrahydrocannabinol (THC) and cannabidiol (CBD).
    Authors: Elmes M, Kaczocha M, Berger W, Leung K, Ralph B, Wang L, Sweeney J, Miyauchi J, Tsirka S, Ojima I, Deutsch D
    J Biol Chem, 2015;290(14):8711-21.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  6. Differences in the Epigenetic Regulation of Cytochrome P450 Genes between Human Embryonic Stem Cell-Derived Hepatocytes and Primary Hepatocytes.
    Authors: Park H, Choi Y, Kim J, Chun H, Im I, Yoon S, Han Y, Song C, Kim H
    PLoS ONE, 2015;10(7):e0132992.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Identification of transcription factors for lineage-specific ESC differentiation.
    Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
    Stem Cell Reports, 2013;1(6):545-59.
  8. Production of hepatocyte-like cells from human pluripotent stem cells.
    Authors: Hannan, Nicholas, Segeritz, Charis-P, Touboul, Thomas, Vallier, Ludovic
    Nat Protoc, 2013;8(2):430-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Induction of neuronal death by microglial AGE-albumin: implications for Alzheimer's disease.
    Authors: Byun K, Bayarsaikhan E, Kim D, Kim CY, Mook-Jung I, Paek SH, Kim SU, Yamamoto T, Won MH, Song BJ, Park YM, Lee B
    PLoS ONE, 2012;7(5):e37917.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
    Authors: Hoang S, Liauw J, Choi M
    J. Cereb. Blood Flow Metab., 2009;29(2):385-97.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  11. Fate-mapping evidence that hepatic stellate cells are epithelial progenitors in adult mouse livers.
    Authors: Yang L, Jung Y, Omenetti A, Witek RP, Choi S, Vandongen HM, Huang J, Alpini GD, Diehl AM
    Stem Cells, 2008;26(8):2104-13.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  12. Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition.
    Authors: Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R
    J. Biol. Chem., 2007;282(32):23337-47.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  13. Stem cells derived from human fetal membranes display multilineage differentiation potential.
    Authors: Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U
    Biol. Reprod., 2007;77(3):577-88.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro.
    Authors: Baharvand H, Hashemi SM, Kazemi Ashtiani S, Farrokhi A
    Int. J. Dev. Biol., 2006;50(7):645-52.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. Isolation and characterization of a stem cell population from adult human liver.
    Authors: Herrera MB, Bruno S, Buttiglieri S, Tetta C, Gatti S, Deregibus MC, Bussolati B, Camussi G
    Stem Cells, 2006;24(12):2840-50.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 15 Citations
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Average Rating: 5 (Based on 1 review)

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Summary

ApplicationELISA
Sample TestedHuman recombinant antibody
SpeciesHuman

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